FastQCFastQC Report
Fri 10 Feb 2017
ERR1632589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT240.8673653776653415RNA PCR Primer, Index 26 (100% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA130.46982291290205996No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA110.39754246476328153No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG110.39754246476328153No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA100.36140224069389226No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA100.36140224069389226No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA100.36140224069389226No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT90.32526201662450305No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA80.28912179255511383No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA80.28912179255511383No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC70.2529815684857246No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG70.2529815684857246No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG70.2529815684857246No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA60.21684134441633537No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC60.21684134441633537RNA PCR Primer, Index 26 (100% over 23bp)
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT60.21684134441633537No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA60.21684134441633537No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC60.21684134441633537No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT60.21684134441633537No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA60.21684134441633537No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC50.18070112034694613No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC50.18070112034694613No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA50.18070112034694613No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG50.18070112034694613No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC50.18070112034694613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.18070112034694613No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC50.18070112034694613No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA50.18070112034694613No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC50.18070112034694613No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA50.18070112034694613No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC50.18070112034694613No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC50.18070112034694613No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC50.18070112034694613No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG50.18070112034694613No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC50.18070112034694613No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC50.18070112034694613No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT50.18070112034694613No Hit
CCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCT40.14456089627755692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40.14456089627755692No Hit
TTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCAT40.14456089627755692No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT40.14456089627755692No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT40.14456089627755692No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACT40.14456089627755692No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCCTGTC40.14456089627755692No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG40.14456089627755692No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG40.14456089627755692No Hit
GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGG40.14456089627755692No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA40.14456089627755692No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA40.14456089627755692No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT40.14456089627755692No Hit
GGGCTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGT40.14456089627755692No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA40.14456089627755692No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAA40.14456089627755692No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT40.14456089627755692No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC40.14456089627755692No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC40.14456089627755692No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT40.14456089627755692No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA40.14456089627755692No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC40.14456089627755692No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGG40.14456089627755692No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.14456089627755692No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC40.14456089627755692No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT40.14456089627755692No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA40.14456089627755692No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC40.14456089627755692No Hit
GGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGA30.10842067220816769No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT30.10842067220816769No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA30.10842067220816769No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT30.10842067220816769No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG30.10842067220816769No Hit
GGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGC30.10842067220816769No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCC30.10842067220816769No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.10842067220816769No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCTGTCTC30.10842067220816769No Hit
CACACATACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG30.10842067220816769No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA30.10842067220816769No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATC30.10842067220816769No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG30.10842067220816769No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA30.10842067220816769No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC30.10842067220816769No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTT30.10842067220816769No Hit
CTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA30.10842067220816769No Hit
GTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACAC30.10842067220816769No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG30.10842067220816769No Hit
CACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT30.10842067220816769No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT30.10842067220816769No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA30.10842067220816769No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC30.10842067220816769No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC30.10842067220816769No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG30.10842067220816769No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG30.10842067220816769No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG30.10842067220816769No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT30.10842067220816769No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG30.10842067220816769No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG30.10842067220816769No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA30.10842067220816769No Hit
TCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGT30.10842067220816769No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT30.10842067220816769No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC30.10842067220816769No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC30.10842067220816769No Hit
CATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCA30.10842067220816769No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC30.10842067220816769No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCG30.10842067220816769No Hit
ACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCG30.10842067220816769No Hit
AACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA30.10842067220816769No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT30.10842067220816769No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG30.10842067220816769No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT30.10842067220816769No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCC30.10842067220816769No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCC30.10842067220816769No Hit
AGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTG30.10842067220816769No Hit
CGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGG30.10842067220816769No Hit
ACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTG30.10842067220816769No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG30.10842067220816769No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC30.10842067220816769No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG30.10842067220816769No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTG30.10842067220816769No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers