##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632585.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19697
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.923135502868455	34.0	33.0	34.0	31.0	34.0
2	33.09011524597654	34.0	33.0	34.0	31.0	34.0
3	33.1522059196832	34.0	33.0	34.0	31.0	34.0
4	36.47829618723664	37.0	37.0	37.0	35.0	37.0
5	36.4340762552673	37.0	37.0	37.0	35.0	37.0
6	36.46880235568868	37.0	37.0	37.0	35.0	37.0
7	36.49103924455501	37.0	37.0	37.0	35.0	37.0
8	36.45712545057623	37.0	37.0	37.0	35.0	37.0
9	38.230542722241964	39.0	39.0	39.0	37.0	39.0
10	38.271767274204194	39.0	39.0	39.0	37.0	39.0
11	38.33959486216175	39.0	39.0	39.0	37.0	39.0
12	38.30116261359598	39.0	39.0	39.0	37.0	39.0
13	38.32268873432503	39.0	39.0	39.0	37.0	39.0
14	39.81611412905519	41.0	40.0	41.0	38.0	41.0
15	39.80819414123978	41.0	40.0	41.0	38.0	41.0
16	39.762451134690565	41.0	40.0	41.0	38.0	41.0
17	39.78961263136518	41.0	40.0	41.0	38.0	41.0
18	39.79529877646342	41.0	40.0	41.0	38.0	41.0
19	39.8021526120729	41.0	40.0	41.0	38.0	41.0
20	39.76722343504087	41.0	40.0	41.0	38.0	41.0
21	39.72005889221709	41.0	40.0	41.0	38.0	41.0
22	39.6791897243235	41.0	40.0	41.0	38.0	41.0
23	39.675534345331776	41.0	40.0	41.0	38.0	41.0
24	39.62121135198254	41.0	40.0	41.0	37.0	41.0
25	39.58242371934812	41.0	40.0	41.0	37.0	41.0
26	39.491902320150274	41.0	39.0	41.0	37.0	41.0
27	39.36579174493578	41.0	39.0	41.0	37.0	41.0
28	39.3455348530233	41.0	39.0	41.0	36.0	41.0
29	39.307813372594815	41.0	39.0	41.0	36.0	41.0
30	39.27455957760065	41.0	39.0	41.0	36.0	41.0
31	39.21668274356501	40.0	39.0	41.0	36.0	41.0
32	39.181652028227646	40.0	39.0	41.0	36.0	41.0
33	39.114941361628674	40.0	39.0	41.0	35.0	41.0
34	39.070975275422654	40.0	39.0	41.0	35.0	41.0
35	39.00588922170889	40.0	39.0	41.0	35.0	41.0
36	38.95877544803777	40.0	39.0	41.0	35.0	41.0
37	38.91171244351932	40.0	39.0	41.0	35.0	41.0
38	38.859369447123925	40.0	38.0	41.0	35.0	41.0
39	38.74650962075443	40.0	38.0	41.0	35.0	41.0
40	38.66873127887496	40.0	38.0	41.0	35.0	41.0
41	38.60460983906179	40.0	38.0	41.0	35.0	41.0
42	38.533126872112504	40.0	38.0	41.0	35.0	41.0
43	37.793115702898916	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	5.0
23	6.0
24	13.0
25	24.0
26	23.0
27	36.0
28	42.0
29	70.0
30	92.0
31	136.0
32	149.0
33	204.0
34	318.0
35	549.0
36	803.0
37	1729.0
38	4678.0
39	10817.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.05716606589836	16.647205158145912	13.560440676245115	27.735188099710616
2	21.866274051886073	19.52073919886277	30.72041427628573	27.892572472965426
3	21.76473574656039	19.282124181347413	29.33441640859014	29.618723663502056
4	15.306899527846879	16.048129156724375	34.43163933593949	34.21333197948926
5	17.144742854241763	33.07102604457531	32.47702695842006	17.30720414276286
6	33.43148702848149	34.929177032035334	15.245976544651471	16.3933593948317
7	28.857186373559422	27.34426562420673	19.810123369040973	23.988424633192874
8	27.212265827283343	29.897954003147685	20.840737168096666	22.049043001472306
9	27.643803624917503	12.037366096359852	17.34781946489313	42.971010813829515
10	19.91673858963294	22.871503274610347	28.110879829415648	29.100878306341066
11	40.68132202873534	18.231202721226584	18.424125501345383	22.663349748692692
12	22.587196019698432	24.303193379702492	26.455805452606995	26.65380514799208
13	37.21886581712951	16.63197441234706	19.535969944661623	26.613189825861806
14	24.023963040056863	19.596892927857034	23.023810732598875	33.355333299487235
15	29.72026196882774	24.39457785449561	18.886124790577245	26.999035386099408
16	24.958115449053157	23.68380971721582	22.856272528811495	28.501802304919533
17	27.99411077829111	23.252271919581663	20.830583337564097	27.923033964563132
18	26.52180535106869	20.820429507031527	22.257196527389958	30.400568614509826
19	29.146570543737628	22.170888967863124	21.845966390820937	26.836574097578314
