##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297355
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0653326831565	34.0	33.0	34.0	31.0	34.0
2	33.20040355803669	34.0	33.0	34.0	31.0	34.0
3	33.270969043735604	34.0	34.0	34.0	31.0	34.0
4	36.546488204334885	37.0	37.0	37.0	35.0	37.0
5	36.52369726421281	37.0	37.0	37.0	35.0	37.0
6	36.57186191589178	37.0	37.0	37.0	35.0	37.0
7	36.57394023978073	37.0	37.0	37.0	35.0	37.0
8	36.55258529367254	37.0	37.0	37.0	35.0	37.0
9	38.34474617880984	39.0	39.0	39.0	37.0	39.0
10	38.37079921306183	39.0	39.0	39.0	37.0	39.0
11	38.44523549292933	39.0	39.0	39.0	37.0	39.0
12	38.40253568966387	39.0	39.0	39.0	37.0	39.0
13	38.44070891695112	39.0	39.0	39.0	37.0	39.0
14	39.97613963108069	41.0	40.0	41.0	38.0	41.0
15	39.98490692942779	41.0	40.0	41.0	38.0	41.0
16	39.939321686199996	41.0	40.0	41.0	38.0	41.0
17	39.94621916564376	41.0	40.0	41.0	38.0	41.0
18	39.95747170890014	41.0	40.0	41.0	38.0	41.0
19	39.95272653898539	41.0	40.0	41.0	38.0	41.0
20	39.9388575944578	41.0	40.0	41.0	38.0	41.0
21	39.889256948764945	41.0	40.0	41.0	38.0	41.0
22	39.86572951522591	41.0	40.0	41.0	38.0	41.0
23	39.82900573388711	41.0	40.0	41.0	38.0	41.0
24	39.816663583931664	41.0	40.0	41.0	38.0	41.0
25	39.774918868019704	41.0	40.0	41.0	38.0	41.0
26	39.68673807401927	41.0	40.0	41.0	38.0	41.0
27	39.585905735568595	41.0	40.0	41.0	37.0	41.0
28	39.560071295253145	41.0	40.0	41.0	37.0	41.0
29	39.5185855290814	41.0	40.0	41.0	37.0	41.0
30	39.48977484824536	41.0	40.0	41.0	37.0	41.0
31	39.419185821660975	41.0	39.0	41.0	36.0	41.0
32	39.379075515797616	41.0	39.0	41.0	36.0	41.0
33	39.30281986178137	41.0	39.0	41.0	36.0	41.0
34	39.28042911671235	41.0	39.0	41.0	36.0	41.0
35	39.20414319584335	41.0	39.0	41.0	35.0	41.0
36	39.16131559919961	41.0	39.0	41.0	35.0	41.0
37	39.099826806342584	41.0	39.0	41.0	35.0	41.0
38	39.03249314792084	41.0	39.0	41.0	35.0	41.0
39	38.917226883691214	41.0	39.0	41.0	35.0	41.0
40	38.84799986548065	40.0	38.0	41.0	35.0	41.0
41	38.79255099123943	40.0	38.0	41.0	35.0	41.0
42	38.71481226143835	40.0	38.0	41.0	35.0	41.0
43	38.00158060231037	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	5.0
19	11.0
20	16.0
21	26.0
22	51.0
23	105.0
24	140.0
25	239.0
26	328.0
27	457.0
28	649.0
29	868.0
30	1182.0
31	1587.0
32	2150.0
33	2908.0
34	4403.0
35	7020.0
36	11014.0
37	21514.0
38	57947.0
39	184733.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.11614063997579	16.220342688032822	12.742345008491535	27.921171663499855
2	22.13986648954953	19.522456323249987	31.36587580501421	26.97180138218628
3	21.67442955390022	19.379193220224984	28.92704006995006	30.019337155924735
4	15.787190395318726	16.038068974794438	34.6370499907518	33.53769063913504
5	17.627078744261908	32.54863715088026	32.468261841906134	17.35602226295169
6	33.34734576516285	33.940576079097376	15.634510938104288	17.077567217635487
7	29.790990566830892	27.125153436128535	19.553059474365657	23.53079652267492
8	27.185350843268147	29.9860436178978	20.536059592070085	22.29254594676397
9	27.88047956146693	12.446402448252089	17.378554253333558	42.294563736947424
10	19.832187116409678	22.646668123959575	28.823460173866255	28.697684585764488
11	40.033966134754756	19.1491651393116	18.463452775302247	22.3534159506314
12	22.73477829530359	23.498175581375797	26.71285164197676	27.054194481343846
13	36.58018193741487	17.427653814464193	19.802592860385733	26.1895713877352
14	24.190613912663313	19.933412923946126	22.868288745775253	33.00768441761531
15	30.276941702678617	23.67675001261119	19.361033108573928	26.68527517613627
16	25.383127911082713	23.79210035143179	22.473138168182814	28.351633569302688
17	27.395873619074845	23.2240924147904	21.433976223705674	27.94605774242908
18	27.02359133022818	20.503438650770963	22.902254880530005	29.57071513847085
19	28.653293201728573	22.332901750432985	22.825915151922786	26.187889895915657
20	30.683526424643944	20.341679137730996	21.935397084293186	27.03939735333188
21	27.890232214020276	21.42994064333877	21.653242756973988	29.026584385666965
22	28.612937398059557	22.524928116224714	21.086243715424324	27.7758907702914
23	28.956634325974008	21.42086058751324	21.594726841653916	28.027778244858837
24	28.341544618385434	20.789964856820973	22.387718383750062	28.48077214104353
25	28.860452993896185	21.7729649745254	22.236384119991257	27.13019791158716
