##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100011
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03361630220676	34.0	33.0	34.0	31.0	34.0
2	33.16945136035036	34.0	33.0	34.0	31.0	34.0
3	33.254901960784316	34.0	34.0	34.0	31.0	34.0
4	36.54275029746728	37.0	37.0	37.0	35.0	37.0
5	36.50353461119277	37.0	37.0	37.0	35.0	37.0
6	36.541920388757234	37.0	37.0	37.0	35.0	37.0
7	36.53155152933177	37.0	37.0	37.0	35.0	37.0
8	36.50460449350572	37.0	37.0	37.0	35.0	37.0
9	38.316225215226325	39.0	39.0	39.0	37.0	39.0
10	38.3370829208787	39.0	39.0	39.0	37.0	39.0
11	38.41263461019288	39.0	39.0	39.0	37.0	39.0
12	38.33001369849317	39.0	39.0	39.0	37.0	39.0
13	38.38393776684565	39.0	39.0	39.0	37.0	39.0
14	39.94383617802042	41.0	40.0	41.0	38.0	41.0
15	39.93666696663367	41.0	40.0	41.0	38.0	41.0
16	39.9184789673136	41.0	40.0	41.0	38.0	41.0
17	39.886622471528135	41.0	40.0	41.0	38.0	41.0
18	39.885642579316276	41.0	40.0	41.0	38.0	41.0
19	39.85379608243093	41.0	40.0	41.0	38.0	41.0
20	39.855455899851016	41.0	40.0	41.0	38.0	41.0
21	39.827478977312495	41.0	40.0	41.0	38.0	41.0
22	39.80336163022068	41.0	40.0	41.0	38.0	41.0
23	39.77228504864465	41.0	40.0	41.0	38.0	41.0
24	39.74576796552379	41.0	40.0	41.0	38.0	41.0
25	39.712121666616675	41.0	40.0	41.0	38.0	41.0
26	39.62931077581466	41.0	40.0	41.0	37.0	41.0
27	39.522472528021915	41.0	40.0	41.0	37.0	41.0
28	39.49147593764686	41.0	39.0	41.0	37.0	41.0
29	39.425443201247866	41.0	39.0	41.0	37.0	41.0
30	39.387957324694284	41.0	39.0	41.0	37.0	41.0
31	39.289258181600026	41.0	39.0	41.0	36.0	41.0
32	39.2361240263571	41.0	39.0	41.0	36.0	41.0
33	39.17201107878133	41.0	39.0	41.0	35.0	41.0
34	39.09041005489396	41.0	39.0	41.0	35.0	41.0
35	39.03558608553059	41.0	39.0	41.0	35.0	41.0
36	38.958274589795124	40.0	39.0	41.0	35.0	41.0
37	38.89428162902081	40.0	39.0	41.0	35.0	41.0
38	38.812970573236946	40.0	38.0	41.0	35.0	41.0
39	38.68984411714711	40.0	38.0	41.0	35.0	41.0
40	38.60642329343772	40.0	38.0	41.0	35.0	41.0
41	38.540760516343205	40.0	38.0	41.0	35.0	41.0
42	38.473277939426666	40.0	38.0	41.0	35.0	41.0
43	37.70735219125896	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	7.0
22	25.0
23	35.0
24	65.0
25	100.0
26	119.0
27	168.0
28	245.0
29	328.0
30	432.0
31	603.0
32	830.0
33	1137.0
34	1647.0
35	2541.0
36	4103.0
37	8618.0
38	20164.0
39	58835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10103888572257	15.690274069852316	11.518732939376669	27.689954105048443
2	21.669616342202357	18.65594784573697	31.867494575596684	27.806941236463988
3	22.410534841167472	18.09300976892542	27.135015148333686	32.361440241573426
4	15.668276489586145	14.774374818769937	32.19145893951666	37.365889752127266
5	18.69094399616042	32.12146663866975	31.347551769305376	17.840037595864455
6	37.85783563807981	31.179570247272796	14.660387357390686	16.3022067572567
7	33.011368749437565	26.077131515533292	17.95702472728	22.95447500774915
8	28.586855445900948	29.536750957394688	19.644839067702552	22.23155452900181
9	28.25589185189629	11.87869334373219	16.579176290608032	43.286238513763486
10	19.36087030426653	22.718500964893863	29.487756346801852	28.432872384037754
11	40.76051634320225	18.52796192418834	17.571067182609912	23.1404545499995
12	23.38042815290318	22.696503384627693	25.38620751717311	28.536860945296016
13	36.21801601823799	16.631170571237163	19.941806401295857	27.209007009228987
14	25.821159672436032	18.192998770135286	21.747607763146053	34.23823379428263
15	29.072801991780906	23.375428702842687	19.739828618851927	27.811940686524483
