##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632584.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797827
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07135130799033	34.0	33.0	34.0	31.0	34.0
2	33.21516694722039	34.0	33.0	34.0	31.0	34.0
3	33.28599182529546	34.0	34.0	34.0	31.0	34.0
4	36.5584042655864	37.0	37.0	37.0	35.0	37.0
5	36.53011116445044	37.0	37.0	37.0	35.0	37.0
6	36.57411443834315	37.0	37.0	37.0	35.0	37.0
7	36.57511841539582	37.0	37.0	37.0	35.0	37.0
8	36.55331168285856	37.0	37.0	37.0	35.0	37.0
9	38.342999171499585	39.0	39.0	39.0	37.0	39.0
10	38.387396014424176	39.0	39.0	39.0	37.0	39.0
11	38.44830019540577	39.0	39.0	39.0	37.0	39.0
12	38.413826556383775	39.0	39.0	39.0	37.0	39.0
13	38.44636493876492	39.0	39.0	39.0	37.0	39.0
14	40.00324381100163	41.0	40.0	41.0	38.0	41.0
15	40.006112854039785	41.0	40.0	41.0	38.0	41.0
16	39.96236151446366	41.0	40.0	41.0	38.0	41.0
17	39.96361617242836	41.0	40.0	41.0	38.0	41.0
18	39.962421677882546	41.0	40.0	41.0	38.0	41.0
19	39.95999759346324	41.0	40.0	41.0	38.0	41.0
20	39.94571254169137	41.0	40.0	41.0	38.0	41.0
21	39.9094490409575	41.0	40.0	41.0	38.0	41.0
22	39.880594414578596	41.0	40.0	41.0	38.0	41.0
23	39.84731777691154	41.0	40.0	41.0	38.0	41.0
24	39.824975840627104	41.0	40.0	41.0	38.0	41.0
25	39.78904699891079	41.0	40.0	41.0	38.0	41.0
26	39.709824310282805	41.0	40.0	41.0	38.0	41.0
27	39.62012817315032	41.0	40.0	41.0	37.0	41.0
28	39.58446505320076	41.0	40.0	41.0	37.0	41.0
29	39.554335714384194	41.0	40.0	41.0	37.0	41.0
30	39.50731925592892	41.0	40.0	41.0	37.0	41.0
31	39.44190031172172	41.0	39.0	41.0	37.0	41.0
32	39.39474848557394	41.0	39.0	41.0	36.0	41.0
33	39.33059046635424	41.0	39.0	41.0	36.0	41.0
34	39.29465535761512	41.0	39.0	41.0	36.0	41.0
35	39.21423065401397	41.0	39.0	41.0	35.0	41.0
36	39.17125642526513	41.0	39.0	41.0	35.0	41.0
37	39.11361861656725	41.0	39.0	41.0	35.0	41.0
38	39.03975423243385	41.0	39.0	41.0	35.0	41.0
39	38.9358858499399	41.0	39.0	41.0	35.0	41.0
40	38.871815569039406	40.0	38.0	41.0	35.0	41.0
41	38.81264359315992	40.0	38.0	41.0	35.0	41.0
42	38.74055152307455	40.0	38.0	41.0	35.0	41.0
43	38.01774444835785	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	3.0
18	3.0
19	9.0
20	29.0
21	59.0
22	110.0
23	254.0
24	392.0
25	602.0
26	926.0
27	1183.0
28	1693.0
29	2292.0
30	3148.0
31	4183.0
32	5640.0
33	7730.0
34	11693.0
35	18689.0
36	30034.0
37	58324.0
38	151623.0
39	499207.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.045046106486744	15.768080047428828	12.61250872682925	28.57436511925518
2	21.765871548593868	18.783395397749135	31.58303742540676	27.86769562825023
3	21.811746155494863	18.686757906162615	28.662730140744795	30.838765797597723
4	16.025404003624846	15.572172914679497	33.536217751467426	34.86620533022823
5	17.87342368709006	32.16023022534961	32.27830093491446	17.688045152645874
6	34.189366867754536	33.012921347610444	15.427028666615694	17.37068311801932
7	30.31760018149298	26.98605086065024	19.25104063913605	23.445308318720727
8	27.406693431032043	30.03784028367052	20.197987784319157	22.35747850097828
9	28.201477262614578	12.689718447733656	17.118372780063847	41.990431509587914
10	20.141459238657003	22.399592894198868	28.54754226166826	28.911405605475878
11	40.0698397020908	18.936561434997813	18.182638592075726	22.810960270835658
12	22.80594665259511	23.089592104553994	26.179986388026478	27.924474854824417
13	35.98875445428645	17.11737005641574	20.050462067591095	26.843413421706714
14	24.28521471446817	19.751149058630503	22.6175599472066	33.34607627969472
15	30.448580958027243	23.226463882520896	19.392926035343503	26.932029124108357
16	25.803338317705464	23.48052898686056	22.12885750920939	28.58727518622458
17	27.47349989408732	23.05136326546983	21.119114795563448	28.356022044879403
18	27.006356014524453	20.697594841989552	22.733249188107195	29.5627999553788
19	28.628888217621114	21.926558013203366	22.53094969210117	26.913604077074353
20	30.56777973169622	20.037050638797634	21.99198573124249	27.403183898263663
21	28.100703535979605	21.39586652244158	21.28694566616572	29.21648427541309
22	29.07560160285375	22.307718339940866	20.563605894510967	28.05307416269442
23	29.039252870609793	21.398122650649828	21.5714685013167	27.991155977423677
24	28.548795666228393	20.746477619834877	22.158939218652666	28.545787495284063
25	28.920680799220882	21.52785002262395	22.235898258645044	27.31557091951012
26	28.587149845768568	22.152797536307997	21.843206609954287	27.41684600796915
27	27.602475223325357	21.22878769457539	23.8877601284489	27.280976953650356
28	26.816715904575805	22.742900403220247	21.425572210516815	29.01481148168713
29	27.9362568576897	23.22859467027313	21.451016323087586	27.384132148949586
