FastQCFastQC Report
Fri 10 Feb 2017
ERR1632602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543172
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10310.18981096227346034No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8640.15906563666757492No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7900.14544195945299093No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7530.13863012084569898No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7020.12924082979240462No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6730.12390182115425685No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6690.12316540616968474No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6270.11543304883167763No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6210.11432842635481946No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6140.11303970013181827No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5610.10328220158623788No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5610.10328220158623788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.030.5785121
TTTAGAC250.00549382229.63
CCGCTTA358.863349E-426.4285725
GTTATAT358.863349E-426.428571
TAGCGAA451.3219572E-424.66666610
TCGGGTA451.3219572E-424.66666625
GGACCGT753.7332575E-722.26
GTGATTC705.0937524E-621.14285716
ATCTTAC705.0937524E-621.1428571
CGAACTA806.946211E-720.812524
TTCTAAG450.003823249420.5555552
CCGGAAT450.003823249420.5555553
TATGTTT450.003823249420.5555555
CTTATAC5500.020.51818337
AGTATTA555.138295E-420.18181815
TATGAGC555.138295E-420.1818184
GTAGCGA555.138295E-420.1818189
CAGTATT555.138295E-420.18181814
ACCTCTA656.89357E-519.92307531
GTATCAA9300.019.8924732
Produced by FastQC (version 0.11.5)