FastQCFastQC Report
Fri 10 Feb 2017
ERR1632600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79274
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5640.7114564674420365No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3740.47178141635340715No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3000.3784342911925726No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2920.36834271009410396No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2380.3002245376794409No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2380.3002245376794409No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2370.29896309004213234No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2290.28887150894366376No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2190.276257032570578No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2160.2724726896586523No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2140.2699497943840351No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2020.25481242273633226No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1990.25102807982440645No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1980.2497666321870979No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1970.24850518454978934No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1930.24345939400055505No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1870.23589070817670357No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1790.22579912707823496No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1760.22201478416630926No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT1720.21696899361707497RNA PCR Primer, Index 33 (95% over 22bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1660.2094003077932235No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1650.20813886015591493No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1570.19804727905744632No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1520.19174004087090346No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1500.1892171455962863No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1480.18669425032166914No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1470.18543280268436058No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1460.18417135504705198No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1440.18164845977243485No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1420.1791255644978177No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1420.1791255644978177No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1400.17660266922320053No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1380.1740797739485834No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1370.17281832631127483No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1360.17155687867396624No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1360.17155687867396624No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1340.16903398339934908No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1340.16903398339934908No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1300.1639881928501148No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1250.1576809546635719No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1220.1538966117516462No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1210.1526351641143376No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1190.15011226883972045No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1180.1488508212024119No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1140.1438050306531776No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1110.14002068774125187No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1110.14002068774125187No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1100.13875924010394328No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1070.13497489719201755No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1060.13371344955470899No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1060.13371344955470899No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1060.13371344955470899No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1040.13119055428009183No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1030.12992910664278326No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1010.12740621136816613No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC1010.12740621136816613No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC980.12362186845624037No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG980.12362186845624037No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA970.12236042081893181No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA970.12236042081893181No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT970.12236042081893181No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA950.11983752554431465No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT950.11983752554431465No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG930.1173146302696975No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC900.11353028735777176No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA900.11353028735777176No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA900.11353028735777176No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA890.1122688397204632No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC890.1122688397204632No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC890.1122688397204632No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG880.11100739208315463No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA880.11100739208315463No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG860.10848449680853749No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT860.10848449680853749No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT850.1072230491712289No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA850.1072230491712289No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC850.1072230491712289No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT840.10596160153392031No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA840.10596160153392031No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT830.10470015389661175No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG830.10470015389661175No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG810.1021772586219946No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC810.1021772586219946No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG810.1021772586219946No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC810.1021772586219946No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA800.10091581098468604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCA200.00183161637.05
GCTAAAC200.00183161637.03
GGGCTAA200.00183161637.01
GGTATCA1500.029.61
TGCACCG250.00546590129.59999812
AAACCAT250.00546590129.5999986
AACTGTC250.00546590129.59999837
CACCGAA250.00546590129.59999814
AACCATG250.00546590129.5999987
CTAAACC250.00546590129.5999984
GCACCGA250.00546590129.59999813
AGCGTGT250.00546590129.59999822
TTCTTCT754.456524E-1027.13333315
TCTTCTG754.456524E-1027.13333316
GGTAATA2150.024.95349125
GCGGTAA2200.024.38636423
CGCGGTA2200.024.38636422
AACGTCA851.906301E-923.94117528
GTAACGT851.906301E-923.94117526
CCAGCAG2400.023.89583214
Produced by FastQC (version 0.11.5)