FastQCFastQC Report
Fri 10 Feb 2017
ERR1632598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508146
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6690.13165507550979444No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5290.10410393863181054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.030.833331
GTCTTAC250.00549349429.5999981
ATATACA502.6992284E-422.1999994
TCATGAC502.6992284E-422.1999993
ATTGACT502.6992284E-422.19999931
GGATATA603.7210208E-521.5833342
GATATAC603.7210208E-521.5833343
TATACAA603.7210208E-521.5833345
TTCTAGT450.00382291520.5555552
TAGATTG450.00382291520.5555555
TTTTACG450.00382291520.5555554
TCTATCC450.00382291520.5555553
ATACGGC555.1376666E-420.18181829
CTTCTAG656.892447E-519.9230771
GGGATAT656.892447E-519.9230771
CGATAGT1403.6379788E-1219.82142828
AGTCGGT759.2490955E-619.73333411
GTATCAA6100.019.7131162
GCGGGTA1800.019.52777722
GTTTCGA951.671524E-719.47368415
Produced by FastQC (version 0.11.5)