##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632590.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225702
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.029175638674005	34.0	33.0	34.0	31.0	34.0
2	33.160153653933065	34.0	33.0	34.0	31.0	34.0
3	33.23349372181018	34.0	33.0	34.0	31.0	34.0
4	36.5219891715625	37.0	37.0	37.0	35.0	37.0
5	36.496960594057654	37.0	37.0	37.0	35.0	37.0
6	36.5409034922154	37.0	37.0	37.0	35.0	37.0
7	36.54853745203853	37.0	37.0	37.0	35.0	37.0
8	36.522348051855985	37.0	37.0	37.0	35.0	37.0
9	38.32033389159157	39.0	39.0	39.0	37.0	39.0
10	38.34179581926611	39.0	39.0	39.0	37.0	39.0
11	38.42244641164013	39.0	39.0	39.0	37.0	39.0
12	38.37398871077793	39.0	39.0	39.0	37.0	39.0
13	38.411843935809166	39.0	39.0	39.0	37.0	39.0
14	39.926828295717364	41.0	40.0	41.0	38.0	41.0
15	39.94236648323896	41.0	40.0	41.0	38.0	41.0
16	39.89536202603433	41.0	40.0	41.0	38.0	41.0
17	39.906451870165085	41.0	40.0	41.0	38.0	41.0
18	39.91554350426669	41.0	40.0	41.0	38.0	41.0
19	39.91658470017988	41.0	40.0	41.0	38.0	41.0
20	39.904874569122114	41.0	40.0	41.0	38.0	41.0
21	39.8565276337826	41.0	40.0	41.0	38.0	41.0
22	39.8276001098794	41.0	40.0	41.0	38.0	41.0
23	39.7855933930581	41.0	40.0	41.0	38.0	41.0
24	39.76620942658904	41.0	40.0	41.0	38.0	41.0
25	39.72211588732045	41.0	40.0	41.0	38.0	41.0
26	39.63076977607642	41.0	40.0	41.0	37.0	41.0
27	39.51852442601306	41.0	39.0	41.0	37.0	41.0
28	39.49964111970652	41.0	39.0	41.0	37.0	41.0
29	39.44132528732577	41.0	39.0	41.0	37.0	41.0
30	39.420036153866604	41.0	39.0	41.0	36.0	41.0
31	39.35031590327068	41.0	39.0	41.0	36.0	41.0
32	39.298770059636155	41.0	39.0	41.0	36.0	41.0
33	39.22083100725736	41.0	39.0	41.0	35.0	41.0
34	39.1943270329904	41.0	39.0	41.0	35.0	41.0
35	39.10884263320662	41.0	39.0	41.0	35.0	41.0
36	39.058896243719595	41.0	39.0	41.0	35.0	41.0
37	39.00039875588165	40.0	39.0	41.0	35.0	41.0
38	38.923908516539505	40.0	39.0	41.0	35.0	41.0
39	38.80627996207389	40.0	38.0	41.0	35.0	41.0
40	38.73690973052964	40.0	38.0	41.0	35.0	41.0
41	38.6773754773994	40.0	38.0	41.0	35.0	41.0
42	38.60310940975268	40.0	38.0	41.0	35.0	41.0
43	37.86024492472375	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	7.0
20	11.0
21	16.0
22	51.0
23	78.0
24	122.0
25	170.0
26	269.0
27	371.0
28	491.0
29	757.0
30	1020.0
31	1259.0
32	1752.0
33	2337.0
34	3585.0
35	5557.0
36	9008.0
37	17306.0
38	45966.0
39	135563.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.15026007744726	16.10840843235771	12.665815987452481	28.075515502742554
2	22.23772939539747	19.574926230161896	31.08257791246865	27.10476646197198
3	21.97632276187185	19.17838565896625	28.801251207344198	30.044040371817704
4	15.76149081532286	16.198350036774155	34.67359615776555	33.36656299013744
5	17.636086521165076	32.39182639055037	32.34308956057102	17.628997527713533
6	32.50436416159361	34.11976854436381	16.0025165926753	17.37335070136729
7	29.342230020115018	27.394086007213055	19.318393279634208	23.94529069303772
8	26.91956650805044	29.497744813958228	20.764104881658117	22.81858379633322
9	28.116277215088925	12.386243808207283	17.09023402539632	42.407244951307476
10	19.98697397453279	22.195195434688216	28.45699196285367	29.360838627925318
11	40.16136321344073	19.174841162240476	18.173077775119406	22.490717849199388
12	22.400333182692222	23.459251579516355	26.34757334892912	27.792841888862306
13	36.7621908534262	17.101310577664353	19.6848056286608	26.451692940248645
14	24.256320280724143	20.084447634491497	22.751238358543567	32.9079937262408
15	30.566853638868952	23.422034363895754	19.232439234034256	26.778672763201033
16	25.14510283471126	23.616538621722448	22.586862322885928	28.651496220680368
17	27.402947249027477	23.12784113565675	21.017979459641474	28.451232155674294
18	27.062232501262727	20.172616990545055	22.845167521776503	29.919982986415718
19	28.94480332473793	21.987842376230603	22.400776244782943	26.666578054248525
20	31.289930970926267	19.79601421343187	21.673268291818417	27.240786523823445
21	28.025449486491038	21.39103774002889	21.370656883855705	29.21285588962437
22	28.816315318428725	22.475210676024137	20.49162169586446	28.21685230968268
23	29.30988648749236	21.01975170800436	21.527500863971078	28.142860940532206
24	28.577504851529895	20.424719320165526	21.935561049525482	29.0622147787791
