FastQCFastQC Report
Fri 10 Feb 2017
ERR1632590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225702
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10220.45280945671726436No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8210.3637539764822642No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6330.2804583034266422No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5100.22596166626791075No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4830.21399898981843318No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4660.20646693427616947No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4450.19716263037102022No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4400.1949473199174132No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4340.19228894737308488No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4290.1900736369194779No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4260.1887444506473137No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3940.1745664637442291No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3860.17102196701845798No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3810.168806656564851No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3750.16614828402052262No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3750.16614828402052262No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3720.16481909774835846No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3680.16304684938547287No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3580.15861622847825893No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3550.15728704220609477No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3520.15595785593393058No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3490.1546286696617664No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3300.1462104899380599No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3290.14576742784733854No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3260.14443824157517435No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3160.1400076206679604No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3120.13823537230507485No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3110.13779231021435343No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3050.13513393767002507No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2870.12715882003704No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2830.12538657167415443No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2730.12095595076694048No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2730.12095595076694048No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2710.1200698265854977No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2710.1200698265854977No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2690.11918370240405492No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2690.11918370240405492No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2580.11431001940611957No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT2550.11298083313395538Illumina PCR Primer Index 10 (95% over 22bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2530.1120947089525126No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2460.10899327431746285No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2460.10899327431746285No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2460.10899327431746285No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2440.10810715013602006No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2420.10722102595457728No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2400.1063349017731345No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2380.1054487775916917No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2290.10146121877519915No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2290.10146121877519915No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2270.10057509459375637No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2260.10013203250303498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3250.029.030771
ATACGGC809.677024E-1025.437529
TTAGCTA400.001926408423.1254
TATTAGC400.001926408423.1252
GCGGTAA4800.021.96874823
CGTGCCA4800.021.58333210
CGCGGTA4900.021.52040922
TACGGCT957.068593E-921.42105330
CTCCGTG5000.021.097
AACTCCG5150.020.8349515
GAGGGCT806.9092494E-720.812534
CCGCGGT5000.020.7221
AGCCGCG5100.020.6764719
GGTAATA5100.020.6764725
TCCGTGC5100.020.676478
ATTAGCT450.003816431420.5555553
GTATTAG450.003816431420.5555551
ACCGTAG450.003816431420.5555558
CACCATC450.003816431420.5555557
ATACACA450.003816431420.55555537
Produced by FastQC (version 0.11.5)