##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632595.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225391
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03534302611906	34.0	33.0	34.0	31.0	34.0
2	33.17314799614891	34.0	33.0	34.0	31.0	34.0
3	33.24943764391657	34.0	34.0	34.0	31.0	34.0
4	36.53649879542662	37.0	37.0	37.0	35.0	37.0
5	36.49786371239313	37.0	37.0	37.0	35.0	37.0
6	36.5389478728077	37.0	37.0	37.0	35.0	37.0
7	36.534400220062025	37.0	37.0	37.0	35.0	37.0
8	36.509314923843455	37.0	37.0	37.0	35.0	37.0
9	38.29467458771646	39.0	39.0	39.0	37.0	39.0
10	38.32365977346034	39.0	39.0	39.0	37.0	39.0
11	38.389953458656294	39.0	39.0	39.0	37.0	39.0
12	38.353829567285295	39.0	39.0	39.0	37.0	39.0
13	38.383165255045675	39.0	39.0	39.0	37.0	39.0
14	39.945574579286664	41.0	40.0	41.0	38.0	41.0
15	39.937739306360946	41.0	40.0	41.0	38.0	41.0
16	39.90884729203917	41.0	40.0	41.0	38.0	41.0
17	39.90314165161874	41.0	40.0	41.0	38.0	41.0
18	39.88942326889716	41.0	40.0	41.0	38.0	41.0
19	39.89414395428389	41.0	40.0	41.0	38.0	41.0
20	39.85746103438026	41.0	40.0	41.0	38.0	41.0
21	39.838387513254744	41.0	40.0	41.0	38.0	41.0
22	39.80502770740624	41.0	40.0	41.0	38.0	41.0
23	39.764418277570975	41.0	40.0	41.0	38.0	41.0
24	39.73884937730433	41.0	40.0	41.0	38.0	41.0
25	39.69729492304484	41.0	40.0	41.0	37.0	41.0
26	39.609460892404755	41.0	40.0	41.0	37.0	41.0
27	39.517123576362856	41.0	40.0	41.0	37.0	41.0
28	39.48607087239508	41.0	40.0	41.0	37.0	41.0
29	39.43840703488604	41.0	39.0	41.0	37.0	41.0
30	39.40281555164137	41.0	39.0	41.0	37.0	41.0
31	39.334889148191365	41.0	39.0	41.0	36.0	41.0
32	39.26935858130981	41.0	39.0	41.0	36.0	41.0
33	39.212714793403464	41.0	39.0	41.0	36.0	41.0
34	39.15849346247188	41.0	39.0	41.0	35.0	41.0
35	39.07581048045397	41.0	39.0	41.0	35.0	41.0
36	39.03132334476532	41.0	39.0	41.0	35.0	41.0
37	38.975389434360736	40.0	39.0	41.0	35.0	41.0
38	38.88968059949155	40.0	39.0	41.0	35.0	41.0
39	38.79546654480436	40.0	38.0	41.0	35.0	41.0
40	38.721355333620245	40.0	38.0	41.0	35.0	41.0
41	38.66474703958898	40.0	38.0	41.0	35.0	41.0
42	38.596172872918615	40.0	38.0	41.0	35.0	41.0
43	37.84015333354038	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	1.0
19	7.0
20	14.0
21	30.0
22	47.0
23	88.0
24	152.0
25	201.0
26	283.0
27	414.0
28	606.0
29	780.0
30	1035.0
31	1334.0
32	1870.0
33	2328.0
34	3506.0
35	5462.0
36	8973.0
37	17225.0
38	45714.0
39	135318.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.36331086866822	16.23756050596519	12.306613839949243	28.092514785417343
2	21.577170339543283	19.212390911793285	31.64988841613019	27.56055033253324
3	21.65348217098287	19.253208868144693	28.052140502504535	31.041168458367903
4	16.406600086072647	15.044966303002338	32.266594495787324	36.28183911513769
5	17.750043258160265	32.885962616076064	32.275911637997964	17.088082487765703
6	36.31511462303286	32.616652838844495	14.535185522048351	16.53304701607429
7	31.475968428198108	26.849341810453836	18.91867909543859	22.756010665909464
8	27.869347045800406	30.58374114316898	19.534054154779916	22.01285765625069
9	28.069443766610025	12.799978703674947	16.883105359131463	42.24747217058356