16	28.72184059753427	21.866594674585794	21.309655937846834	28.101908790033097
17	27.948925618182002	22.227554968953413	21.04368519462859	28.779834218235994
18	27.567967523572406	20.021797602263753	22.626511083780784	29.78372379038306
19	30.465648778634353	21.933587305396408	21.410644829068804	26.19011908690044
20	30.805611382747898	20.294767575566688	21.38764735879053	27.51197368289488
21	28.139904610492845	21.379648238693743	20.814710381857996	29.665736768955416
22	29.662737098919116	21.281659017508073	20.057793642699302	28.997810240873505
23	27.98492165861755	21.145673975862657	21.55562888082311	29.313775484696684
24	28.515863255041946	20.937696853346132	21.311655717871034	29.23478417374089
25	28.873823879373266	21.153673095959444	21.721610622831488	28.2508924018358
26	29.508754037055922	21.655617882032978	20.324764275929645	28.51086380498145
27	28.882822889482156	21.061683214846365	21.48063712991571	28.57485676575577
28	27.652958174600794	22.242553319134895	21.040685524592295	29.063802981672016
29	28.439871614122445	22.763496015438303	21.551629320774715	27.245003049664536
30	28.811830698623154	20.651728309885915	21.830598634150245	28.705842357340693
31	27.6499585045645	20.92969773324934	21.80360160382358	29.61674215836258
32	26.187119416864146	21.596624371319155	22.288548259691435	29.927707952125264
33	27.695953445121035	20.45774964753877	22.72150063493016	29.124796272410038
34	27.884932657407685	21.040685524592295	23.126456089830118	27.947925728169903
35	27.974922758496568	21.09567947525772	22.350541440441553	28.578856325804164
36	26.82904880463149	20.44875063742988	22.955474897761246	29.76672566017738
37	26.33510313865475	20.154782973872873	24.97525272220056	28.53486116527182
38	26.04513503514613	19.3848676645569	24.761276259611442	29.808721040685526
39	25.023247442781294	19.585845556988733	27.088020317765043	28.30288668246493
40	26.138124806271314	19.036905940346564	27.243003269640337	27.581965983741792
41	23.541410444851067	19.299877013528512	28.22989471158173	28.928817830038696
42	22.112567617562068	20.505744368119508	29.20578736339003	28.175900650928398
43	22.109567947525772	19.82081970983192	28.124906260311366	29.944706082330946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.5
4	4.0
5	4.0
6	6.0
7	8.0
8	8.0
9	8.0
10	8.5
11	9.0
12	9.0
13	12.0
14	15.0
15	42.0
16	69.0
17	63.0
18	57.0
19	57.0
20	76.5
21	96.0
22	98.0
23	100.0
24	129.0
25	158.0
26	158.0
27	163.5
28	169.0
29	237.0
30	305.0
31	416.0
32	527.0
33	527.0
34	791.0
35	1055.0
36	1177.5
37	1300.0
38	1546.5
39	1793.0
40	1793.0
41	2112.5
42	2432.0
43	3024.0
44	3616.0
45	4515.5
46	5415.0
47	5415.0
48	6055.0
49	6695.0
50	7708.5
51	8722.0
52	9727.0
53	10732.0
54	10732.0
55	10594.0
56	10456.0
57	9736.0
58	9016.0
59	9224.0
60	9432.0
61	9432.0
62	8831.5
63	8231.0
64	6695.5
65	5160.0
66	4723.0
67	4286.0
68	4286.0
69	3878.0
70	3470.0
71	3394.0
72	3318.0
73	2689.0
74	2060.0
75	2060.0
76	1323.0
77	586.0
78	455.0
79	324.0
80	254.5
81	185.0
82	185.0
83	141.5
84	98.0
85	78.5
86	59.0
87	42.5
88	26.0
89	26.0
90	18.5
91	11.0
92	7.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	100011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.933517313095564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.75025449609772	51.71431142574316
2	5.653206650831353	6.663267040625531
3	1.8544282321004413	3.2786393496715363
4	1.0943332202239566	2.5797162312145665
5	0.665083135391924	1.9597844237133917
6	0.4360366474380726	1.5418303986561477
7	0.35629453681710216	1.4698383177850436
8	0.27485578554462164	1.2958574556798752
