ERR1632585.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632585.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19697
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	87	0.4416916281667259	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	78	0.39599939077016805	TruSeq Adapter, Index 11 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	58	0.29446108544448396	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	48	0.24369193278164186	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	47	0.23861501751535766	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	44	0.22338427171650507	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	41	0.20815352591765243	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	40	0.20307661065136823	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	40	0.20307661065136823	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	39	0.19799969538508402	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	38	0.19292278011879982	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	38	0.19292278011879982	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	36	0.1827689495862314	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	36	0.1827689495862314	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	35	0.1776920343199472	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	35	0.1776920343199472	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	34	0.17261511905366297	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	34	0.17261511905366297	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	34	0.17261511905366297	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	33	0.1675382037873788	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	31	0.15738437325481036	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	30	0.15230745798852616	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	30	0.15230745798852616	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	30	0.15230745798852616	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	29	0.14723054272224198	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	28	0.14215362745595778	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	27	0.13707671218967354	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	27	0.13707671218967354	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	26	0.13199979692338934	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	26	0.13199979692338934	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	26	0.13199979692338934	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	26	0.13199979692338934	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	25	0.12692288165710516	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	24	0.12184596639082093	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	24	0.12184596639082093	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	24	0.12184596639082093	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	24	0.12184596639082093	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	23	0.11676905112453673	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	22	0.11169213585825254	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	22	0.11169213585825254	RNA PCR Primer, Index 11 (95% over 22bp)
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	21	0.10661522059196832	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	20	0.10153830532568411	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	20	0.10153830532568411	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	7.903722E-6	37.0	1
TCACTAA	25	0.005367502	29.6	33
GTATCAA	45	1.259735E-4	24.666666	2
CCGTGCC	55	1.7823802E-5	23.545454	9
AACTCCG	55	1.7823802E-5	23.545454	5
GCAGCCG	55	1.7823802E-5	23.545454	17
TAACTCC	55	1.7823802E-5	23.545454	4
CTAACTC	55	1.7823802E-5	23.545454	3
ATACGGA	55	1.7823802E-5	23.545454	29
GCTAACT	55	1.7823802E-5	23.545454	2
CGTGCCA	55	1.7823802E-5	23.545454	10
GGCTAAC	55	1.7823802E-5	23.545454	1
TCCGTGC	55	1.7823802E-5	23.545454	8
AATACGG	55	1.7823802E-5	23.545454	28
CTCCGTG	55	1.7823802E-5	23.545454	7
TACGGAG	60	3.4923352E-5	21.583334	30
GCGGTAA	60	3.4923352E-5	21.583334	23
CGCGGTA	60	3.4923352E-5	21.583334	22
AGCCGCG	60	3.4923352E-5	21.583334	19
TCTTATA	60	3.4923352E-5	21.583334	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content