FastQCFastQC Report
Fri 10 Feb 2017
ERR1632599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100011
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC2200.21997580266170721No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2090.20897701252862183No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG1710.1709811920688724No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG1640.16398196198418175No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1540.15398306186319505No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA1510.15098339182689904No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG1480.14798372179060304No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC1470.14698383177850438No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT1460.1459839417664057No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA1340.13398526162122165No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA1330.132985371609123No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC1280.12798592154862964No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1240.12398636150023497No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT1120.11198768135505094No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG1110.11098779134295228No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC1100.10998790133085361No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT1090.10898801131875493No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1080.10798812130665626No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA1080.10798812130665626No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1070.1069882312945576No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1070.1069882312945576No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG1060.10598834128245893No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC1040.10398856125826159No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA1040.10398856125826159No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG1020.10198878123406425No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC1010.10098889122196557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA601.2405508E-930.8333321
TATACAC451.3114478E-424.66666837
ATCTCCT400.001918950123.1250027
GGTAATA753.6706115E-722.225
CGCGGTA753.6706115E-722.222
GCGGTAA753.6706115E-722.223
CTCACAG502.678274E-422.19999932
GCTCACA705.0255057E-621.14285931
GCCGCGA759.127858E-619.73333416
CGGTAAT851.2222154E-619.58823624
GTATCAA951.6382546E-719.4736842
CTTATAC1355.2750693E-1119.18518637
GAGCTCA701.2051337E-418.529
TACCACG701.2051337E-418.54
CGTGCCA902.1125306E-618.510
CCCTACT701.2051337E-418.518
GGATACC701.2051337E-418.51
CGCGAAA701.2051337E-418.518
ATATCAG701.2051337E-418.55
AGCATCT701.2051337E-418.55
Produced by FastQC (version 0.11.5)