##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632602.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543172
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09845868343729	34.0	33.0	34.0	31.0	34.0
2	33.23420389858093	34.0	33.0	34.0	31.0	34.0
3	33.30796506447313	34.0	34.0	34.0	31.0	34.0
4	36.56945497926992	37.0	37.0	37.0	35.0	37.0
5	36.547307298608914	37.0	37.0	37.0	35.0	37.0
6	36.58871407215394	37.0	37.0	37.0	35.0	37.0
7	36.5810884949887	37.0	37.0	37.0	35.0	37.0
8	36.55575213744449	37.0	37.0	37.0	35.0	37.0
9	38.3515368980728	39.0	39.0	39.0	37.0	39.0
10	38.38760098090476	39.0	39.0	39.0	37.0	39.0
11	38.45623485746688	39.0	39.0	39.0	37.0	39.0
12	38.420562915614205	39.0	39.0	39.0	37.0	39.0
13	38.45313455038183	39.0	39.0	39.0	37.0	39.0
14	40.03696250911314	41.0	40.0	41.0	38.0	41.0
15	40.02851951131502	41.0	40.0	41.0	38.0	41.0
16	40.00144337336976	41.0	40.0	41.0	38.0	41.0
17	40.00082478478272	41.0	40.0	41.0	38.0	41.0
18	39.99046342594979	41.0	40.0	41.0	38.0	41.0
19	39.992880708136646	41.0	40.0	41.0	38.0	41.0
20	39.95973651071852	41.0	40.0	41.0	38.0	41.0
21	39.93386809334796	41.0	40.0	41.0	38.0	41.0
22	39.90178433350762	41.0	40.0	41.0	38.0	41.0
23	39.87181040259807	41.0	40.0	41.0	38.0	41.0
24	39.85108584389475	41.0	40.0	41.0	38.0	41.0
25	39.80789142297468	41.0	40.0	41.0	38.0	41.0
26	39.727121427466805	41.0	40.0	41.0	38.0	41.0
27	39.66066733925902	41.0	40.0	41.0	37.0	41.0
28	39.61736429712872	41.0	40.0	41.0	37.0	41.0
29	39.57786483839373	41.0	40.0	41.0	37.0	41.0
30	39.52656985264336	41.0	40.0	41.0	37.0	41.0
31	39.46228266552768	41.0	40.0	41.0	37.0	41.0
32	39.414351255219344	41.0	39.0	41.0	36.0	41.0
33	39.34757314441834	41.0	39.0	41.0	36.0	41.0
34	39.31112612579441	41.0	39.0	41.0	36.0	41.0
35	39.237121942957295	41.0	39.0	41.0	35.0	41.0
36	39.187472844697446	41.0	39.0	41.0	35.0	41.0
37	39.127024220688845	41.0	39.0	41.0	35.0	41.0
38	39.04712319486277	41.0	39.0	41.0	35.0	41.0
39	38.95722717665859	41.0	39.0	41.0	35.0	41.0
40	38.86958090623228	40.0	39.0	41.0	35.0	41.0
41	38.81777227103017	40.0	38.0	41.0	35.0	41.0
42	38.74469413003616	40.0	38.0	41.0	35.0	41.0
43	38.01125794407665	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	7.0
19	9.0
20	28.0
21	52.0
22	96.0
23	169.0
24	312.0
25	440.0
26	604.0
27	872.0
28	1119.0
29	1629.0
30	2133.0
31	2877.0
32	3725.0
33	5076.0
34	7750.0
35	12622.0
36	19907.0
37	38121.0
38	99979.0
39	345642.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.220747755775335	16.349885487469898	12.148822104232176	28.280544652522586
2	21.389173226896823	18.877998129505936	32.036076970094186	27.69675167350305
3	21.5071837281745	19.466761909671337	28.05777911968953	30.96827524246463
4	16.11220755119925	15.09687539122046	32.925298063965	35.865618993615286
5	17.55926299588344	32.51456260631991	32.75224054259056	17.173933855206087
6	35.89470738550588	32.65908404704219	14.698106677074666	16.748101890377264
7	31.387295368686164	26.776601150280204	19.50818525255352	22.32791822848011
8	27.970513944017732	30.609457041231874	19.391095270006552	22.02893374474384
9	28.24648545948613	12.8729021378127	17.403695330392583	41.47691707230859
10	19.778817759383767	23.041136141038198	29.726679578476062	27.453366521101973
11	39.72664275772683	19.178271339465216	18.62743293100528	22.46765297180267
12	23.43972075143785	23.06359679806765	26.199435906121813	27.297246544372683
13	34.65918714514003	17.462792632904495	20.54248009838504	27.335540123570436
14	24.653148542266536	19.05749928199539	22.51809739824586	33.77125477749221
15	29.487344708490127	23.77350084319516	19.86645114254785	26.872703305766866
