##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632592.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8524
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05302674800563	34.0	33.0	34.0	31.0	34.0
2	33.16400750821211	34.0	33.0	34.0	31.0	34.0
3	33.23392773345847	34.0	34.0	34.0	31.0	34.0
4	36.51947442515251	37.0	37.0	37.0	35.0	37.0
5	36.47524636320976	37.0	37.0	37.0	35.0	37.0
6	36.51748005631159	37.0	37.0	37.0	35.0	37.0
7	36.53026748005631	37.0	37.0	37.0	35.0	37.0
8	36.5161895823557	37.0	37.0	37.0	35.0	37.0
9	38.28120600656968	39.0	39.0	39.0	37.0	39.0
10	38.32836696386673	39.0	39.0	39.0	37.0	39.0
11	38.4015720319099	39.0	39.0	39.0	37.0	39.0
12	38.346198967620836	39.0	39.0	39.0	37.0	39.0
13	38.39030971374941	39.0	39.0	39.0	37.0	39.0
14	39.93137024870953	41.0	40.0	41.0	38.0	41.0
15	39.970788362271236	41.0	40.0	41.0	38.0	41.0
16	39.874354763022055	41.0	40.0	41.0	38.0	41.0
17	39.888901923979354	41.0	40.0	41.0	38.0	41.0
18	39.89030971374941	41.0	40.0	41.0	38.0	41.0
19	39.889723134678555	41.0	40.0	41.0	38.0	41.0
20	39.86778507742844	41.0	40.0	41.0	38.0	41.0
21	39.82648991083998	41.0	40.0	41.0	38.0	41.0
22	39.791295166588455	41.0	40.0	41.0	38.0	41.0
23	39.76396058188644	41.0	40.0	41.0	38.0	41.0
24	39.74929610511497	41.0	40.0	41.0	38.0	41.0
25	39.726888784608164	41.0	40.0	41.0	38.0	41.0
26	39.60053965274519	41.0	40.0	41.0	37.0	41.0
27	39.4991787893008	41.0	39.0	41.0	37.0	41.0
28	39.493547630220554	41.0	39.0	41.0	37.0	41.0
29	39.442515251055845	41.0	39.0	41.0	37.0	41.0
30	39.41166119192867	41.0	39.0	41.0	36.0	41.0
31	39.341154387611454	41.0	39.0	41.0	36.0	41.0
32	39.3015016424214	41.0	39.0	41.0	36.0	41.0
33	39.195330830595964	41.0	39.0	41.0	35.0	41.0
34	39.19192867198498	41.0	39.0	41.0	35.0	41.0
35	39.06346785546692	41.0	39.0	41.0	35.0	41.0
36	38.986391365556074	41.0	39.0	41.0	35.0	41.0
37	38.96527451900516	40.0	39.0	41.0	35.0	41.0
38	38.85851712810887	40.0	39.0	41.0	35.0	41.0
39	38.72876583763491	40.0	38.0	41.0	35.0	41.0
40	38.68277803847959	40.0	38.0	41.0	35.0	41.0
41	38.611098076020646	40.0	38.0	41.0	35.0	41.0
42	38.58751759737213	40.0	38.0	41.0	35.0	41.0
43	37.81440638198029	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	0.0
22	2.0
23	2.0
24	4.0
25	10.0
26	10.0
27	17.0
28	28.0
29	28.0
30	49.0
31	54.0
32	60.0
33	92.0
34	138.0
35	202.0
36	330.0
37	686.0
38	1655.0
39	5154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5269826372595	16.740966682308773	12.881276396058189	27.850774284373536
2	21.38667292351009	21.105114969497887	31.921633036133272	25.58657907085875
3	20.753167526982637	18.676677616142655	29.950727358047864	30.61942749882684
4	15.391834819333647	16.072266541529796	33.04786485218207	35.48803378695448
5	18.54763022055373	32.83669638667293	31.405443453777572	17.210229938995777
6	33.904270295635854	33.48193336461755	15.614734866259973	16.999061473486627
7	29.927264195213514	27.076489910839978	19.274988268418582	23.721257625527922
8	26.3608634443923	31.100422336931015	20.50680431722196	22.031909901454718
9	26.13796339746598	13.808071328015018	16.787893007977477	43.26607226654153
10	19.87329892069451	22.87658376349132	29.44626935710934	27.803847958704836
11	39.312529328953545	19.767714687939932	18.43031440638198	22.48944157672454
12	22.87658376349132	24.36649460347255	26.243547630220554	26.51337400281558
13	35.01877053026748	17.210229938995777	20.67104645706241	27.09995307367433
14	23.744720788362272	20.57719380572501	22.85312060065697	32.824964805255746
15	30.138432660722664	24.331299859221023	19.192867198498355	26.337400281557954
16	26.020647583294227	24.472078836227123	22.407320506804318	27.09995307367433
17	27.498826841858282	24.483810417644296	21.046457062412014	26.970905678085405
18	26.642421398404505	21.034725480994837	22.759267949319568	29.563585171281087
19	28.49601126231816	22.841389019239795	22.935241670577195	25.72735804786485
20	28.695448146410136	21.492257156264667	22.430783669638668	27.381511027686535
21	27.569216330361336	22.09056780854059	21.99671515720319	28.343500703894886
22	28.671984983575786	23.216799624589395	20.154856874706713	27.95635851712811
23	27.698263725950255	21.48052557484749	21.90286250586579	28.918348193336463
24	28.66025340215861	21.116846550915064	22.25480994838104	27.968090098545282
25	28.648521820741436	22.770999530736745	22.149225715626468	26.431252932895354
26	28.730642890661663	23.18160488033787	21.539183481933364	26.5485687470671
27	27.756921633036136	21.304551853589864	24.1083998122947	26.830126701079305
28	26.70107930549038	22.595025809479118	22.55983106522759	28.144063819802913
29	27.62787423744721	24.284373533552323	20.518535898639136	27.569216330361336
30	26.302205537306428	22.92351008916002	23.791647114030972	26.982637259502578
31	27.13514781792586	22.782731112153918	22.641952135147818	27.44016893477241
32	24.8592210229939	22.149225715626468	23.01736274049742	29.974190520882217
33	26.102768653214454	21.163772876583764	24.167057719380573	28.56640075082121
34	27.04129516658846	21.984983575786014	23.439699671515722	27.53402158610981
