FastQCFastQC Report
Fri 10 Feb 2017
ERR1632601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44720
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT1000.22361359570661896RNA PCR Primer, Index 41 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA940.2101967799642218No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC850.19007155635062611No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG780.1744186046511628No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG680.1520572450805009No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA680.1520572450805009No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA680.1520572450805009No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT620.13864042933810375No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA620.13864042933810375No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT610.13640429338103754No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA610.13640429338103754No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA610.13640429338103754No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC590.1319320214669052No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG580.12969588550983902No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG570.1274597495527728No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT570.1274597495527728No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA560.12522361359570663No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA560.12522361359570663No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA550.12298747763864043No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC540.12075134168157424No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT530.11851520572450805No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA520.11627906976744186No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT520.11627906976744186No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC520.11627906976744186No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG510.11404293381037567No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG500.11180679785330948No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG500.11180679785330948No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC500.11180679785330948No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT480.1073345259391771No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC470.1050983899821109No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA460.10286225402504473No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA460.10286225402504473No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG450.10062611806797854No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC450.10062611806797854No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA450.10062611806797854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG250.005440713429.59999827
TCCGTGC358.7361934E-426.428578
TGGGACA358.7361934E-426.428574
CTCCGTG358.7361934E-426.428577
GGTATCA753.5773155E-722.1999991
CCAGCAG450.00376943420.55555514
TACGGGA450.00376943420.55555516
TGATTCA450.00376943420.5555554
CTGATTC450.00376943420.5555553
GCCAGCA450.00376943420.55555513
ACGGAGG450.00376943420.55555531
CGACATT450.00376943420.55555530
TCTTATA656.7144625E-519.92307737
GTATCAA951.5881051E-719.4736842
GACATTG500.006932209718.49999831
ACGACAT500.006932209718.49999829
CTCTTAT1209.768519E-816.95833237
CTGGGCT750.004022945614.79999915
ATCAACG1356.2354084E-613.7037044
TATCAAC1409.078603E-613.2142853
Produced by FastQC (version 0.11.5)