FastQCFastQC Report
Fri 10 Feb 2017
ERR1632586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17660
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA640.362400906002265No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT610.3454133635334088TruSeq Adapter, Index 11 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA480.2718006795016987No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA460.26047565118912797No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT370.20951302378255945No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA360.20385050962627407No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT340.19252548131370328No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC310.1755379388448471No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT310.1755379388448471No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA300.16987542468856173No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC290.16421291053227632No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC280.15855039637599094No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC280.15855039637599094No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC280.15855039637599094TruSeq Adapter, Index 11 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC280.15855039637599094No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG270.15288788221970553No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA260.14722536806342015No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC240.13590033975084936No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA240.13590033975084936No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA240.13590033975084936No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT230.13023782559456398No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG230.13023782559456398No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA230.13023782559456398No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA220.1245753114382786No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA210.11891279728199322No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC200.11325028312570783RNA PCR Primer, Index 11 (95% over 24bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC200.11325028312570783No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC190.10758776896942243No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC190.10758776896942243No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT180.10192525481313704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA250.005352779329.61
GCCGCGG250.005352779329.620
TATCAAC500.00677068118.51
CTGTCTC1654.5179317E-511.21212137
Produced by FastQC (version 0.11.5)