20	31.37533634563639	20.307661065136823	21.0539676092806	27.263034979946184
21	28.999340001015383	20.744275778037263	20.805198761232674	29.45118545971468
22	29.13133979793877	22.693811240290398	20.51073767578819	27.664111285982635
23	29.298878001726152	20.43966086206021	21.82058181448952	28.440879321724122
24	27.867187896634004	21.07935218561202	23.099964461593135	27.953495456160837
25	28.943493933086256	22.511042290704168	21.444890084784486	27.10057369142509
26	28.984109255216527	22.42981164644362	22.09473523886886	26.491343859470984
27	26.44565162207443	22.15565822206427	24.39965476976189	26.999035386099408
28	26.095344468700816	22.866426359344064	21.54642839011017	29.49180078184495
29	26.98380464030055	24.206731989643092	21.5362745595776	27.273188810478754
30	27.466111590597553	21.46012083058334	23.907193988932324	27.166573589886784
31	28.15149515154592	21.972889272478042	22.617657511296134	27.257958064679897
32	25.719652738995784	21.515966898512463	22.734426562420673	30.029953800071073
33	25.882114027516877	21.312890287861094	24.01888612479058	28.786109559831445
34	25.94303701071229	21.622582119104433	24.06965527745342	28.364725592729854
35	25.43026856881759	23.084733715794282	24.775346499466924	26.709651215921205
36	26.491343859470984	21.769812661826673	25.73488348479464	26.0039599939077
37	27.12088135249023	20.769660354368686	24.425039346093314	27.684418947047774
38	25.415037823018732	19.67812357211758	26.3898055541453	28.517033050718382
39	24.968269279585723	19.200893537086866	27.090419860892524	28.74041732243489
40	24.13057826064883	19.972584657562066	29.405493222318118	26.491343859470984
41	22.399350154845916	19.541046859927906	28.938417017819972	29.121185967406205
42	19.744123470579275	20.58181448951617	32.16733512717673	27.506726912727824
43	19.50043153779763	21.20627506726913	29.958876986343096	29.33441640859014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.5
7	1.0
8	1.5
9	2.0
10	1.5
11	1.0
12	1.0
13	5.0
14	9.0
15	14.5
16	20.0
17	17.0
18	14.0
19	14.0
20	24.0
21	34.0
22	31.5
23	29.0
24	26.5
25	24.0
26	24.0
27	36.5
28	49.0
29	56.5
30	64.0
31	73.0
32	82.0
33	82.0
34	129.0
35	176.0
36	182.0
37	188.0
38	258.5
39	329.0
40	329.0
41	401.0
42	473.0
43	591.0
44	709.0
45	1033.5
46	1358.0
47	1358.0
48	1613.0
49	1868.0
50	2072.0
51	2276.0
52	2368.0
53	2460.0
54	2460.0
55	2291.0
56	2122.0
57	1998.0
58	1874.0
59	1802.0
60	1730.0
61	1730.0
62	1580.0
63	1430.0
64	1160.5
65	891.0
66	742.0
67	593.0
68	593.0
69	488.0
70	383.0
71	319.5
72	256.0
73	190.0
74	124.0
75	124.0
76	90.0
77	56.0
78	48.5
79	41.0
80	26.0
81	11.0
82	11.0
83	8.5
84	6.0
85	5.5
86	5.0
87	5.0
88	5.0
89	5.0
90	3.0
91	1.0
92	0.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	19697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.56374067116819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.76045908922622	59.48621617505204
2	6.345797852647168	8.701832766411128
3	2.502776749352092	5.147992080012185
4	1.310625694187338	3.5944560085292174
5	0.7330618289522399	2.513073056810682
6	0.3998519067012218	1.6449205462760828
7	0.3628285820066642	1.7413819363354825
8	0.2665679378008145	1.4621515966898513
9	0.20733061828952243	1.2793826471036198
>10	1.0884857460199926	13.296441082398335
>50	0.022213994816734542	1.1321521043813778
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	87	0.4416916281667259	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	78	0.39599939077016805	TruSeq Adapter, Index 11 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	58	0.29446108544448396	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	48	0.24369193278164186	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	47	0.23861501751535766	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	44	0.22338427171650507	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	41	0.20815352591765243	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	40	