FastQCFastQC Report
Fri 10 Feb 2017
ERR1632587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111377
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2940.2639683238011439No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2470.22176930605062087No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1750.15712400226258563No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1580.1418605277570773No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1560.14006482487407634No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1480.13288201334207242No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1330.11941424171956509No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1310.1176185388365641No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1310.1176185388365641No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1220.10953787586305971No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1190.10684432153855823No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1180.10594647009705774No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1180.10594647009705774No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1130.1014572128895553RNA PCR Primer, Index 17 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1120.1005593614480548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1550.029.838711
CATTAAA250.005475314329.61
CTGTTAT250.005475314329.69
AGCACTC250.005475314329.65
AATGAGC659.233554E-825.61538534
AACGTCA901.382432E-1024.66666828
CGGGTAA851.9281288E-923.94117723
GGTAACG851.9281288E-923.94117725
GCGGGTA851.9281288E-923.94117722
CTGCGGG952.728484E-1023.36842220
TTCAAGA400.00192031423.1252
CCCACTA400.00192031423.12534
GTAACGT903.7343852E-922.61111326
TGCGGGT1005.18412E-1022.221
TCAATGA753.6783968E-722.232
GCGGTAA855.075526E-821.76470623
GTCAATG855.075526E-821.76470631
ACGTCAA855.075526E-821.76470629
CGGTAAT855.075526E-821.76470624
CGTCAAT855.075526E-821.76470630
Produced by FastQC (version 0.11.5)