FastQCFastQC Report
Fri 10 Feb 2017
ERR1632592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA290.340215861098076No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT280.328484279680901RNA PCR Primer, Index 46 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA210.24636320976067572No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT200.2346316283435007No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA190.22290004692632567No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC160.18770530267480057No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA160.18770530267480057No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA150.17597372125762553No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT140.1642421398404505No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA140.1642421398404505No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC130.15251055842327546No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC120.14077897700610043RNA PCR Primer, Index 46 (95% over 22bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC120.14077897700610043No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC120.14077897700610043No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC120.14077897700610043No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG120.14077897700610043No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT120.14077897700610043No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA120.14077897700610043No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA120.14077897700610043No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC110.1290473955889254No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC110.1290473955889254No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA110.1290473955889254No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC110.1290473955889254No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT100.11731581417175035No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG100.11731581417175035No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA100.11731581417175035No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA90.1055842327545753No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC90.1055842327545753No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC90.1055842327545753No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT90.1055842327545753No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC90.1055842327545753No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA90.1055842327545753No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG90.1055842327545753No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC90.1055842327545753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA400.001778827623.12537
CTCTTAT500.006489948418.49999836
TCTCTTA500.006489948418.49999835
Produced by FastQC (version 0.11.5)