FastQCFastQC Report
Fri 10 Feb 2017
ERR1632593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9108
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT600.6587615283267457Illumina PCR Primer Index 11 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA530.5819060166886254No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA400.4391743522178305No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA390.4281949934123847No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA330.36231884057971014No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG320.3513394817742644No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA310.3403601229688186No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT270.2964426877470355No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC270.2964426877470355No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA270.2964426877470355No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT260.2854633289415898No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT250.27448397013614406No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.2635046113306983No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT230.25252525252525254No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA220.24154589371980675No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC220.24154589371980675No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC200.21958717610891526Illumina PCR Primer Index 11 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA200.21958717610891526No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC200.21958717610891526No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA200.21958717610891526No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG190.2086078173034695No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA190.2086078173034695No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA190.2086078173034695No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC190.2086078173034695No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA190.2086078173034695No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT190.2086078173034695No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC180.1976284584980237No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT180.1976284584980237No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA180.1976284584980237No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT180.1976284584980237No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA180.1976284584980237No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC170.18664909969257795No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT170.18664909969257795No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC170.18664909969257795No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG170.18664909969257795No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA170.18664909969257795No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA160.1756697408871322No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA150.16469038208168643No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC150.16469038208168643No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC150.16469038208168643No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC150.16469038208168643No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC150.16469038208168643No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC140.15371102327624067No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT140.15371102327624067No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC140.15371102327624067No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC140.15371102327624067No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA140.15371102327624067No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA140.15371102327624067No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC140.15371102327624067No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT140.15371102327624067No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA140.15371102327624067No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT130.1427316644707949No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG130.1427316644707949No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA130.1427316644707949No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT130.1427316644707949No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC130.1427316644707949No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG130.1427316644707949No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC130.1427316644707949No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG130.1427316644707949No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC120.13175230566534915No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT120.13175230566534915No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG120.13175230566534915No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG120.13175230566534915No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG120.13175230566534915No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA120.13175230566534915No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC120.13175230566534915No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT120.13175230566534915No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG120.13175230566534915No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG120.13175230566534915No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT110.12077294685990338No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA110.12077294685990338No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA110.12077294685990338No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC110.12077294685990338No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG110.12077294685990338No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT100.10979358805445763No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG100.10979358805445763No Hit
GTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTT100.10979358805445763No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACC100.10979358805445763No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT100.10979358805445763No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT100.10979358805445763No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA100.10979358805445763No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT100.10979358805445763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100.10979358805445763No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT100.10979358805445763No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT100.10979358805445763No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG100.10979358805445763No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT100.10979358805445763No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC100.10979358805445763No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG100.10979358805445763No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG100.10979358805445763No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC100.10979358805445763No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers