##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632601.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44720
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.022070661896244	34.0	33.0	34.0	31.0	34.0
2	33.17063953488372	34.0	33.0	34.0	31.0	34.0
3	33.25360017889088	34.0	34.0	34.0	31.0	34.0
4	36.54394007155635	37.0	37.0	37.0	35.0	37.0
5	36.5037343470483	37.0	37.0	37.0	35.0	37.0
6	36.542307692307695	37.0	37.0	37.0	35.0	37.0
7	36.51873881932021	37.0	37.0	37.0	35.0	37.0
8	36.49376118067978	37.0	37.0	37.0	35.0	37.0
9	38.30304114490161	39.0	39.0	39.0	37.0	39.0
10	38.3363595706619	39.0	39.0	39.0	37.0	39.0
11	38.42081842576029	39.0	39.0	39.0	37.0	39.0
12	38.33450357781753	39.0	39.0	39.0	37.0	39.0
13	38.38423524150269	39.0	39.0	39.0	37.0	39.0
14	39.9466457960644	41.0	40.0	41.0	38.0	41.0
15	39.936292486583184	41.0	40.0	41.0	38.0	41.0
16	39.911314847942755	41.0	40.0	41.0	38.0	41.0
17	39.88611359570662	41.0	40.0	41.0	38.0	41.0
18	39.899888193202145	41.0	40.0	41.0	38.0	41.0
19	39.881820214669055	41.0	40.0	41.0	38.0	41.0
20	39.87475402504472	41.0	40.0	41.0	38.0	41.0
21	39.846176207513416	41.0	40.0	41.0	38.0	41.0
22	39.806574239713775	41.0	40.0	41.0	38.0	41.0
23	39.78045617173524	41.0	40.0	41.0	38.0	41.0
24	39.7604427549195	41.0	40.0	41.0	38.0	41.0
25	39.715943649373884	41.0	40.0	41.0	38.0	41.0
26	39.62468694096601	41.0	40.0	41.0	37.0	41.0
27	39.54284436493739	41.0	40.0	41.0	37.0	41.0
28	39.50711091234347	41.0	40.0	41.0	37.0	41.0
29	39.45881037567084	41.0	39.0	41.0	37.0	41.0
30	39.425559033989266	41.0	39.0	41.0	37.0	41.0
31	39.344879248658316	41.0	39.0	41.0	36.0	41.0
32	39.29673524150268	41.0	39.0	41.0	36.0	41.0
33	39.23273703041145	41.0	39.0	41.0	36.0	41.0
34	39.14809928443649	41.0	39.0	41.0	35.0	41.0
35	39.095080500894454	41.0	39.0	41.0	35.0	41.0
36	39.01080053667263	41.0	39.0	41.0	35.0	41.0
37	38.94780858676207	40.0	39.0	41.0	35.0	41.0
38	38.88047853309481	40.0	38.0	41.0	35.0	41.0
39	38.78450357781753	40.0	38.0	41.0	35.0	41.0
40	38.6882602862254	40.0	38.0	41.0	35.0	41.0
41	38.61520572450805	40.0	38.0	41.0	35.0	41.0
42	38.5270572450805	40.0	38.0	41.0	35.0	41.0
43	37.81978980322003	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	4.0
22	7.0
23	16.0
24	26.0
25	41.0
26	52.0
27	64.0
28	99.0
29	130.0
30	187.0
31	280.0
32	351.0
33	528.0
34	694.0
35	1174.0
36	1832.0
37	3803.0
38	8637.0
39	26791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22406082289803	16.26118067978533	12.063953488372094	27.450805008944545
2	21.58542039355993	18.770125223613597	31.967799642218246	27.67665474060823
3	22.063953488372093	18.55098389982111	28.014311270125226	31.370751341681576
4	15.688729874776385	15.431574239713775	32.18023255813954	36.6994633273703
5	18.21556350626118	31.793381037567087	32.48211091234347	17.508944543828264
6	37.41726296958855	31.757602862254025	14.767441860465116	16.05769230769231
7	32.748211091234346	26.156082289803216	18.46824686940966	22.627459749552774
8	28.622540250447226	29.890429338103758	19.71824686940966	21.768783542039355
9	28.255813953488374	11.773255813953488	17.00357781753131	42.96735241502683
10	19.19499105545617	23.18872987477639	29.342576028622542	28.273703041144906
11	40.818425760286225	18.249105545617173	17.757155635062613	23.17531305903399
12	23.1350626118068	22.822003577817533	25.919051878354203	28.123881932021465
13	35.974955277280856	16.634615384615383	20.078264758497316	27.312164579606442
14	25.507602862254025	19.132379248658317	22.061717352415027	33.298300536672635
15	29.38953488372093	23.407871198568873	19.756261180679786	27.44633273703041
16	28.282647584973162	22.155635062611807	21.65026833631485	27.911449016100182
17	27.59838998211091	22.95840787119857	20.84525939177102	28.597942754919497
18	26.905187835420392	20.43381037567084	22.777280858676207	29.88372093023256
19	30.11180679785331	21.82468694096601	21.623434704830053	26.440071556350624
20	30.970483005366727	20.219141323792485	21.762075134168157	27.04830053667263
21	27.886851520572453	21.478085867620752	21.18067978533095	29.45438282647585
22	29.490161001788913	21.784436493738816	20.308586762075134	28.41681574239714
23	27.678890876565294	21.744186046511626	21.784436493738816	28.792486583184257
24	28.606887298747765	21.216457960644007	21.556350626118068	28.620304114490164
25	28.752236135957066	21.61449016100179	22.05948121645796	27.57379248658318
26	28.877459749552774	22.003577817531305	20.601520572450806	28.517441860465116
27	28.430232558139533	21.703935599284435	21.940966010733455	27.924865831842578
28	27.110912343470485	22.790697674418606	21.11583184257603	28.982558139534888
29	28.405635062611807	23.275939177101968	21.399821109123433	26.91860465116279
30	28.222271914132378	20.80948121645796	22.25402504472272	28.71422182468694
31	27.589445438282645	20.894454382826474	22.128801431127012	29.38729874776386
32	26.348389982110916	21.815742397137747	22.495527728085868	29.340339892665472
33	26.569767441860463	20.657423971377458	23.389982110912342	29.382826475849733
34	27.497763864042934	21.462432915921287	23.144007155635062	27.895796064400713
35	27.403846153846157	21.876118067978535	22.947227191413237	27.772808586762075
36	27.131037567084075	20.98613595706619	22.898032200357783	28.98479427549195
37	26.12701252236136	20.85644007155635	24.95304114490161	28.06350626118068
38	25.813953488372093	20.239266547406082	24.572898032200357	29.37388193202147
39	25.34660107334526	20.45393559928444	26.308139534883722	27.891323792486585
40	26.180679785330945	19.81887298747764	26.873881932021465	27.126565295169947
41	24.033989266547408	19.760733452593918	27.817531305903398	28.387745974955276
42	22.13550983899821	21.082289803220036	28.866279069767444	27.91592128801431
43	22.44856887298748	20.149821109123433	27.76610017889088	29.63550983899821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.0
7	8.0
8	5.0
9	2.0
10	2.5
11	3.0
12	3.0
13	14.0
14	25.0
15	29.5
16	34.0
17	41.0
18	48.0
19	48.0
20	51.0
21	54.0
22	48.5
23	43.0
24	51.0
25	59.0
26	59.0
27	60.0
28	61.0
29	83.5
30	106.0
31	152.0
32	198.0
33	198.0
34	318.5
35	439.0
36	511.5
37	584.0
38	692.0
39	800.0
40	800.0
41	969.0
42	1138.0
43	1425.0
44	1712.0
45	2219.5
46	2727.0
47	2727.0
48	2980.0
49	3233.0
50	3767.5
51	4302.0
52	4692.0
53	5082.0
54	5082.0
55	4908.5
56	4735.0
57	4382.5
58	4030.0
59	4055.5
60	4081.0
61	4081.0
62	3794.0
63	3507.0
64	2782.5
65	2058.0
66	1846.5
67	1635.0
68	1635.0
69	1526.5
70	1418.0
71	1363.0
72	1308.0
73	1054.5
74	801.0
75	801.0
76	504.0
77	207.0
78	163.5
79	120.0
80	95.0
81	70.0
82	70.0
83	55.5
84	41.0
85	33.5
86	26.0
87	19.5
88	13.0
89	13.0
90	9.5
91	6.0
92	4.5
93	3.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	44720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.158318425760285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55027422303473	52.32110912343471
2	6.654478976234003	8.13953488372093
3	2.340036563071298	4.293381037567084
4	1.4223034734917732	3.479427549194991
5	0.8007312614259597	2.4485688729874777
6	0.5447897623400365	1.9991055456171736
7	0.3875685557586837	1.6592128801431127
8	0.3180987202925046	1.556350626118068
9	0.226691042047532	1.2477638640429338
>10	1.6636197440585008	19.322450805008945
>50	0.09140767824497258	3.5330948121645798