16	26.733336769936596	23.137054192778713	21.913132488419873	28.216476548864815
17	27.425382751688232	23.12453513804099	21.712827612616262	27.73725449765452
18	26.82649326548497	21.484354863652765	23.064149109306076	28.62500276155619
19	28.541235557061118	22.011627992606396	22.662434735221993	26.784701715110497
20	29.212477815498588	21.105469354090417	22.69336416457402	26.988688665836975
21	27.915466923920967	22.02709270728241	21.833599670086087	28.22384069871054
22	28.510306127709086	22.49434801499341	21.218693157968378	27.776652699329123
23	28.377199119247678	22.06115190031887	22.095763404593757	27.4658855758397
24	28.374805770547816	21.634583520505476	21.78738962980419	28.20322107914252
25	28.461886842473472	21.770820292651315	22.46415500062595	27.303137864249262
26	28.359341055871806	22.40321666065261	21.965049744832207	27.272392538643373
27	27.685337241242188	21.873918390491408	23.07703637153609	27.36370799673032
28	26.932352919517204	22.523252303137863	22.034272753381988	28.510122023962943
29	27.942898382096278	22.92699181842952	22.078273548710172	27.051836250764033
30	27.13873321894354	22.033720442143554	23.16411744346174	27.663428895451165
31	28.06274992083539	21.89766777374386	22.332152614641405	27.70742969077935
32	26.228156090520127	22.026540396043977	22.688945674666588	29.056357838769305
33	26.595995375313898	21.63900201041291	23.835543805645358	27.92945880862784
34	26.956102302769658	21.44016996457844	23.714219436937103	27.889508295714798
35	26.55420382493943	22.76203486188537	23.793384047778606	26.890377265396598
36	26.883933634281593	21.509208869382075	24.018542929311526	27.588314567024806
37	26.871782787036153	21.0373509680175	24.24683157452888	27.84403467041747
38	26.09799474199701	20.651837723594	25.325863630673158	27.92430390373583
39	25.442585405727836	20.064362669651604	26.381146303564982	28.111905621055577
40	25.330098016834445	20.815690057661293	27.218266037277328	26.635945888226935
41	24.07358994940829	20.02717371293071	27.842745944194476	28.056490393466525
42	22.547738101374886	21.0728829910231	28.629237147717486	27.750141759884528
43	22.418865479074768	20.96094791336814	28.373332940578678	28.246853666978417
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	10.5
4	17.0
5	17.0
6	25.0
7	33.0
8	30.5
9	28.0
10	45.0
11	62.0
12	62.0
13	147.5
14	233.0
15	349.5
16	466.0
17	440.5
18	415.0
19	415.0
20	523.5
21	632.0
22	541.0
23	450.0
24	563.5
25	677.0
26	677.0
27	860.5
28	1044.0
29	1546.5
30	2049.0
31	2725.0
32	3401.0
33	3401.0
34	4706.0
35	6011.0
36	7200.5
37	8390.0
38	10974.5
39	13559.0
40	13559.0
41	15624.5
42	17690.0
43	21147.0
44	24604.0
45	30646.5
46	36689.0
47	36689.0
48	40996.5
49	45304.0
50	49871.0
51	54438.0
52	56587.5
53	58737.0
54	58737.0
55	56180.0
56	53623.0
57	50955.0
58	48287.0
59	45994.5
60	43702.0
61	43702.0
62	40994.0
63	38286.0
64	32138.0
65	25990.0
66	22506.0
67	19022.0
68	19022.0
69	16199.0
70	13376.0
71	11351.0
72	9326.0
73	7798.0
74	6270.0
75	6270.0
76	5244.0
77	4218.0
78	3521.0
79	2824.0
80	2307.5
81	1791.0
82	1791.0
83	1280.5
84	770.0
85	580.0
86	390.0
87	294.5
88	199.0
89	199.0
90	153.5
91	108.0
92	74.0
93	40.0
94	25.5
95	11.0
96	11.0
97	6.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	543172.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.807464391117264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55749033593258	44.32516633972187
2	6.940037214025639	7.190914616773241
3	2.2200461829838263	3.4504489071471114
4	1.197237064133933	2.48103266271378
5	0.7052491615910201	1.8268585412996041
6	0.4988950241460592	1.550789171901153
7	0.37948726841754254	1.3762191201796936