10	19.547364357937983	23.348314706443468	29.220332666344262	27.883988269274283
11	39.8897915178512	19.24300437905684	18.446610556765798	22.420593546326163
12	23.40554858002316	22.919726164753694	26.346216131078883	27.32850912414426
13	35.24453061568563	17.09473758934474	20.508361025950457	27.152370769019168
14	24.841275827340045	18.802436654524804	22.001322146847034	34.35496537128812
15	29.38271714487269	23.714345293290325	19.399621102883433	27.503316458953552
16	27.041008735930006	23.279988996898723	21.34646015147011	28.33254211570116
17	27.820099294115558	22.90464126784122	21.181413632310075	28.093845805733146
18	26.839137321365982	21.381066679681087	22.986720853982632	28.7930751449703
19	29.039757576833146	21.96849031239047	22.237800089622034	26.753952021154348
20	29.792227728702564	20.592658979284888	22.107803772111577	27.507309519900975
21	28.011322546153128	21.773717672844082	21.341579743645486	28.873380037357304
22	28.490933533282163	22.35315518365862	20.72265529679535	28.433255986263873
23	28.726967802618557	21.981800515548535	21.382397699996893	27.90883398183601
24	28.625366585178647	21.49997116122649	21.480005856489388	28.39465639710547
25	29.3059616399945	21.561641769192203	21.71692747270299	27.41546911811031
26	28.749151474548672	22.235581722429025	21.563860136385216	27.45140666663709
27	27.976716017942156	21.645496049088027	22.689015976680523	27.688771956289294
28	27.399053200882022	22.42369926039638	21.56696585045543	28.61028168826617
29	27.67590542656983	22.99781268994769	21.59048054270135	27.73580134078113
30	27.667031957797782	21.90903807161777	23.012897586860166	27.411032383724283
31	28.199883757559085	22.062549081374144	21.81897236358151	27.91859479748526
32	25.989059013004066	21.900608276284323	22.620246593697175	29.49008611701443
33	27.052544245333664	21.605121766175227	23.215212674862794	28.12712131362832
34	27.13728587210669	21.447174022032822	23.030200850965656	28.385339254894827
35	26.700267535083476	22.411276404115515	23.50404408339286	27.384411977408146
36	26.770367938382634	21.226668323047505	24.45572360919469	27.547240129375172
37	27.2765993318278	20.574024694863592	23.89669507655585	28.252680896752754
38	25.870598204897266	20.440478989844316	25.67449454503507	28.014428260223344
39	25.579992102612792	19.792272096046425	26.282327155920154	28.345408645420623
40	24.99789255116664	20.238163901841688	27.533929926217105	27.230013620774564
41	23.516466939673723	19.798039850748257	28.359162522017296	28.32633068756073
42	22.6255706749604	20.368603892790752	28.993615539218514	28.01220989303033
43	21.83893766831861	20.724429990549755	28.75669392300491	28.67993841812672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	4.5
4	6.0
5	6.0
6	9.0
7	12.0
8	9.5
9	7.0
10	15.5
11	24.0
12	24.0
13	39.5
14	55.0
15	92.0
16	129.0
17	135.0
18	141.0
19	141.0
20	177.0
21	213.0
22	230.0
23	247.0
24	310.5
25	374.0
26	374.0
27	475.5
28	577.0
29	814.0
30	1051.0
31	1253.5
32	1456.0
33	1456.0
34	1913.5
35	2371.0
36	2776.5
37	3182.0
38	4164.0
39	5146.0
40	5146.0
41	6158.5
42	7171.0
43	8100.5
44	9030.0
45	11265.0
46	13500.0
47	13500.0
48	15349.0
49	17198.0
50	19572.0
51	21946.0
52	23671.0
53	25396.0
