FastQCFastQC Report
Fri 10 Feb 2017
ERR1632591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343468
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19290.5616243725761934No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14750.4294432086831961No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12000.34937752570836295No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10230.29784434066637944No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10040.29231252984266365No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8340.24281738036731224No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8300.24165278861495104No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8290.24136164067686075No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8230.2396147530483189No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7960.23175375871988074No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7900.23000687109133894No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7830.22796883552470681No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7650.22272817263908137No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7630.22214587676290076No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC7620.22185472882481047No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7480.21777865769154622No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7380.21486717831064323No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7050.20525929635366322No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7030.2046770004774826No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6740.19623371027286382No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT6670.19419567470623172No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6650.19361337883005114No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6580.19157534326341902No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6570.1912841953253287No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6500.18924615975869657No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6450.1877904200682451No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6450.1877904200682451No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6270.18254975718261962No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6160.1793471298636263No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5920.17235957934945903No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5860.17061269172091725No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5850.1703215437828269No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5790.16857465615428513No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5760.16770121234001423No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5670.1650808808972015No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5660.16478973295911117No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5560.16187825357820815No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5470.1592579221353954No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5290.15401725924977No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5230.15227037162122817No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5210.15168807574504758No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5190.15110577986886697No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5090.14819430048796395No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC4980.1449916731689706No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4820.14033330615952577No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4790.13945986234525487No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4660.13567493915008094No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4550.13247231183108762No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG4540.1321811638929973No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4520.13159886801681672No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4500.13101657214063608No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4420.12868738863591367No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4410.12839624069782338No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4390.12781394482164277No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4330.12606705719310096No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4310.12548476131692035No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4280.12461131750264945No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4160.12111754224556583No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4140.12053524636938523No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4140.12053524636938523No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4120.1199529504932046No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4010.11675032317421127No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3980.11587687935994037No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3930.11442113966948886No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3870.11267425204094705No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3870.11267425204094705No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3830.11150966028858583No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3780.11005392059813433No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3620.10539555358868949No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3610.10510440565059918No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3610.10510440565059918No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3600.10481325771250888No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3590.10452210977441859No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA3550.10335751802205737No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3540.10306637008396706No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3530.10277522214587675No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3500.10190177833160587No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3480.10131948245542526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3480.10131948245542526No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA3470.10102833451733494No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC3460.10073718657924463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCT403.8198777E-837.033
TCAATGC502.7113856E-729.632
GCGGACT250.005491045329.632
AGGATAA250.005491045329.634
ACGACGT250.005491045329.65
GGTATCA5950.029.226891
ATACACA851.8189894E-1228.29411737
GAGGGCT604.3042746E-827.75000234
CTTTTTA358.856685E-426.428572
CGTGTTT358.856685E-426.4285726
TTTGCGA358.856685E-426.4285719
GCTTTTT358.856685E-426.428571
ATACCGC509.066875E-625.9000026
ATACGGC809.731593E-1025.437529
TAAGTTC451.3205745E-424.66666630
AAGTTCC451.3205745E-424.66666631
AACGACC451.3205745E-424.66666637
GTAAGTT451.3205745E-424.66666629
GTTATCC851.9645086E-923.94117523
TCCAGTA551.897627E-523.54545416
Produced by FastQC (version 0.11.5)