##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21694
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.056882087213054	34.0	33.0	34.0	31.0	34.0
2	33.19821148704711	34.0	33.0	34.0	31.0	34.0
3	33.267124550566976	34.0	34.0	34.0	31.0	34.0
4	36.541716603669215	37.0	37.0	37.0	35.0	37.0
5	36.50419470821425	37.0	37.0	37.0	35.0	37.0
6	36.55831105374758	37.0	37.0	37.0	35.0	37.0
7	36.52876371346916	37.0	37.0	37.0	35.0	37.0
8	36.512676316032085	37.0	37.0	37.0	35.0	37.0
9	38.307596570480314	39.0	39.0	39.0	37.0	39.0
10	38.340693279247716	39.0	39.0	39.0	37.0	39.0
11	38.39112196920808	39.0	39.0	39.0	37.0	39.0
12	38.35885498294459	39.0	39.0	39.0	37.0	39.0
13	38.41315571125657	39.0	39.0	39.0	37.0	39.0
14	39.97976399004333	41.0	40.0	41.0	38.0	41.0
15	39.969899511385634	41.0	40.0	41.0	38.0	41.0
16	39.93975292707661	41.0	40.0	41.0	38.0	41.0
17	39.91868719461602	41.0	40.0	41.0	38.0	41.0
18	39.92827509910575	41.0	40.0	41.0	38.0	41.0
19	39.92435696505947	41.0	40.0	41.0	38.0	41.0
20	39.877569834977415	41.0	40.0	41.0	38.0	41.0
21	39.874942380381675	41.0	40.0	41.0	38.0	41.0
22	39.8469161980271	41.0	40.0	41.0	38.0	41.0
23	39.83193509726192	41.0	40.0	41.0	38.0	41.0
24	39.80284871393012	41.0	40.0	41.0	38.0	41.0
25	39.744214990319904	41.0	40.0	41.0	38.0	41.0
26	39.64487876832304	41.0	40.0	41.0	37.0	41.0
27	39.55997049875542	41.0	40.0	41.0	37.0	41.0
28	39.52562920623214	41.0	40.0	41.0	37.0	41.0
29	39.47796625795151	41.0	40.0	41.0	37.0	41.0
30	39.445975845856	41.0	39.0	41.0	37.0	41.0
31	39.375311145938966	41.0	39.0	41.0	36.0	41.0
32	39.35383055222642	41.0	39.0	41.0	36.0	41.0
33	39.25647644510003	41.0	39.0	41.0	35.0	41.0
34	39.21162533419378	41.0	39.0	41.0	35.0	41.0
35	39.16497649119572	41.0	39.0	41.0	35.0	41.0
36	39.09417350419471	41.0	39.0	41.0	35.0	41.0
37	39.057158661381024	41.0	39.0	41.0	35.0	41.0
38	38.95459574075781	41.0	39.0	41.0	35.0	41.0
39	38.87517285885498	40.0	38.0	41.0	35.0	41.0
40	38.788651239974186	40.0	38.0	41.0	35.0	41.0
41	38.748640177007466	40.0	38.0	41.0	35.0	41.0
42	38.69171199409975	40.0	38.0	41.0	35.0	41.0
43	37.89052272517747	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	3.0
22	3.0
23	4.0
24	13.0
25	19.0
26	25.0
27	30.0
28	51.0
29	58.0
30	101.0
31	122.0
32	178.0
33	238.0
34	352.0
35	539.0
36	866.0
37	1586.0
38	4143.0
39	13361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56965059463446	16.064349589748318	12.464275836636858	27.901723978980364
2	21.858578408776623	18.60883193509726	30.556835991518394	28.975753664607723
3	21.80787314464829	18.95454964506315	27.413109615561908	31.824467594726652
4	16.80649027380843	14.533972526965982	31.78759103899696	36.87194616022864
5	18.069512307550475	32.386835069604494	32.34534894440859	17.19830367843643
6	37.76620263667373	31.889001567253615	13.865584954365263	16.479210841707385
7	31.211394855720474	26.97981008573799	19.406287452751915	22.40250760578962
8	28.795980455425465	30.072831197566146	18.774776435880888	22.3564119111275
9	27.68968378353462	12.680925601548815	16.930948649396147	42.69844196552042
10	19.87185396883931	23.282935373836082	28.745275191297132	28.09993546602747
11	41.029777818751725	18.415230017516365	17.90817737623306	22.646814787498847
12	23.398174610491377	22.771273163086565	26.265326818475152	27.565225407946897
13	35.87627915552687	16.631326634092375	19.433944869549183	28.058449340831565
14	25.200516271780216	18.346086475523187	22.268830091269475	34.18456716142712
15	30.086659905964787	23.30137365170093	18.996035770259056	27.61593067207523
16	27.39928090716327	22.706739190559603	21.019636765926062	28.874343136351065
17	27.768046464460223	22.453212869917948	21.047294182723334	28.731446482898498
18	27.731169908730525	21.001198488061217	22.370240619526136	28.897390983682126
19	28.777542177560615	21.595832949202546	22.411726744722042	27.214898128514797
20	28.96192495620909	21.572785101871485	21.964598506499495	27.50069143541993
21	28.602378537844565	21.53129897667558	21.153314280446207	28.713008205033653
22	28.874343136351065	21.568175532405274	20.452659721582002	29.104821609661656
23	29.150917304323777	21.222457822439385	21.761777449986173	27.864847423250666
24	29.05872591499954	20.775329584216834	20.936664515534247	29.229279985249377