54	25396.0
55	23828.5
56	22261.0
57	21694.0
58	21127.0
59	19951.5
60	18776.0
61	18776.0
62	17617.5
63	16459.0
64	14053.0
65	11647.0
66	10178.5
67	8710.0
68	8710.0
69	7453.5
70	6197.0
71	5354.0
72	4511.0
73	3595.0
74	2679.0
75	2679.0
76	2160.5
77	1642.0
78	1333.0
79	1024.0
80	784.0
81	544.0
82	544.0
83	424.0
84	304.0
85	222.0
86	140.0
87	111.0
88	82.0
89	82.0
90	57.5
91	33.0
92	22.0
93	11.0
94	9.0
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	225391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.772738367300356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.14914412453243	44.71157172473432
2	8.248325843577044	8.870701351098207
3	2.6064477035884273	4.20467491299334
4	1.3074218486572002	2.8121461201414317
5	0.9115015499321351	2.450696718294473
6	0.5833357127919232	1.8820535198557653
7	0.46103390840624275	1.735373901462797
8	0.36380215538881333	1.5650110495346086
9	0.2606933884351111	1.261637763336561
>10	1.8882464391736304	19.279070976506635
>50	0.16057507537728435	5.762770315875255
>100	0.058481045970739366	5.169010962204792
>500	9.912041689955826E-4	0.29528068396179163
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	554	0.24579508498564717	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	425	0.18856121140595677	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	311	0.1379824394053001	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	265	0.11757346122959658	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	248	0.1100310127733583	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	244	0.1082563190189493	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	241	0.10692529870314255	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	236	0.10470693151013127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	4.436734386022512E-4	0.0
17	0.0	0.0	0.0	8.873468772045024E-4	0.0
18	0.0	0.0	0.0	8.873468772045024E-4	0.0
19	0.0	0.0	0.0	0.0013310203158067536	0.0
20	0.0	0.0	0.0	0.0017746937544090048	0.0
21	0.0	0.0	0.0	0.002218367193011256	0.0
22	0.0	0.0	0.0	0.003993060947420261	0.0
23	0.0	0.0	0.0	0.004436734386022512	0.0
24	0.0	0.0	0.0	0.0053240812632270144	0.0
25	0.0	0.0	0.0	0.0053240812632270144	0.0
26	0.0	0.0	0.0	0.006211428140431517	0.0
27	0.0	0.0	0.0	0.027951426631941827	0.0
28	0.0	0.0	0.0	0.13842611284390238	0.0
29	0.0	0.0	0.0	0.2799579397580205	0.0
30	0.0	0.0	0.0	0.4765052730588178	0.0
31	0.0	0.0	0.0	1.0098007462587237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	30	3.5903833E-4	30.833332	24
TATACGG	30	3.5903833E-4	30.833332	5
TACGGTG	25	0.005487088	29.599998	7
ATACGGT	25	0.005487088	29.599998	6
CCTGACG	25	0.005487088	29.599998	14
ACGGTGT	25	0.005487088	29.599998	8
GGTATCA	220	0.0	29.431818	1
AAGACTT	40	5.9192826E-5	27.75	5
GCGAACT	40	0.001926399	23.125	23
GAACTAC	40	0.001926399	23.125	25
AGCGAGA	40	0.001926399	23.125	24
TAAGACT	40	0.001926399	23.125	4
TCACGTA	40	0.001926399	23.125	25
TTCGCTT	50	2.6927728E-4	22.199999	10
CTTATAC	240	0.0	21.583332	37
TGCACCG	60	3.709203E-5	21.583332	5
AGACTTC	45	0.0038164128	20.555557	6
TAGAGAT	45	0.0038164128	20.555557	4
ATTACTC	45	0.0038164128	20.555557	3
GGATCTT	45	0.0038164128	20.555557	6
>>END_MODULE