FastQCFastQC Report
Fri 10 Feb 2017
ERR1632588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297355
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12240.41162919742395454No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8860.2979603504228952No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6960.23406366128028788No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6290.21153167089842106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5640.18967227724437122No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5600.18832708378873736No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5440.182946309966202No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5390.18126481814665973No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5150.1731936574128567No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5060.17016697213768056No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5010.16848548031813826No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4950.16646769013468748No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4890.16444989995123674No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4800.1614232146760606No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4690.15772393267306756No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4570.15368835230616604No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4540.15267945721444065No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4280.14393569975282072No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4260.14326310302500378No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4140.13922752265810226No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4080.1372097324746515No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4070.13687343411074304No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3840.12913857174084847No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3840.12913857174084847No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3830.12880227337694003No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3650.12274890282658775No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3420.11501404045669318No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3410.11467774209278471No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3380.11366884700105935No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3360.11299625027324242No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3340.1123236535454255No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3270.10996956499806627No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3160.10627028299507323No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3150.10593398463116478No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT3120.10492508953943938TruSeq Adapter, Index 12 (95% over 22bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3090.10391619444771402No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3080.10357989608380555No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2980.10021691244472095No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2980.10021691244472095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC850.030.47058929
AAACTCT250.005489874629.5999985
TAGGTAG250.005489874629.5999986
GGTATCA4650.028.247311
GGACGTA601.331844E-624.66666627
CAGTCGG701.9129584E-723.78571510
CTTAGGA551.8965156E-523.5454542
CCTCTAG400.001927840823.1251
AAACACG652.6717553E-622.7692315
ACGAAAG652.6717553E-622.7692319
CTCTATG903.799869E-922.6111131
GCAGTCG753.7215068E-722.29
CTGTGCA753.7215068E-722.29
AGTCGGT753.7215068E-722.211
TAGGACC502.6955802E-422.1999994
CCGTTTA502.6955802E-422.19999927
GCGGTAA4900.021.52040723
TACGGCT1301.8189894E-1221.34615330
ACACGAA705.080963E-621.14285917
TAAAGAC705.080963E-621.14285937
Produced by FastQC (version 0.11.5)