##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	508146
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05759958752012	34.0	33.0	34.0	31.0	34.0
2	33.19868895947228	34.0	33.0	34.0	31.0	34.0
3	33.278266482467636	34.0	34.0	34.0	31.0	34.0
4	36.55920148933574	37.0	37.0	37.0	35.0	37.0
5	36.52299929547807	37.0	37.0	37.0	35.0	37.0
6	36.56441849389742	37.0	37.0	37.0	35.0	37.0
7	36.55872131237873	37.0	37.0	37.0	35.0	37.0
8	36.53059750544135	37.0	37.0	37.0	35.0	37.0
9	38.321752016152836	39.0	39.0	39.0	37.0	39.0
10	38.354252517977116	39.0	39.0	39.0	37.0	39.0
11	38.42496447871281	39.0	39.0	39.0	37.0	39.0
12	38.39507739901524	39.0	39.0	39.0	37.0	39.0
13	38.42033391977896	39.0	39.0	39.0	37.0	39.0
14	39.99682374750564	41.0	40.0	41.0	38.0	41.0
15	39.9859843430825	41.0	40.0	41.0	38.0	41.0
16	39.96350655126676	41.0	40.0	41.0	38.0	41.0
17	39.96010398586233	41.0	40.0	41.0	38.0	41.0
18	39.94882769912584	41.0	40.0	41.0	38.0	41.0
19	39.95335986114227	41.0	40.0	41.0	38.0	41.0
20	39.921977148299895	41.0	40.0	41.0	38.0	41.0
21	39.89673637104297	41.0	40.0	41.0	38.0	41.0
22	39.87082846268592	41.0	40.0	41.0	38.0	41.0
23	39.83058215552223	41.0	40.0	41.0	38.0	41.0
24	39.81135342991975	41.0	40.0	41.0	38.0	41.0
25	39.77429321494216	41.0	40.0	41.0	38.0	41.0
26	39.69158863791115	41.0	40.0	41.0	38.0	41.0
27	39.63088757955391	41.0	40.0	41.0	37.0	41.0
28	39.58055558835453	41.0	40.0	41.0	37.0	41.0
29	39.5441074022033	41.0	40.0	41.0	37.0	41.0
30	39.500305030444004	41.0	40.0	41.0	37.0	41.0
31	39.44331353587355	41.0	40.0	41.0	37.0	41.0
32	39.39430990305936	41.0	39.0	41.0	36.0	41.0
33	39.33334317302508	41.0	39.0	41.0	36.0	41.0
34	39.29257339426071	41.0	39.0	41.0	36.0	41.0
35	39.228711826915884	41.0	39.0	41.0	35.0	41.0
36	39.1825125062482	41.0	39.0	41.0	35.0	41.0
37	39.133908364918746	41.0	39.0	41.0	35.0	41.0
38	39.052351095944864	41.0	39.0	41.0	35.0	41.0
39	38.97961412664864	41.0	39.0	41.0	35.0	41.0
40	38.915557733407326	40.0	39.0	41.0	35.0	41.0
41	38.868020214662714	40.0	39.0	41.0	35.0	41.0
42	38.80537876909392	40.0	38.0	41.0	35.0	41.0
43	38.08743943669733	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	13.0
20	36.0
21	43.0
22	102.0
23	178.0
24	305.0
25	402.0
26	580.0
27	800.0
28	1095.0
29	1591.0
30	2105.0
31	2839.0
32	3745.0
33	4955.0
34	7333.0
35	11764.0
36	18789.0
37	35865.0
38	94585.0
39	321015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94612178389676	16.413983382728585	12.738858517040377	27.90103631633428
2	20.753287441011047	19.320628323355884	32.70280588649719	27.223278349135878
3	21.183675557812126	19.758494605880987	28.465441034663264	30.592388801643622
4	16.362226604164945	15.298162339170238	31.903822917035658	36.43578813962916
5	17.494184742180398	33.16802651206543	32.34424751941371	16.99354122634046
6	36.16381905987649	32.20117840148304	14.76071837621471	16.87428416242576
7	31.28490630645523	27.4545111050761	19.35093457392167	21.909648014547003
8	27.608600677757966	31.632444218787516	19.42512584965738	21.333829253797138
9	27.746749949817573	13.564605448040524	17.439476056094115	41.249168546047784
10	19.22990636549338	24.5409783802293	30.044908353111115	26.184206901166203
11	38.83096590350018	19.762036894908157	18.933928437889897	22.47306876370177
