FastQCFastQC Report
Fri 10 Feb 2017
ERR1632596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA160.45236075770426915No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT150.4240882103477523RNA PCR Primer, Index 39 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA130.3675431156347187No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA130.3675431156347187No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA120.33927056827820185No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA120.33927056827820185No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG110.31099802092168505No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG110.31099802092168505No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC100.28272547356516825No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT90.2544529262086514No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT90.2544529262086514No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC80.22618037885213457RNA PCR Primer, Index 39 (95% over 21bp)
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC80.22618037885213457No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT80.22618037885213457No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC70.19790783149561775No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC70.19790783149561775RNA PCR Primer, Index 39 (95% over 24bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA70.19790783149561775No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC70.19790783149561775No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG70.19790783149561775No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC70.19790783149561775No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA70.19790783149561775No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA70.19790783149561775No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT70.19790783149561775No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA60.16963528413910092No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC60.16963528413910092No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT60.16963528413910092No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC60.16963528413910092No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.16963528413910092No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC60.16963528413910092No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT50.14136273678258413No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC50.14136273678258413No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA50.14136273678258413No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT50.14136273678258413No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC50.14136273678258413No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT50.14136273678258413No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA50.14136273678258413No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAG50.14136273678258413No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT50.14136273678258413No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT50.14136273678258413No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG50.14136273678258413No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT50.14136273678258413No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA50.14136273678258413No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA40.11309018942606729No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA40.11309018942606729No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACT40.11309018942606729No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG40.11309018942606729No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT40.11309018942606729No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA40.11309018942606729No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTT40.11309018942606729No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA40.11309018942606729No Hit
GAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT40.11309018942606729No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT40.11309018942606729No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC40.11309018942606729No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT40.11309018942606729No Hit
ATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTA40.11309018942606729No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA40.11309018942606729No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGG40.11309018942606729No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG40.11309018942606729No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC40.11309018942606729No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT40.11309018942606729No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTG40.11309018942606729No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG40.11309018942606729No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG40.11309018942606729No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA40.11309018942606729No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTT40.11309018942606729No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT40.11309018942606729No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG40.11309018942606729No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC40.11309018942606729No Hit
TTCTCTACCTGACCACCTGTGTCGGTTTGGGGTACGATTTGAT40.11309018942606729No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA40.11309018942606729No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC40.11309018942606729No Hit
CACTAAAGATGACGGAGTGGACGCCCGTACTCTGCGTTGATAC40.11309018942606729No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)