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	100	0.22361359570661896	RNA PCR Primer, Index 41 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	94	0.2101967799642218	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	85	0.19007155635062611	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	78	0.1744186046511628	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	68	0.1520572450805009	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	68	0.1520572450805009	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	68	0.1520572450805009	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	62	0.13864042933810375	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	62	0.13864042933810375	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	61	0.13640429338103754	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	61	0.13640429338103754	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	61	0.13640429338103754	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	59	0.1319320214669052	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	58	0.12969588550983902	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	57	0.1274597495527728	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	57	0.1274597495527728	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	56	0.12522361359570663	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	56	0.12522361359570663	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	55	0.12298747763864043	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	54	0.12075134168157424	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	53	0.11851520572450805	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	52	0.11627906976744186	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	52	0.11627906976744186	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	52	0.11627906976744186	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	51	0.11404293381037567	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	50	0.11180679785330948	No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG	50	0.11180679785330948	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	50	0.11180679785330948	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	48	0.1073345259391771	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	47	0.1050983899821109	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	46	0.10286225402504473	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	46	0.10286225402504473	No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG	45	0.10062611806797854	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	45	0.10062611806797854	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	45	0.10062611806797854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0022361359570661895	0.0
17	0.0	0.0	0.0	0.0022361359570661895	0.0
18	0.0	0.0	0.0	0.004472271914132379	0.0
19	0.0	0.0	0.0	0.004472271914132379	0.0
20	0.0	0.0	0.0	0.006708407871198569	0.0
21	0.0022361359570661895	0.0	0.0	0.006708407871198569	0.0
22	0.0022361359570661895	0.0	0.0	0.008944543828264758	0.0
23	0.0022361359570661895	0.0	0.0	0.011180679785330949	0.0
24	0.0022361359570661895	0.0	0.0	0.020125223613595707	0.0
25	0.0022361359570661895	0.0	0.0	0.024597495527728087	0.0
26	0.0022361359570661895	0.0	0.0	0.029069767441860465	0.0
27	0.0022361359570661895	0.0	0.0	0.05366726296958855	0.0
28	0.0022361359570661895	0.0	0.0	0.17889087656529518	0.0
29	0.0022361359570661895	0.0	0.0	0.35107334525939177	0.0
30	0.0022361359570661895	0.0	0.0	0.5299642218246869	0.0
31	0.0022361359570661895	0.0	0.0	1.1068872987477638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	0.0054407134	29.599998	27
TCCGTGC	35	8.7361934E-4	26.42857	8
TGGGACA	35	8.7361934E-4	26.42857	4
CTCCGTG	35	8.7361934E-4	26.42857	7
GGTATCA	75	3.5773155E-7	22.199999	1
CCAGCAG	45	0.003769434	20.555555	14
TACGGGA	45	0.003769434	20.555555	16
TGATTCA	45	0.003769434	20.555555	4
CTGATTC	45	0.003769434	20.555555	3
GCCAGCA	45	0.003769434	20.555555	13
ACGGAGG	45	0.003769434	20.555555	31
CGACATT	45	0.003769434	20.555555	30
TCTTATA	65	6.7144625E-5	19.923077	37
GTATCAA	95	1.5881051E-7	19.473684	2
GACATTG	50	0.0069322097	18.499998	31
ACGACAT	50	0.0069322097	18.499998	29
CTCTTAT	120	9.768519E-8	16.958332	37
CTGGGCT	75	0.0040229456	14.799999	15
ATCAACG	135	6.2354084E-6	13.703704	4
TATCAAC	140	9.078603E-6	13.214285	3
>>END_MODULE