26	28.681205965932975	22.485244909283516	21.649879773334906	27.183669351448604
27	27.192413108910223	21.653915353701805	24.23971347379395	26.913958063594016
28	26.526206050007566	23.075112239578953	21.348220140909014	29.050461569504467
29	27.488691967513578	23.646146861495517	21.63575524205075	27.229405928940153
30	27.07168199626709	21.74000773486237	23.77898471523936	27.40932555363118
31	28.58367944039952	22.121370079534564	22.164752568478754	27.13019791158716
32	25.722789258630257	21.621294412402683	22.79161271880412	29.864303610162935
33	26.386305930621646	21.710413478838426	23.92561080190345	27.97766978863648
34	26.518807486001585	21.518050814682788	23.609154041465587	28.353987657850045
35	26.08666408837921	23.270837887373677	23.987825999226516	26.6546720250206
36	26.610953237712497	21.562105900354794	24.625447697196954	27.20149316473575
37	27.092532494829413	21.037816751021506	24.306973146575643	27.562677607573438
38	26.010660658135897	20.36925560357149	25.592641791797682	28.02744194649493
39	25.201863092936055	19.53960753980932	26.84602579408451	28.412503573170117
40	25.027660540431473	20.53101511661146	27.74293353062837	26.698390812328697
41	23.392241596744633	19.71851826940862	28.605202535689667	28.284037598157084
42	21.46054379445444	20.533705503522725	30.28064098468161	27.725109717341223
43	20.782229994451075	21.393620420036658	29.213902574363974	28.610247011148292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.0
2	1.0
3	7.0
4	13.0
5	13.0
6	13.5
7	14.0
8	17.0
9	20.0
10	24.5
11	29.0
12	29.0
13	63.5
14	98.0
15	167.0
16	236.0
17	245.0
18	254.0
19	254.0
20	311.0
21	368.0
22	340.0
23	312.0
24	382.0
25	452.0
26	452.0
27	577.5
28	703.0
29	895.0
30	1087.0
31	1326.5
32	1566.0
33	1566.0
34	2086.0
35	2606.0
36	2900.0
37	3194.0
38	4218.5
39	5243.0
40	5243.0
41	6660.0
42	8077.0
43	9691.0
44	11305.0
45	16102.5
46	20900.0
47	20900.0
48	24019.0
49	27138.0
50	30273.0
51	33408.0
52	34512.0
53	35616.0
54	35616.0
55	33542.5
56	31469.0
57	30367.5
58	29266.0
59	27491.5
60	25717.0
61	25717.0
62	23069.5
63	20422.0
64	16630.0
65	12838.0
66	11010.0
67	9182.0
68	9182.0
69	7695.5
70	6209.0
71	5161.0
72	4113.0
73	3172.5
74	2232.0
75	2232.0
76	1769.0
77	1306.0
78	1062.0
79	818.0
80	652.5
81	487.0
82	487.0
83	411.5
84	336.0
85	245.5
86	155.0
87	125.5
88	96.0
89	96.0
90	67.0
91	38.0
92	28.0
93	18.0
94	12.5
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	297355.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.085294703124795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.53192142132548	45.591259231604994
2	6.040371982082758	6.292291096065569
3	1.8813433475514403	2.939709680849403
4	0.9969497676312633	2.077056898051445
5	0.6421574177990048	1.6723479175929399
6	0.39737277579128244	1.2418366880452605
7	0.30658563722684895	1.1178022286694016
8	0.24944978587167455	1.0394132488606034
9	0.17248806416377951	0.8085682489267758
>10	1.4616488905749843	15.079976999335686
>50	0.1776171722119378	6.437855733777336
>100	0.13498905088107271	13.291849187295623
>500	0.006458806262252283	1.9982684359428005
>1k	6.458806262252284E-4	0.4117644049821529
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1224	0.41162919742395454	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	886	0.2979603504228952	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	696	0.23406366128028788	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	629	0.21153167089842106	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	564	0.18967227724437122	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	560	0.18832708378873736	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	544	0.182946309966202	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	539	0.18126481814665973	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	515	0.1731936574128567	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	506	0.17016697213768056	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	501	0.16848548031813826	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	495	0.16646769013468748	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	489	0.16444989995123674	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	480	0.1614232146760606	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	469	