0.20307661065136823	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	40	0.20307661065136823	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	39	0.19799969538508402	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	38	0.19292278011879982	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	38	0.19292278011879982	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	36	0.1827689495862314	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	36	0.1827689495862314	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	35	0.1776920343199472	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	35	0.1776920343199472	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	34	0.17261511905366297	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	34	0.17261511905366297	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	34	0.17261511905366297	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	33	0.1675382037873788	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	31	0.15738437325481036	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	30	0.15230745798852616	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	30	0.15230745798852616	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	30	0.15230745798852616	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	29	0.14723054272224198	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	28	0.14215362745595778	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	27	0.13707671218967354	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	27	0.13707671218967354	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	26	0.13199979692338934	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	26	0.13199979692338934	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	26	0.13199979692338934	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	26	0.13199979692338934	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	25	0.12692288165710516	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	24	0.12184596639082093	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	24	0.12184596639082093	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	24	0.12184596639082093	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	24	0.12184596639082093	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	23	0.11676905112453673	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	22	0.11169213585825254	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	22	0.11169213585825254	RNA PCR Primer, Index 11 (95% over 22bp)
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	21	0.10661522059196832	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	20	0.10153830532568411	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	20	0.10153830532568411	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.010153830532568412	0.0
25	0.0	0.0	0.0	0.010153830532568412	0.0
26	0.0	0.0	0.0	0.010153830532568412	0.0
27	0.0	0.0	0.0	0.05584606792912626	0.0
28	0.0	0.0	0.0	0.23861501751535766	0.0
29	0.0	0.0	0.0	0.5229222724272732	0.0
30	0.0	0.0	0.0	0.863075595268315	0.0
31	0.0	0.0	0.0	1.7210742752703458	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	7.903722E-6	37.0	1
TCACTAA	25	0.005367502	29.6	33
GTATCAA	45	1.259735E-4	24.666666	2
CCGTGCC	55	1.7823802E-5	23.545454	9
AACTCCG	55	1.7823802E-5	23.545454	5
GCAGCCG	55	1.7823802E-5	23.545454	17
TAACTCC	55	1.7823802E-5	23.545454	4
CTAACTC	55	1.7823802E-5	23.545454	3
ATACGGA	55	1.7823802E-5	23.545454	29
GCTAACT	55	1.7823802E-5	23.545454	2
CGTGCCA	55	1.7823802E-5	23.545454	10
GGCTAAC	55	1.7823802E-5	23.545454	1
TCCGTGC	55	1.7823802E-5	23.545454	8
AATACGG	55	1.7823802E-5	23.545454	28
CTCCGTG	55	1.7823802E-5	23.545454	7
TACGGAG	60	3.4923352E-5	21.583334	30
GCGGTAA	60	3.4923352E-5	21.583334	23
CGCGGTA	60	3.4923352E-5	21.583334	22
AGCCGCG	60	3.4923352E-5	21.583334	19
TCTTATA	60	3.4923352E-5	21.583334	37
>>END_MODULE