12	23.227182738819156	23.466877629657617	26.379426385330202	26.926513246193025
13	33.98216260680985	17.87753913245406	21.38420847551688	26.756089785219206
14	24.518740676892076	19.307443136421423	22.446501595998	33.7273145906885
15	28.6773879947889	24.756074041712424	19.878342051300216	26.688195912198466
16	26.635651958295455	23.73609159572249	21.780157671220476	27.84809877476158
17	26.79072550015153	23.732155719025634	22.014735922353022	27.46238285846981
18	26.36742196140479	22.247346235137144	23.387176126546308	27.998055676911754
19	28.17162783924305	22.60137834401924	22.970957165853907	26.2560366508838
20	28.677191200954056	21.832504831288645	23.007954406804345	26.482349560952954
21	27.259094827077256	22.94950663785605	21.89292053858537	27.898477996481326
22	27.842194959716306	22.85917826766323	21.450527997858885	27.84809877476158
23	27.460021332451696	22.929236872867246	22.235735398881424	27.375006395799634
24	28.063391230079542	22.21054578802155	22.20169006545363	27.524372916445273
25	28.29304963534102	22.2526596686779	22.499439137570697	26.954851558410375
26	27.9669622510066	22.701939993623878	22.358534751823296	26.972563003546224
27	27.296288861862532	22.20228044695816	23.14905558638659	27.352375104792714
28	26.393398747604035	22.971547547358437	22.58779956941509	28.047254135622442
29	27.021564668422066	23.479275641252713	22.2756845473545	27.22347514297072
30	26.721650864121727	23.29625737484896	23.03432478067327	26.947766980356043
31	27.411413255245538	22.44984709119033	22.7908908069728	27.347848846591333
32	25.682185828482368	22.48664753830592	23.1799522184569	28.651214414754815
33	26.307006254108074	22.054881864660945	23.78568364210286	27.852428239128123
34	26.269418631653107	21.897250002951907	23.840785915858827	27.992545449536156
35	26.27020580699248	22.464606628803534	24.179861693292874	27.085325870911113
36	25.91381217209227	21.720922726932812	24.951096732041577	27.41416836893334
37	26.38296867435737	20.690116620026526	24.891861787753914	28.035052917862192
38	25.19571146875111	20.528352087785795	26.53174481349848	27.744191629964615
39	24.683850704325135	19.548712377938624	27.667638828210787	28.09979808952545
40	24.24854274165299	20.081039701188242	28.60851015259394	27.061907404564828
41	22.95875594809366	20.05663726566774	29.367347179747554	27.617259606491046
42	22.149146111550618	20.33155825294305	29.65505976628764	27.86423586921869
43	21.214768983717278	20.788907125117582	29.850672838121326	28.145651053043807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.0
2	6.0
3	14.0
4	22.0
5	22.0
6	26.5
7	31.0
8	32.5
9	34.0
10	48.0
11	62.0
12	62.0
13	112.5
14	163.0
15	294.5
16	426.0
17	438.0
18	450.0
19	450.0
20	564.0
21	678.0
22	780.5
23	883.0
24	992.5
25	1102.0
26	1102.0
27	1429.0
28	1756.0
29	2674.0
30	3592.0
31	4181.0
32	4770.0
33	4770.0
34	5938.0
35	7106.0
36	7988.0
37	8870.0
38	11633.5
39	14397.0
40	14397.0
41	17004.0
42	19611.0
43	21565.0
44	23519.0
45	28941.0
46	34363.0
47	34363.0
48	38518.0
49	42673.0
50	47102.0
51	51531.0
52	54556.0
53	57581.0
54	57581.0
55	52714.5
56	47848.0
57	45144.5
58	42441.0
59	39806.0
60	37171.0
61	37171.0
62	34243.0
63	31315.0
64	27133.0
65	22951.0
66	20168.0
