FastQCFastQC Report
Fri 10 Feb 2017
ERR1632594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23973
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA600.25028156676260793No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA590.24611020731656447No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT440.18353981562591248TruSeq Adapter, Index 1 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA360.15016894005756476No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC330.13765486171943436No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA330.13765486171943436No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT320.1334835022733909No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA300.12514078338130397No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA290.12096942393526051No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC280.11679806448921703No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT260.10845534559713012No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC260.10845534559713012No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC250.10428398615108664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCCG250.00539088729.5999985
TAACTCC250.00539088729.5999984
GCTAACT250.00539088729.5999982
CTCTTAT656.5487504E-519.92307737
ACTCCGT500.006840566618.4999986
TCTCTTA1102.3028433E-413.45454537
CTGTCTC2356.348273E-1012.59574537
GTCTCTT1256.775962E-411.8436
TGTCTCT1500.00310501859.86666735
Produced by FastQC (version 0.11.5)