##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632594.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23973
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92954573895633	34.0	31.0	34.0	31.0	34.0
2	33.07145538731073	34.0	33.0	34.0	31.0	34.0
3	33.153798022775625	34.0	33.0	34.0	31.0	34.0
4	36.48934217661536	37.0	37.0	37.0	35.0	37.0
5	36.416301672715136	37.0	37.0	37.0	35.0	37.0
6	36.45622158261377	37.0	37.0	37.0	35.0	37.0
7	36.460434655654275	37.0	37.0	37.0	35.0	37.0
8	36.44424978100363	37.0	37.0	37.0	35.0	37.0
9	38.1934676511075	39.0	39.0	39.0	37.0	39.0
10	38.21194677345347	39.0	38.0	39.0	37.0	39.0
11	38.266341300629875	39.0	39.0	39.0	37.0	39.0
12	38.24285654694865	39.0	39.0	39.0	37.0	39.0
13	38.26759270846369	39.0	39.0	39.0	37.0	39.0
14	39.779752221248906	41.0	40.0	41.0	38.0	41.0
15	39.76623701664372	41.0	40.0	41.0	38.0	41.0
16	39.752638384849625	41.0	40.0	41.0	38.0	41.0
17	39.73582780628207	41.0	40.0	41.0	38.0	41.0
18	39.74421223876861	41.0	40.0	41.0	38.0	41.0
19	39.72986276227422	41.0	40.0	41.0	38.0	41.0
20	39.69453134776624	41.0	40.0	41.0	38.0	41.0
21	39.70692028532098	41.0	40.0	41.0	38.0	41.0
22	39.6637884286489	41.0	40.0	41.0	37.0	41.0
23	39.59612897843407	41.0	40.0	41.0	37.0	41.0
24	39.60543110999875	41.0	40.0	41.0	37.0	41.0
25	39.54623951945939	41.0	40.0	41.0	37.0	41.0
26	39.48746506486464	41.0	39.0	41.0	37.0	41.0
27	39.402327618570894	41.0	39.0	41.0	37.0	41.0
28	39.339632086096856	41.0	39.0	41.0	36.0	41.0
29	39.28849121928837	41.0	39.0	41.0	36.0	41.0
30	39.27201434947649	40.0	39.0	41.0	36.0	41.0
31	39.18725232553289	40.0	39.0	41.0	36.0	41.0
32	39.11809118591749	40.0	39.0	41.0	35.0	41.0
33	39.062945814040795	40.0	39.0	41.0	35.0	41.0
34	39.03649939515288	40.0	39.0	41.0	35.0	41.0
35	38.95311391982647	40.0	39.0	41.0	35.0	41.0
36	38.90134734910107	40.0	39.0	41.0	35.0	41.0
37	38.82872398114546	40.0	38.0	41.0	35.0	41.0
38	38.73883118508322	40.0	38.0	41.0	35.0	41.0
39	38.68827430859717	40.0	38.0	41.0	35.0	41.0
40	38.58853710424227	40.0	38.0	41.0	35.0	41.0
41	38.52016852292162	40.0	38.0	41.0	35.0	41.0
42	38.426187794602264	40.0	38.0	41.0	35.0	41.0
43	37.681141283944434	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	7.0
23	5.0
24	20.0
25	24.0
26	35.0
27	40.0
28	61.0
29	85.0
30	113.0
31	172.0
32	221.0
33	302.0
34	383.0
35	686.0
36	1034.0
37	2166.0
38	5681.0
39	12936.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.127685312643386	16.95657614816669	12.305510365828223	27.6102281733617
2	21.415759395987152	19.371793267425854	31.180911859174902	28.03153547741209
3	21.140449672548282	19.809786009260417	28.565469486505652	30.48429483168565
4	16.10561882117382	15.529971217619822	32.98293913986568	35.38147082134068
5	17.594794143411338	32.4573478496642	32.649230384182204	17.298627622742252
6	35.6234096692112	33.37087556834773	14.69569932841113	16.31001543402995
7	31.2476536103116	27.063780085930006	19.238309765152465	22.45025653860593
8	28.119134025779	30.471780753347517	19.200767530138073	22.20831769073541
9	27.54765778167105	12.69761815375631	17.023317899303382	42.731406165269256
10	19.046427230634464	23.388812413965713	29.97538897926834	27.58937137613148
11	40.624869645017306	18.925457806699203	18.303925249238727	22.145747299044757
12	23.463896883994494	23.06344637717432	25.97088391106662	27.50177282776457
13	35.4607266508155	17.56142326784299	20.289492345555416	26.688357735786095
14	24.93638676844784	18.854544696116466	22.258374004087933	33.950694531347764
15	29.086889417261087	24.089600800901014	19.697159304217244	27.126350477620658
16	27.455887873858092	22.888249280440494	21.84958077837567	27.806282067325743
17	27.28903349601635	23.15938764443332	21.60347057105911	27.94810828849122
18	27.059608726483965	21.520043382138237	22.496141492512407	28.924206398865387
19	28.62386851875026	21.903808451174235	23.055103658282235	26.417219371793266
20	29.762649647520128	20.685771492929543	22.037291953447628	27.514286906102697
21	28.098277228548785	21.62015600884328	21.599299211613065	28.682267550994865
22	28.548784048721476	22.70470946481458	20.852625870771284	27.893880615692655
23	29.074375338922952	21.870437575605887	21.177991907562674	27.877195177908483
24	27.881366537354523	21.878780294497975	21.882951653944023	28.356901514203482
25	28.882492804404958	21.67855504108789	22.045634672339716	27.39331748216744
26	29.028490385016475	22.06232011012389	21.586785133274937	27.322404371584703
27	27.17640679097318	22.07483418846202	23.05093229883619	27.697826721728614
28	26.692529095232135	22.746423059275017	21.812038543361282	28.74900930213157