25	29.19380421972335	21.45528174318349	22.0498710689316	27.30104296816156
26	29.133990837475963	22.147344728890307	21.650229063100905	27.06843537053283
27	27.270471683901782	21.46148461245359	24.259421715359192	27.008621988285437
28	26.975835393572055	22.92757707065068	21.12076986468884	28.97581767108843
29	27.48092617699444	23.796864892646056	21.26742341671762	27.454785513641884
30	27.388326200033674	21.451737246457718	23.76097686329762	27.39895969021099
31	28.739665576733923	21.847391693471923	21.87530460518737	27.537638124606783
32	25.720640490558345	21.523956367245304	22.50578196028391	30.249621181912435
33	26.55182497275168	21.157987080309436	24.04896722226653	28.241220724672356
34	26.707782828685612	21.405658789022695	23.437984599161727	28.448573783129966
35	26.056481555325163	23.123410514749537	23.996685895561402	26.823422034363897
36	26.715757946318597	20.90810006114257	25.02946362903297	27.34667836350586
37	27.486242922083097	20.69188576087053	24.101691611062375	27.720179705983995
38	25.97274282017882	19.972795987629706	25.962995454182945	28.091465738008527
39	25.292642510921482	19.078696688553933	27.003305243196777	28.625355557327804
40	25.018387076764938	20.09818255930386	28.28818530628882	26.59524505764238
41	23.233732975339162	19.44067841667331	28.993097092626563	28.332491515360964
42	21.034815819088887	20.573588182647917	30.526534988613303	27.865061009649892
43	20.185022729085254	21.32413536432996	29.582369673286014	28.908472233298777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	5.0
4	10.0
5	10.0
6	10.5
7	11.0
8	11.5
9	12.0
10	19.0
11	26.0
12	26.0
13	54.0
14	82.0
15	130.5
16	179.0
17	181.5
18	184.0
19	184.0
20	210.5
21	237.0
22	220.5
23	204.0
24	234.5
25	265.0
26	265.0
27	317.0
28	369.0
29	519.0
30	669.0
31	760.5
32	852.0
33	852.0
34	1235.5
35	1619.0
36	1766.5
37	1914.0
38	2657.0
39	3400.0
40	3400.0
41	4501.5
42	5603.0
43	6865.0
44	8127.0
45	12108.5
46	16090.0
47	16090.0
48	18558.0
49	21026.0
50	23723.0
51	26420.0
52	27154.0
53	27888.0
54	27888.0
55	25893.5
56	23899.0
57	22791.0
58	21683.0
59	20645.5
60	19608.0
61	19608.0
62	17824.5
63	16041.0
64	12892.5
65	9744.0
66	8319.5
67	6895.0
68	6895.0
69	5813.0
70	4731.0
71	4026.0
72	3321.0
73	2606.0
74	1891.0
75	1891.0
76	1518.0
77	1145.0
78	895.5
79	646.0
80	515.5
81	385.0
82	385.0
83	319.5
84	254.0
85	188.5
86	123.0
87	98.5
88	74.0
89	74.0
90	63.0
91	52.0
92	32.5
93	13.0
94	9.0
95	5.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	225702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.7277812992442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.88648446036534	38.35942815366033
2	7.238086127237543	6.6196323890282365
3	2.5160006018476135	3.4515337581016348
4	1.41845979916814	2.5945207791252223
5	0.8306601119989085	1.8992121967745887
6	0.5997533298679424	1.6455233461016838
7	0.4698067014529842	1.5038252667872962
8	0.3358618081894023	1.2286572249322958
9	0.2878815496272262	1.1847766087297185
>10	2.005174960112397	17.90877605201166
>50	0.24290005748106883	7.706990122840135
>100	0.16493213779578747	14.532254135309671
>500	0.00299876614174159	0.8977242513052621
>1k	9.995887139138634E-4	0.46714571529224946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1022	0.45280945671726436	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	821	0.3637539764822642	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	633	0.2804583034266422	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	510	0.22596166626791075	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	483	0.21399898981843318	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	466	0.20646693427616947	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	445	0.19716263037102022	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	440	0.1949473199174132	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	434	0.19228894737308488	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	429	0.1900736369194779	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	426	0.1887444506473137	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	394	0.1745664637442291	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	386	0.17102196701845798	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	381	0.168806656564851	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	