0.15772393267306756	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	457	0.15368835230616604	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	454	0.15267945721444065	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	428	0.14393569975282072	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	426	0.14326310302500378	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	414	0.13922752265810226	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	408	0.1372097324746515	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	407	0.13687343411074304	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	384	0.12913857174084847	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	384	0.12913857174084847	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	383	0.12880227337694003	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	365	0.12274890282658775	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	342	0.11501404045669318	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	341	0.11467774209278471	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	338	0.11366884700105935	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	336	0.11299625027324242	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	334	0.1123236535454255	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	327	0.10996956499806627	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	316	0.10627028299507323	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	315	0.10593398463116478	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	312	0.10492508953943938	TruSeq Adapter, Index 12 (95% over 22bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	309	0.10391619444771402	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	308	0.10357989608380555	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	298	0.10021691244472095	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	298	0.10021691244472095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.725967278169192E-4	0.0
4	0.0	0.0	0.0	6.725967278169192E-4	0.0
5	0.0	0.0	0.0	6.725967278169192E-4	0.0
6	0.0	0.0	0.0	6.725967278169192E-4	0.0
7	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
8	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
9	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
10	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
11	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
12	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
13	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
14	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
15	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
16	0.0	0.0	0.0	6.725967278169192E-4	3.362983639084596E-4
17	0.0	0.0	0.0	0.0013451934556338383	3.362983639084596E-4
18	0.0	0.0	0.0	0.0023540885473592173	6.725967278169192E-4
19	0.0	0.0	0.0	0.003026685275176136	6.725967278169192E-4
20	0.0	0.0	0.0	0.003026685275176136	6.725967278169192E-4
21	0.0	0.0	0.0	0.005044475458626894	6.725967278169192E-4
22	0.0	0.0	0.0	0.006725967278169192	6.725967278169192E-4
23	0.0	0.0	0.0	0.00908005582552841	6.725967278169192E-4
24	0.0	0.0	0.0	0.011097846008979166	6.725967278169192E-4
25	0.0	0.0	0.0	0.012106741100704544	6.725967278169192E-4
26	0.0	0.0	0.0	0.01546972473978914	6.725967278169192E-4
27	0.0	0.0	0.0	0.04304619058028283	6.725967278169192E-4
28	0.0	0.0	0.0	0.15200686048662373	6.725967278169192E-4
29	0.0	0.0	0.0	0.30804930134014896	6.725967278169192E-4
30	0.0	0.0	0.0	0.545139647895613	6.725967278169192E-4
31	0.0	0.0	0.0	1.097677859797212	6.725967278169192E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGC	85	0.0	30.470589	29
AAACTCT	25	0.0054898746	29.599998	5
TAGGTAG	25	0.0054898746	29.599998	6
GGTATCA	465	0.0	28.24731	1
GGACGTA	60	1.331844E-6	24.666666	27
CAGTCGG	70	1.9129584E-7	23.785715	10
CTTAGGA	55	1.8965156E-5	23.545454	2
CCTCTAG	40	0.0019278408	23.125	1
AAACACG	65	2.6717553E-6	22.76923	15
ACGAAAG	65	2.6717553E-6	22.76923	19
CTCTATG	90	3.799869E-9	22.611113	1
GCAGTCG	75	3.7215068E-7	22.2	9
CTGTGCA	75	3.7215068E-7	22.2	9
AGTCGGT	75	3.7215068E-7	22.2	11
TAGGACC	50	2.6955802E-4	22.199999	4
CCGTTTA	50	2.6955802E-4	22.199999	27
GCGGTAA	490	0.0	21.520407	23
TACGGCT	130	1.8189894E-12	21.346153	30
ACACGAA	70	5.080963E-6	21.142859	17
TAAAGAC	70	5.080963E-6	21.142859	37
>>END_MODULE