67	17385.0
68	17385.0
69	14971.5
70	12558.0
71	10812.0
72	9066.0
73	7361.5
74	5657.0
75	5657.0
76	4515.5
77	3374.0
78	2796.5
79	2219.0
80	1707.5
81	1196.0
82	1196.0
83	939.0
84	682.0
85	505.5
86	329.0
87	257.0
88	185.0
89	185.0
90	139.5
91	94.0
92	60.0
93	26.0
94	18.5
95	11.0
96	11.0
97	9.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	508146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.977965100689566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78963270388044	50.318862960072465
2	6.833677929787557	7.924054810451511
3	2.088767909196508	3.633075388285064
4	1.0092830039602299	2.340646991213014
5	0.6295365751029321	1.8249624790462722
6	0.4298053224669276	1.4951542791646892
7	0.29050410782219943	1.1789985917445707
8	0.23276745448503294	1.079630668216767
9	0.18879235262163632	0.9851216788416878
>10	1.2752919180366744	14.468368909205513
>50	0.14679361461729432	5.937414985198681
>100	0.08446593115835564	8.577143700291275
>500	6.811768641802874E-4	0.2365645582684627
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	669	0.13165507550979444	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	529	0.10410393863181054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.9679383484274204E-4	0.0
9	0.0	0.0	0.0	1.9679383484274204E-4	0.0
10	0.0	0.0	0.0	1.9679383484274204E-4	0.0
11	0.0	0.0	0.0	1.9679383484274204E-4	0.0
12	0.0	0.0	0.0	1.9679383484274204E-4	0.0
13	0.0	0.0	0.0	1.9679383484274204E-4	0.0
14	0.0	0.0	0.0	1.9679383484274204E-4	0.0
15	0.0	0.0	0.0	1.9679383484274204E-4	0.0
16	0.0	1.9679383484274204E-4	0.0	1.9679383484274204E-4	0.0
17	0.0	1.9679383484274204E-4	0.0	3.935876696854841E-4	0.0
18	0.0	1.9679383484274204E-4	0.0	7.871753393709682E-4	0.0
19	0.0	1.9679383484274204E-4	0.0	9.839691742137102E-4	0.0
20	0.0	1.9679383484274204E-4	0.0	0.0013775568438991943	0.0
21	0.0	1.9679383484274204E-4	0.0	0.0019679383484274204	0.0
22	0.0	1.9679383484274204E-4	0.0	0.0037390828620120988	0.0
23	0.0	1.9679383484274204E-4	0.0	0.0053134335407540355	0.0
24	0.0	1.9679383484274204E-4	0.0	0.006887784219495972	0.0
25	0.0	1.9679383484274204E-4	0.0	0.00865892873308065	0.0
26	0.0	1.9679383484274204E-4	0.0	0.01259480542993549	0.0
27	0.0	1.9679383484274204E-4	0.0	0.04427861283961696	0.0
28	0.0	1.9679383484274204E-4	0.0	0.15920621238777832	0.0
29	0.0	1.9679383484274204E-4	0.0	0.3253002089950526	0.0
30	0.0	1.9679383484274204E-4	0.0	0.5557457895959036	0.0
31	0.0	1.9679383484274204E-4	0.0	1.2110692596222346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	390	0.0	30.83333	1
GTCTTAC	25	0.005493494	29.599998	1
ATATACA	50	2.6992284E-4	22.199999	4
TCATGAC	50	2.6992284E-4	22.199999	3
ATTGACT	50	2.6992284E-4	22.199999	31
GGATATA	60	3.7210208E-5	21.583334	2
GATATAC	60	3.7210208E-5	21.583334	3
TATACAA	60	3.7210208E-5	21.583334	5
TTCTAGT	45	0.003822915	20.555555	2
TAGATTG	45	0.003822915	20.555555	5
TTTTACG	45	0.003822915	20.555555	4
TCTATCC	45	0.003822915	20.555555	3
ATACGGC	55	5.1376666E-4	20.181818	29
CTTCTAG	65	6.892447E-5	19.923077	1
GGGATAT	65	6.892447E-5	19.923077	1
CGATAGT	140	3.6379788E-12	19.821428	28
AGTCGGT	75	9.2490955E-6	19.733334	11
GTATCAA	610	0.0	19.713116	2
GCGGGTA	180	0.0	19.527777	22
GTTTCGA	95	1.671524E-7	19.473684	15
>>END_MODULE