375	0.16614828402052262	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	375	0.16614828402052262	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	372	0.16481909774835846	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	368	0.16304684938547287	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	358	0.15861622847825893	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	355	0.15728704220609477	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	352	0.15595785593393058	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	349	0.1546286696617664	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	330	0.1462104899380599	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	329	0.14576742784733854	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	326	0.14443824157517435	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	316	0.1400076206679604	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	312	0.13823537230507485	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	311	0.13779231021435343	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	305	0.13513393767002507	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	287	0.12715882003704	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	283	0.12538657167415443	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	273	0.12095595076694048	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	273	0.12095595076694048	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	271	0.1200698265854977	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	271	0.1200698265854977	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	269	0.11918370240405492	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	269	0.11918370240405492	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	258	0.11431001940611957	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	255	0.11298083313395538	Illumina PCR Primer Index 10 (95% over 22bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	253	0.1120947089525126	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	246	0.10899327431746285	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	246	0.10899327431746285	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	246	0.10899327431746285	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	244	0.10810715013602006	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	242	0.10722102595457728	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	240	0.1063349017731345	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	238	0.1054487775916917	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	229	0.10146121877519915	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	229	0.10146121877519915	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	227	0.10057509459375637	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	226	0.10013203250303498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	8.861241814427874E-4	0.0
16	0.0	0.0	0.0	0.0013291862721641812	0.0
17	0.0	0.0	0.0	0.0013291862721641812	0.0
18	0.0	0.0	0.0	0.0017722483628855748	0.0
19	0.0	0.0	0.0	0.0026583725443283623	0.0
20	0.0	0.0	0.0	0.0035444967257711496	0.0
21	0.0	0.0	0.0	0.005316745088656725	0.0
22	0.0	0.0	0.0	0.007975117632985087	0.0
23	0.0	0.0	0.0	0.008861241814427875	0.0
24	0.0	0.0	0.0	0.011076552268034843	0.0
25	0.0	0.0	0.0	0.013734924812363205	0.0
26	0.0	0.0	0.0	0.017279421538134353	0.0
27	0.0	0.0	0.0	0.051838264614403065	0.0
28	0.0	0.0	0.0	0.1896305748287565	0.0
29	0.0	0.0	0.0	0.3854640189276125	0.0
30	0.0	0.0	0.0	0.6787711229851752	0.0
31	0.0	0.0	0.0	1.3681757361476636	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	325	0.0	29.03077	1
ATACGGC	80	9.677024E-10	25.4375	29
TTAGCTA	40	0.0019264084	23.125	4
TATTAGC	40	0.0019264084	23.125	2
GCGGTAA	480	0.0	21.968748	23
CGTGCCA	480	0.0	21.583332	10
CGCGGTA	490	0.0	21.520409	22
TACGGCT	95	7.068593E-9	21.421053	30
CTCCGTG	500	0.0	21.09	7
AACTCCG	515	0.0	20.834951	5
GAGGGCT	80	6.9092494E-7	20.8125	34
CCGCGGT	500	0.0	20.72	21
AGCCGCG	510	0.0	20.67647	19
GGTAATA	510	0.0	20.67647	25
TCCGTGC	510	0.0	20.67647	8
ATTAGCT	45	0.0038164314	20.555555	3
GTATTAG	45	0.0038164314	20.555555	1
ACCGTAG	45	0.0038164314	20.555555	8
CACCATC	45	0.0038164314	20.555555	7
ATACACA	45	0.0038164314	20.555555	37
>>END_MODULE