FastQCFastQC Report
Fri 10 Feb 2017
ERR1632597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21694
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA470.21664976491195723No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT350.16133493131741497TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA320.14750622291877938No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT320.14750622291877938No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA300.13828708398635567No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA260.11984880612150824No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA260.11984880612150824No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA250.1152392366552964No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT240.11062966718908454No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG230.10602009772287269No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.10602009772287269No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT220.10141052825666083No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA220.10141052825666083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCAC358.5903273E-426.4285723
TGTCTCT1701.200533E-915.23529437
CCTGTCT1101.3256835E-515.13636436
CTGTCTC2900.013.39655137
Produced by FastQC (version 0.11.5)