9	0.20020359687818118	1.0618831928487866
>10	1.501526976586359	17.916029236783952
>50	0.16966406515100102	7.101218865924748
>100	0.04411265693926027	3.4176240613532514
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	220	0.21997580266170721	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	209	0.20897701252862183	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	171	0.1709811920688724	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	164	0.16398196198418175	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	154	0.15398306186319505	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	151	0.15098339182689904	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	148	0.14798372179060304	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	147	0.14698383177850438	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	146	0.1459839417664057	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	134	0.13398526162122165	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	133	0.132985371609123	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	128	0.12798592154862964	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	124	0.12398636150023497	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	112	0.11198768135505094	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	111	0.11098779134295228	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	110	0.10998790133085361	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	109	0.10898801131875493	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	108	0.10798812130665626	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	108	0.10798812130665626	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	107	0.1069882312945576	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	107	0.1069882312945576	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	106	0.10598834128245893	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	104	0.10398856125826159	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	104	0.10398856125826159	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	102	0.10198878123406425	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	101	0.10098889122196557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	9.99890012098669E-4	0.0
19	0.0	0.0	0.0	9.99890012098669E-4	0.0
20	0.0	0.0	0.0	9.99890012098669E-4	0.0
21	0.0	0.0	0.0	0.0029996700362960073	0.0
22	0.0	0.0	0.0	0.0029996700362960073	0.0
23	0.0	0.0	0.0	0.0029996700362960073	0.0
24	0.0	0.0	0.0	0.003999560048394676	0.0
25	0.0	0.0	0.0	0.005999340072592015	0.0
26	0.0	0.0	0.0	0.01099879013308536	0.0
27	0.0	0.0	0.0	0.0389957104718481	0.0
28	0.0	0.0	0.0	0.1559828418873924	0.0
29	0.0	0.0	0.0	0.3419623841377448	0.0
30	0.0	0.0	0.0	0.5449400565937746	0.0
31	0.0	0.0	0.0	1.2478627350991391	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	60	1.2405508E-9	30.833332	1
TATACAC	45	1.3114478E-4	24.666668	37
ATCTCCT	40	0.0019189501	23.125002	7
GGTAATA	75	3.6706115E-7	22.2	25
CGCGGTA	75	3.6706115E-7	22.2	22
GCGGTAA	75	3.6706115E-7	22.2	23
CTCACAG	50	2.678274E-4	22.199999	32
GCTCACA	70	5.0255057E-6	21.142859	31
GCCGCGA	75	9.127858E-6	19.733334	16
CGGTAAT	85	1.2222154E-6	19.588236	24
GTATCAA	95	1.6382546E-7	19.473684	2
CTTATAC	135	5.2750693E-11	19.185186	37
GAGCTCA	70	1.2051337E-4	18.5	29
TACCACG	70	1.2051337E-4	18.5	4
CGTGCCA	90	2.1125306E-6	18.5	10
CCCTACT	70	1.2051337E-4	18.5	18
GGATACC	70	1.2051337E-4	18.5	1
CGCGAAA	70	1.2051337E-4	18.5	18
ATATCAG	70	1.2051337E-4	18.5	5
AGCATCT	70	1.2051337E-4	18.5	5
>>END_MODULE