35	26.595495072735805	23.287189113092445	24.260910370717973	25.856405443453777
36	26.3608634443923	21.57437822618489	24.31956827780385	27.745190051618955
37	26.13796339746598	21.76208352885969	23.897231346785546	28.202721726888786
38	24.472078836227123	19.66213045518536	26.923979352416705	28.94181135617081
39	24.213984045049273	19.673862036602536	28.07367433129986	28.038479587048332
40	24.143594556546223	20.53026748005631	28.695448146410136	26.630689816987328
41	22.794462693571095	19.767714687939932	29.98592210229939	27.451900516189582
42	21.539183481933364	20.518535898639136	29.93899577663069	28.00328484279681
43	20.354293758798686	20.682778038479587	29.833411543876114	29.12951665884561
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	3.0
12	3.0
13	2.5
14	2.0
15	4.5
16	7.0
17	7.0
18	7.0
19	7.0
20	11.5
21	16.0
22	15.0
23	14.0
24	18.5
25	23.0
26	23.0
27	25.0
28	27.0
29	37.0
30	47.0
31	59.0
32	71.0
33	71.0
34	82.0
35	93.0
36	101.0
37	109.0
38	148.5
39	188.0
40	188.0
41	227.0
42	266.0
43	311.0
44	356.0
45	487.0
46	618.0
47	618.0
48	701.0
49	784.0
50	830.0
51	876.0
52	941.5
53	1007.0
54	1007.0
55	942.0
56	877.0
57	834.0
58	791.0
59	710.5
60	630.0
61	630.0
62	609.0
63	588.0
64	476.5
65	365.0
66	320.5
67	276.0
68	276.0
69	228.0
70	180.0
71	160.0
72	140.0
73	105.0
74	70.0
75	70.0
76	55.5
77	41.0
78	33.5
79	26.0
80	19.0
81	12.0
82	12.0
83	10.0
84	8.0
85	5.0
86	2.0
87	2.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8524.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.49038010323791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.13263372686198	72.54809948381042
2	5.363649613758927	8.634443923040825
3	1.880192391779624	4.540122008446739
4	0.9036583588398193	2.9094321914594086
5	0.4226789097799155	1.7010793054903801
6	0.34980323567993005	1.6893477240732049
7	0.21862702229995626	1.2318160488033787
8	0.23320215711995335	1.5016424213984045
9	0.11660107855997667	0.8446738620366024
>10	0.3789535053199242	4.3993430314406385
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	29	0.340215861098076	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	28	0.328484279680901	RNA PCR Primer, Index 46 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	21	0.24636320976067572	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	20	0.2346316283435007	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	19	0.22290004692632567	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	16	0.18770530267480057	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	16	0.18770530267480057	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	15	0.17597372125762553	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	14	0.1642421398404505	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	14	0.1642421398404505	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	13	0.15251055842327546	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	12	0.14077897700610043	RNA PCR Primer, Index 46 (95% over 22bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	12	0.14077897700610043	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	12	0.14077897700610043	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	12	0.14077897700610043	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	12	0.14077897700610043	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	12	0.14077897700610043	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	12	0.14077897700610043	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	12	0.14077897700610043	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	11	0.1290473955889254	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	11	0.1290473955889254	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	11	0.1290473955889254	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	11	0.1290473955889254	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	10	0.11731581417175035	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	10	0.11731581417175035	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	10	0.11731581417175035	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	9	0.1055842327545753	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	9	0.1055842327545753	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	9	0.1055842327545753	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	9	0.1055842327545753	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	9	0.1055842327545753	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	9	0.1055842327545753	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	9	0.1055842327545753	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	9	0.1055842327545753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.011731581417175035	0.0
27	0.0	0.0	0.0	0.011731581417175035	0.0
28	0.0	0.0	0.0	0.17597372125762553	0.0
29	0.0	0.0	0.0	0.38714218676677614	0.0
30	0.0	0.0	0.0	0.5865790708587517	0.0
31	0.0	0.0	0.0	1.313937118723604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	40	0.0017788276	23.125	37
CTCTTAT	50	0.0064899484	18.499998	36
TCTCTTA	50	0.0064899484	18.499998	35
>>END_MODULE