FastQCFastQC Report
Fri 10 Feb 2017
ERR1632584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797827
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA25190.315732608698377No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17080.21408149887130917No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16940.21232673248711814No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15530.1946537281891939No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14370.18011423529161083No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14250.17861014981944706No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14020.1757273193311332No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13900.17422323385896943No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12320.15441944180881317No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11950.14978184493630825No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11700.14664833353596707No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11540.14464288623974872No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT11470.14376550304765318No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT11250.14100801301535293No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11170.1400052893672438No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC10990.13774916115899813No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10820.13561837340676614No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10500.13160747881432944No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC10150.12722056285385178No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC9830.12320966826141508No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9750.1222069446133059No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9290.11644128363667812No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8790.11017426083599577No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8320.10428325940335435No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8300.10403257849132706No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8040.10077372663497225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.028.4380151
ATACGGC1550.023.87096829
CGACGGT502.701099E-422.19999918
GCGGTAA9850.021.78680223
CTTTTTA855.1757524E-821.7647062
AAACACG1450.021.68965515
ATACACA1950.020.87179637
TTTTACG806.954633E-720.81254
GTATCAA16450.020.805472
CGCGGTA10450.020.7129222
TTAGCAT450.003824797720.5555554
CCGCTTA909.469113E-820.55555525
ATATAAG450.003824797720.5555551
CTTATTG2350.020.46808628
TTATTGA2350.020.46808629
AACGTCA5650.020.30088428
GGTAATA10700.020.22897125
AGCCGCG10800.020.21296319
CAAACGA555.141198E-420.18181823
AATAAAT555.141198E-420.18181834
Produced by FastQC (version 0.11.5)