29	27.510115546656657	23.23030075501606	21.732782713886458	27.52680098444083
30	27.39748884161348	21.636841446627457	22.946648312685102	28.019021399073957
31	27.943936929045176	22.412714303591542	21.991406999541148	27.651941767822137
32	26.19196596170692	22.15826137738289	22.66299587035415	28.98677679055604
33	26.150252367246484	21.611813289951197	23.85600467192258	28.381929670879742
34	26.663329579109835	22.09986234513828	23.138530847203103	28.098277228548785
35	26.35047762065657	22.996704626037626	23.755892045217536	26.896925708088265
36	26.90109706753431	21.736954073332498	23.77257748300171	27.58937137613148
37	26.78012764359905	21.5742710549368	24.260626538188795	27.38497476327535
38	26.00008342718892	21.152963750886414	24.873816376757183	27.97313644516748
39	25.10741250573562	20.781712760188544	26.183623242814836	27.927251491261
40	25.758144579318397	20.84845451132524	26.725899970800484	26.667500938555875
41	23.96446001751971	20.42297584782881	27.28486213657031	28.327701998081174
42	22.42522838192967	21.54924289826054	28.686438910440913	27.339089809368872
43	22.542026446418888	21.02782296750511	28.406957827556	28.02319275852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.5
4	2.0
5	2.0
6	1.5
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	6.0
14	11.0
15	13.5
16	16.0
17	22.5
18	29.0
19	29.0
20	29.5
21	30.0
22	25.0
23	20.0
24	35.0
25	50.0
26	50.0
27	53.5
28	57.0
29	82.0
30	107.0
31	138.5
32	170.0
33	170.0
34	216.0
35	262.0
36	319.5
37	377.0
38	459.5
39	542.0
40	542.0
41	663.0
42	784.0
43	903.5
44	1023.0
45	1270.5
46	1518.0
47	1518.0
48	1747.0
49	1976.0
50	2195.0
51	2414.0
52	2441.0
53	2468.0
54	2468.0
55	2416.0
56	2364.0
57	2258.5
58	2153.0
59	2093.5
60	2034.0
61	2034.0
62	1896.5
63	1759.0
64	1471.5
65	1184.0
66	1048.5
67	913.0
68	913.0
69	779.5
70	646.0
71	545.5
72	445.0
73	344.5
74	244.0
75	244.0
76	203.0
77	162.0
78	132.5
79	103.0
80	76.0
81	49.0
82	49.0
83	40.0
84	31.0
85	21.5
86	12.0
87	10.0
88	8.0
89	8.0
90	5.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23973.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.40825094898427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.87333469973356	74.79247486755933
2	4.503996720639476	7.333249906144412
3	1.4193482270957163	3.46639969966212
4	0.6814921090387375	2.2191632252951234
5	0.37405205984832957	1.522546197805865
6	0.22545603607296577	1.101238893755475
7	0.21008403361344538	1.1971801610144746
8	0.11785201885632302	0.7675301380719977
9	0.0819840131174421	0.600675760230259
>10	0.5021520803443328	6.503149376381763
>50	0.010248001639680263	0.4963917740791724
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	60	0.25028156676260793	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	59	0.24611020731656447	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	44	0.18353981562591248	TruSeq Adapter, Index 1 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	36	0.15016894005756476	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	33	0.13765486171943436	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	33	0.13765486171943436	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	32	0.1334835022733909	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	30	0.12514078338130397	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	29	0.12096942393526051	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	28	0.11679806448921703	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	26	0.10845534559713012	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	26	0.10845534559713012	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	25	0.10428398615108664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0041713594460434655	0.0
21	0.0	0.0	0.0	0.0041713594460434655	0.0
22	0.0	0.0	0.0	0.0041713594460434655	0.0
23	0.0	0.0	0.0	0.0041713594460434655	0.0
24	0.0	0.0	0.0	0.0041713594460434655	0.0
25	0.0	0.0	0.0	0.0041713594460434655	0.0
26	0.0	0.0	0.0	0.0041713594460434655	0.0
27	0.0	0.0	0.0	0.016685437784173862	0.0
28	0.0	0.0	0.0	0.07925582947482585	0.0
29	0.0	0.0	0.0	0.17936845617986902	0.0
30	0.0	0.0	0.0	0.36707963125182497	0.0
31	0.0	0.0	0.0	0.7466733408417804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTCCG	25	0.005390887	29.599998	5
TAACTCC	25	0.005390887	29.599998	4
GCTAACT	25	0.005390887	29.599998	2
CTCTTAT	65	6.5487504E-5	19.923077	37
ACTCCGT	50	0.0068405666	18.499998	6
TCTCTTA	110	2.3028433E-4	13.454545	37
CTGTCTC	235	6.348273E-10	12.595745	37
GTCTCTT	125	6.775962E-4	11.84	36
TGTCTCT	150	0.0031050185	9.866667	35
>>END_MODULE