##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632586.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17660
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.043771234428085	34.0	33.0	34.0	31.0	34.0
2	33.171177802944506	34.0	33.0	34.0	31.0	34.0
3	33.22893544733862	34.0	34.0	34.0	31.0	34.0
4	36.54286523216308	37.0	37.0	37.0	35.0	37.0
5	36.503907134767836	37.0	37.0	37.0	35.0	37.0
6	36.54331823329559	37.0	37.0	37.0	35.0	37.0
7	36.539014722536805	37.0	37.0	37.0	35.0	37.0
8	36.51438278595697	37.0	37.0	37.0	35.0	37.0
9	38.33929784824462	39.0	39.0	39.0	37.0	39.0
10	38.35356738391846	39.0	39.0	39.0	37.0	39.0
11	38.424518686296715	39.0	39.0	39.0	37.0	39.0
12	38.38335220838052	39.0	39.0	39.0	37.0	39.0
13	38.4218006795017	39.0	39.0	39.0	37.0	39.0
14	39.95317100792752	41.0	40.0	41.0	38.0	41.0
15	39.956058890147226	41.0	40.0	41.0	38.0	41.0
16	39.935447338618346	41.0	40.0	41.0	38.0	41.0
17	39.90311438278596	41.0	40.0	41.0	38.0	41.0
18	39.91562853907135	41.0	40.0	41.0	38.0	41.0
19	39.92961494903737	41.0	40.0	41.0	38.0	41.0
20	39.9218006795017	41.0	40.0	41.0	38.0	41.0
21	39.87797281993205	41.0	40.0	41.0	38.0	41.0
22	39.854699886749714	41.0	40.0	41.0	38.0	41.0
23	39.81806342015855	41.0	40.0	41.0	38.0	41.0
24	39.79915062287656	41.0	40.0	41.0	38.0	41.0
25	39.77202718006795	41.0	40.0	41.0	38.0	41.0
26	39.668120045300114	41.0	40.0	41.0	37.0	41.0
27	39.56404303510759	41.0	40.0	41.0	37.0	41.0
28	39.49813137032842	41.0	40.0	41.0	37.0	41.0
29	39.467100792751985	41.0	39.0	41.0	37.0	41.0
30	39.411778029445074	41.0	39.0	41.0	37.0	41.0
31	39.393714609286526	41.0	39.0	41.0	36.0	41.0
32	39.344960362400904	41.0	39.0	41.0	36.0	41.0
33	39.268516421291054	41.0	39.0	41.0	36.0	41.0
34	39.263590033975085	41.0	39.0	41.0	35.0	41.0
35	39.17372593431484	41.0	39.0	41.0	35.0	41.0
36	39.0826160815402	41.0	39.0	41.0	35.0	41.0
37	38.999150622876556	41.0	39.0	41.0	35.0	41.0
38	38.92208380520951	40.0	39.0	41.0	35.0	41.0
39	38.82797281993205	40.0	38.0	41.0	35.0	41.0
40	38.766817667044165	40.0	38.0	41.0	35.0	41.0
41	38.73380520951302	40.0	38.0	41.0	35.0	41.0
42	38.62797281993205	40.0	38.0	41.0	35.0	41.0
43	37.871517553793886	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	6.0
23	5.0
24	5.0
25	8.0
26	21.0
27	34.0
28	43.0
29	66.0
30	82.0
31	91.0
32	133.0
33	175.0
34	277.0
35	424.0
36	710.0
37	1353.0
38	3466.0
39	10756.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.327293318233295	16.653454133635336	12.97281993204983	28.04643261608154
2	21.761041902604756	19.56964892412231	31.31370328425821	27.355605889014722
3	20.996602491506227	20.130237825594563	28.742921857304644	30.130237825594563
4	15.792751981879954	15.7361268403171	33.92978482446206	34.54133635334088
5	17.502831257078142	32.9841449603624	32.57644394110985	16.936579841449603
6	34.229898074745186	34.331823329558325	15.011325028312573	16.42695356738392
7	29.354473386183464	27.802944507361264	19.64326160815402	23.199320498301248
8	27.60475651189128	30.033975084937715	20.7361268403171	21.62514156285391
9	28.10872027180068	12.553793884484712	16.851642129105322	42.48584371460928
10	19.371460928652322	22.514156285390712	29.24688561721404	28.867497168742922
11	40.34541336353341	18.924122310305776	18.250283125707814	22.480181200453
12	22.938844847112115	23.556058890147224	25.94563986409966	27.559456398640997
13	35.6964892412231	17.06681766704417	20.560588901472254	26.676104190260475
14	24.518686296715742	20.05096262740657	21.857304643261607	33.57304643261608
15	29.4960362400906	24.122310305775766	19.127972819932047	27.253680634201586
16	26.14382785956965	23.850509626274068	21.60815402038505	28.397508493771234
17	27.0158550396376	23.827859569648922	21.325028312570783	27.831257078142695
18	26.69875424688562	20.80973952434881	23.0237825594564	29.467723669309176
19	28.827859569648922	21.710079275198186	23.06908267270668	26.392978482446207
20	30.583238958097397	20.45866364665912	21.800679501698756	27.157417893544732
21	27.7123442808607	21.851642129105322	21.245753114382786	29.190260475651193
22	29.450736126840315	22.180067950169878	20.396375990939976	27.97281993204983
23	28.72593431483579	21.494903737259342	21.58550396375991	28.19365798414496
24	28.289920724801814	21.024915062287654	22.82559456398641	27.859569648924122
25	28.4881087202718	21.998867497168742	22.02718006795017	27.485843714609288
26	28.595696489241224	22.08946772366931	21.387315968289922	27.927519818799546
27	26.359003397508495	22.463193657984146	23.822197055492637	27.355605889014722
28	26.14382785956965	22.355605889014722	22.08946772366931	29.411098527746322
29	27.112117780294447	23.53907134767837	21.834654586636468	27.514156285390712
30	27.072480181200454	21.268403171007925	23.64099660249151	28.018120045300115
31	27.96149490373726	22.684031710079275	21.90260475651189	27.451868629671573
32	26.15515288788222	21.56285390713477	22.446206115515288	29.835787089467726
33	26.121177802944505	21.72140430351076	24.439411098527746	27.718006795016986
34	26.217440543601363	22.129105322763305	23.590033975084935	28.063420158550397
35	26.01359003397509	23.09739524348811	24.813137032842583	26.075877689694227
36	26.625141562853905	21.613816534541336	24.39977349943375	27.361268403171007
37	27.0554926387316	21.551528878822197	23.94110985277463	27.451868629671573
38	25.962627406568515	20.877689694224237	25.30577576443941	27.853907134767837
39	25.15855039637599	19.926387315968288	26.862967157417895	28.052095130237824
40	24.949037372593434	19.988674971687427	28.165345413363536	26.896942242355603
41	23.53907134767837	20.385050962627407	28.08040770101925	27.99546998867497
42	22.07248018120045	20.288788221970556	30.30011325028313	27.338618346545868
43	20.74745186862967	21.013590033975085	29.75651189127973	28.482446206115515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	1.5
9	0.0
10	1.0
11	2.0
12	2.0
13	2.0
14	2.0
15	14.5
16	27.0
17	24.0
18	21.0
19	21.0
20	22.0
21	23.0
22	22.5
23	22.0
24	25.0
25	28.0
26	28.0
27	31.0
28	34.0
29	62.5
30	91.0
31	97.5
32	104.0
33	104.0
34	136.0
35	168.0
36	202.5
37	237.0
38	326.0
39	415.0
40	415.0
41	469.0
42	523.0
43	611.0
44	699.0
45	937.0
46	1175.0
47	1175.0
48	1354.0
49	1533.0
50	1678.0
51	1823.0
52	1972.0
53	2121.0
54	2121.0
55	1947.5
56	1774.0
57	1742.0
58	1710.0
59	1604.0
60	1498.0
61	1498.0
62	1355.0
63	1212.0
64	998.5
65	785.0
66	669.0
67	553.0
68	553.0
69	473.0
70	393.0
71	326.0
72	259.0
73	207.5
74	156.0
75	156.0
76	130.5
77	105.0
78	84.0
79	63.0
80	49.5
81	36.0
82	36.0
83	33.0
84	30.0
85	24.0
86	18.0
87	14.5
88	11.0
89	11.0
90	7.5
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	17660.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.69988674971687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.32625833838689	65.97961494903737
2	6.208308065494239	9.275198187995471
3	2.1983020012128565	4.92638731596829
4	1.0006064281382656	2.9898074745186864
5	0.5003032140691328	1.868629671574179
6	0.4851425106124924	2.17440543601359
7	0.2425712553062462	1.2684031710079275
8	0.15918738629472406	0.9513023782559458
9	0.17434808975136448	1.1721404303510758
>10	0.6898120072771377	8.68629671574179
>50	0.015160703456640388	0.7078142695356738
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	64	0.362400906002265	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	61	0.3454133635334088	TruSeq Adapter, Index 11 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	48	0.2718006795016987	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	46	0.26047565118912797	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	37	0.20951302378255945	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	36	0.20385050962627407	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	34	0.19252548131370328	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	31	0.1755379388448471	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	31	0.1755379388448471	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	30	0.16987542468856173	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	29	0.16421291053227632	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	28	0.15855039637599094	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	28	0.15855039637599094	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	28	0.15855039637599094	TruSeq Adapter, Index 11 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	28	0.15855039637599094	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	27	0.15288788221970553	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	26	0.14722536806342015	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	24	0.13590033975084936	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	24	0.13590033975084936	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	24	0.13590033975084936	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	23	0.13023782559456398	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	23	0.13023782559456398	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	23	0.13023782559456398	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	22	0.1245753114382786	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	21	0.11891279728199322	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	20	0.11325028312570783	RNA PCR Primer, Index 11 (95% over 24bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	20	0.11325028312570783	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	19	0.10758776896942243	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	19	0.10758776896942243	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	18	0.10192525481313704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0056625141562853904	0.0
21	0.0	0.0	0.0	0.0056625141562853904	0.0
22	0.0	0.0	0.0	0.0056625141562853904	0.0
23	0.0	0.0	0.0	0.0056625141562853904	0.0
24	0.0	0.0	0.0	0.0056625141562853904	0.0
25	0.0	0.0	0.0	0.0056625141562853904	0.0
26	0.0	0.0	0.0	0.011325028312570781	0.0
27	0.0	0.0	0.0	0.03397508493771234	0.0
28	0.0	0.0	0.0	0.10758776896942242	0.0
29	0.0	0.0	0.0	0.2887882219705549	0.0
30	0.0	0.0	0.0	0.4813137032842582	0.0
31	0.0	0.0	0.0	0.9116647791619479	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	25	0.0053527793	29.6	1
GCCGCGG	25	0.0053527793	29.6	20
TATCAAC	50	0.006770681	18.5	1
CTGTCTC	165	4.5179317E-5	11.212121	37
>>END_MODULE