25	29.160136443256203	21.715681755324052	21.522079837743156	27.602101963676596
26	28.749884760763344	22.098276021019636	21.065732460588183	28.086106757628837
27	27.371623490365998	21.761777449986173	23.250668387572603	27.61593067207523
28	27.961648382041115	21.51747026827694	21.379183184290586	29.14169816539135
29	28.288927814142163	22.37945975845856	21.41145017055407	27.92016225684521
30	27.906333548446575	21.53590854614179	22.729787037890663	27.827970867520975
31	28.33041393933807	21.328477920162257	21.63270950493224	28.708398635567438
32	27.417719185028115	21.397621462155435	22.075228173688576	29.10943117912787
33	26.75855075135982	21.545127685074213	23.034018622660643	28.66230294090532
34	26.859961279616485	21.34230662856089	22.812759288282475	28.98497280354015
35	26.560339264312717	22.144371715681753	24.006637780031344	27.288651239974186
36	26.717064626163918	21.282382225500136	23.49036599981562	28.510187148520327
37	27.69429335300083	20.355858762791556	23.499585138748042	28.450262745459575
38	26.41744261086015	20.31437263759565	25.043790909929015	28.224393841615193
39	25.78593159398912	19.581451092467965	26.196183276481978	28.436434037060938
40	25.513966995482622	20.5678989582373	26.67557850096801	27.24255554531207
41	23.780768876186965	20.166866414676868	27.380842629298424	28.671522079837743
42	23.038628192126858	19.954826219231123	29.307642666174978	27.698902922467038
43	22.42094588365447	20.46648842998064	28.109154604959897	29.003411081404995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	5.5
14	9.0
15	14.0
16	19.0
17	14.5
18	10.0
19	10.0
20	18.5
21	27.0
22	19.0
23	11.0
24	24.5
25	38.0
26	38.0
27	46.0
28	54.0
29	65.0
30	76.0
31	95.0
32	114.0
33	114.0
34	157.0
35	200.0
36	226.5
37	253.0
38	321.5
39	390.0
40	390.0
41	508.5
42	627.0
43	710.0
44	793.0
45	1046.5
46	1300.0
47	1300.0
48	1463.0
49	1626.0
50	1836.0
51	2046.0
52	2125.0
53	2204.0
54	2204.0
55	2178.5
56	2153.0
57	2169.5
58	2186.0
59	2101.0
60	2016.0
61	2016.0
62	1818.5
63	1621.0
64	1411.5
65	1202.0
66	1084.0
67	966.0
68	966.0
69	802.5
70	639.0
71	551.5
72	464.0
73	353.5
74	243.0
75	243.0
76	207.0
77	171.0
78	138.5
79	106.0
80	90.0
81	74.0
82	74.0
83	49.0
84	24.0
85	21.5
86	19.0
87	13.0
88	7.0
89	7.0
90	4.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	21694.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.57177099658891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.89802147490053	78.56550198211487
2	4.229574317327083	7.154051811560801
3	1.0900964735379082	2.7657416797271135
4	0.5723006486074018	1.9360191758089795
5	0.2943260478552352	1.244583755877201
6	0.2452717065460293	1.244583755877201
7	0.12536109445685945	0.7421406840601088
8	0.07085627077996402	0.47939522448603294
9	0.06540578841227448	0.4978335023508805
>10	0.40878617757671554	5.370148428136812
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	47	0.21664976491195723	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	35	0.16133493131741497	TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	32	0.14750622291877938	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	32	0.14750622291877938	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	30	0.13828708398635567	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	26	0.11984880612150824	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	26	0.11984880612150824	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	25	0.1152392366552964	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	24	0.11062966718908454	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	23	0.10602009772287269	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	23	0.10602009772287269	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	22	0.10141052825666083	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	22	0.10141052825666083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.004609569466211856	0.0
27	0.0	0.0	0.0	0.027657416797271135	0.0
28	0.0	0.0	0.0	0.10602009772287269	0.0
29	0.0	0.0	0.0	0.2489167511754402	0.0
30	0.0	0.0	0.0	0.3779846962293722	0.0
31	0.0	0.0	0.0	0.7882363787222273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGCAC	35	8.5903273E-4	26.428572	3
TGTCTCT	170	1.200533E-9	15.235294	37
CCTGTCT	110	1.3256835E-5	15.136364	36
CTGTCTC	290	0.0	13.396551	37
>>END_MODULE