FastQCFastQC Report
Fri 10 Feb 2017
ERR1632595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225391
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5540.24579508498564717No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4250.18856121140595677No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3110.1379824394053001No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2650.11757346122959658No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2480.1100310127733583No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2440.1082563190189493No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2410.10692529870314255No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2360.10470693151013127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA303.5903833E-430.83333224
TATACGG303.5903833E-430.8333325
TACGGTG250.00548708829.5999987
ATACGGT250.00548708829.5999986
CCTGACG250.00548708829.59999814
ACGGTGT250.00548708829.5999988
GGTATCA2200.029.4318181
AAGACTT405.9192826E-527.755
GCGAACT400.00192639923.12523
GAACTAC400.00192639923.12525
AGCGAGA400.00192639923.12524
TAAGACT400.00192639923.1254
TCACGTA400.00192639923.12525
TTCGCTT502.6927728E-422.19999910
CTTATAC2400.021.58333237
TGCACCG603.709203E-521.5833325
AGACTTC450.003816412820.5555576
TAGAGAT450.003816412820.5555574
ATTACTC450.003816412820.5555573
GGATCTT450.003816412820.5555576
Produced by FastQC (version 0.11.5)