8	0.2978639935729571	1.2345262592341568
9	0.22013507293784487	1.0264175957217059
>10	1.6961587177841526	17.322664675932646
>50	0.17479923219911325	6.3337629191545215
>100	0.10795371792747951	10.219098019133977
>500	0.004289551705727663	1.4711678777840254
>1k	3.574626421439719E-4	0.19093329330253403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1031	0.18981096227346034	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	864	0.15906563666757492	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	790	0.14544195945299093	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	753	0.13863012084569898	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	702	0.12924082979240462	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	673	0.12390182115425685	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	669	0.12316540616968474	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	627	0.11543304883167763	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	621	0.11432842635481946	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	614	0.11303970013181827	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	561	0.10328220158623788	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	561	0.10328220158623788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.68207492286053E-4	0.0	0.0	0.0	0.0
6	3.68207492286053E-4	0.0	0.0	0.0	0.0
7	3.68207492286053E-4	0.0	0.0	0.0	0.0
8	5.523112384290795E-4	0.0	0.0	0.0	0.0
9	5.523112384290795E-4	0.0	0.0	0.0	0.0
10	5.523112384290795E-4	0.0	0.0	0.0	0.0
11	5.523112384290795E-4	0.0	0.0	0.0	0.0
12	5.523112384290795E-4	0.0	0.0	0.0	0.0
13	5.523112384290795E-4	0.0	0.0	1.841037461430265E-4	0.0
14	5.523112384290795E-4	0.0	0.0	1.841037461430265E-4	0.0
15	5.523112384290795E-4	0.0	0.0	3.68207492286053E-4	0.0
16	5.523112384290795E-4	0.0	0.0	5.523112384290795E-4	0.0
17	7.36414984572106E-4	0.0	0.0	7.36414984572106E-4	0.0
18	7.36414984572106E-4	0.0	0.0	7.36414984572106E-4	0.0
19	7.36414984572106E-4	0.0	0.0	0.001104622476858159	0.0
20	7.36414984572106E-4	0.0	0.0	0.001472829969144212	0.0
21	7.36414984572106E-4	0.0	0.0	0.0027615561921453978	0.0
22	7.36414984572106E-4	0.0	0.0	0.00423438616128961	1.841037461430265E-4
23	7.36414984572106E-4	0.0	0.0	0.006075423622719875	1.841037461430265E-4
24	7.36414984572106E-4	0.0	0.0	0.00736414984572106	1.841037461430265E-4
25	7.36414984572106E-4	0.0	0.0	0.008652876068722247	1.841037461430265E-4
26	7.36414984572106E-4	0.0	0.0	0.012519054737725804	1.841037461430265E-4
27	0.001104622476858159	0.0	0.0	0.03461150427488899	1.841037461430265E-4
28	0.001104622476858159	0.0	0.0	0.12298130242354172	1.841037461430265E-4
29	0.001104622476858159	0.0	0.0	0.25019699100837306	1.841037461430265E-4
30	0.001104622476858159	0.0	0.0	0.4129447025988085	1.841037461430265E-4
31	0.0012887262230011857	0.0	0.0	0.9411383502831515	1.841037461430265E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	605	0.0	30.578512	1
TTTAGAC	25	0.005493822	29.6	3
CCGCTTA	35	8.863349E-4	26.42857	25
GTTATAT	35	8.863349E-4	26.42857	1
TAGCGAA	45	1.3219572E-4	24.666666	10
TCGGGTA	45	1.3219572E-4	24.666666	25
GGACCGT	75	3.7332575E-7	22.2	6
GTGATTC	70	5.0937524E-6	21.142857	16
ATCTTAC	70	5.0937524E-6	21.142857	1
CGAACTA	80	6.946211E-7	20.8125	24
TTCTAAG	45	0.0038232494	20.555555	2
CCGGAAT	45	0.0038232494	20.555555	3
TATGTTT	45	0.0038232494	20.555555	5
CTTATAC	550	0.0	20.518183	37
AGTATTA	55	5.138295E-4	20.181818	15
TATGAGC	55	5.138295E-4	20.181818	4
GTAGCGA	55	5.138295E-4	20.181818	9
CAGTATT	55	5.138295E-4	20.181818	14
ACCTCTA	65	6.89357E-5	19.923075	31
GTATCAA	930	0.0	19.892473	2
>>END_MODULE