30	27.428628010834426	21.60618780763248	23.247646419587202	27.717537761945888
31	28.434109148975907	21.69217136045784	22.232514066332676	27.641205424233572
32	26.09801372979355	21.604057019880248	22.50425217497026	29.793677075355934
33	26.575936888573587	21.268645959587733	23.920223306556434	28.235193845282247
34	26.716819561132926	21.089534447944228	23.65888845576798	28.534757535154863
35	26.106662221258492	22.561407422912485	24.242598959423532	27.089331396405488
36	26.59461261651962	20.860161413439254	24.919061400529188	27.626164569511936
37	26.79691211252565	20.54179665516459	24.70497990165788	27.956311330651886
38	25.75608496578832	19.7520264418226	26.24516342515357	28.246725167235503
39	25.069219266833535	18.64702498160629	27.64734710657824	28.63640864498193
40	24.75837493591969	19.772582276608837	28.63941681592626	26.829625971545212
41	22.887793970372023	19.138986271459853	29.536729140527957	28.436490617640164
42	21.110967665922562	19.84101816559229	30.832248093885017	28.215766074600136
43	20.22142644959371	20.68167660407582	30.171578550237083	28.92531839609339
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	3.0
3	15.0
4	27.0
5	27.0
6	38.5
7	50.0
8	47.0
9	44.0
10	69.0
11	94.0
12	94.0
13	209.0
14	324.0
15	589.5
16	855.0
17	824.0
18	793.0
19	793.0
20	961.5
21	1130.0
22	967.5
23	805.0
24	884.0
25	963.0
26	963.0
27	1100.5
28	1238.0
29	1597.5
30	1957.0
31	2487.5
32	3018.0
33	3018.0
34	4167.5
35	5317.0
36	6053.5
37	6790.0
38	9726.0
39	12662.0
40	12662.0
41	15955.0
42	19248.0
43	23634.5
44	28021.0
45	41598.5
46	55176.0
47	55176.0
48	64486.5
49	73797.0
50	82899.0
51	92001.0
52	95453.5
53	98906.0
54	98906.0
55	91682.5
56	84459.0
57	80903.5
58	77348.0
59	72227.5
60	67107.0
61	67107.0
62	61324.0
63	55541.0
64	45557.0
65	35573.0
66	30731.0
67	25889.0
68	25889.0
69	22024.0
70	18159.0
71	15294.0
72	12429.0
73	10071.0
74	7713.0
75	7713.0
76	6471.0
77	5229.0
78	3912.0
79	2595.0
80	1968.0
81	1341.0
82	1341.0
83	1002.0
84	663.0
85	492.0
86	321.0
87	230.5
88	140.0
89	140.0
90	102.5
91	65.0
92	45.5
93	26.0
94	15.0
95	4.0
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	797827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.9278242495434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.45706186766861	37.53942794605304
2	6.981061177321967	6.133256569454199
3	2.126115461641294	2.801868789996468
4	1.1039379021221285	1.9397436058732205
5	0.7222723213833707	1.5863925797019218
6	0.47349734048661013	1.2479824773313215
7	0.37443642179502806	1.1513724130467926
8	0.30539209869075745	1.0732168350789446
9	0.21819431477370063	0.8626321360465806
>10	1.8114489279937418	16.154990651150822
>50	0.24224594020470394	7.449817173003144
>100	0.16484188165304278	14.634543361207758
>500	0.014047395132170779	4.211701917856658
>1k	0.005446949132882546	3.2130535441991013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2519	0.315732608698377	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1708	0.21408149887130917	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1694	0.21232673248711814	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1553	0.1946537281891939	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1437	0.18011423529161083	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1425	0.17861014981944706	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1402	0.1757273193311332	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1390	0.17422323385896943	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1232	0.15441944180881317	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1195	0.14978184493630825	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1170	0.14664833353596707	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1154	0.14464288623974872	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1147	0.14376550304765318	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1125	0.14100801301535293	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1117	0.1400052893672438	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	1099	0.13774916115899813	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1082	0.13561837340676614	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1050	0.13160747881432944	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	1015	0.12722056285385178	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	983	0.12320966826141508	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	975	0.1222069446133059	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	929	0.11644128363667812	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	879	0.11017426083599577	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	832	0.10428325940335435	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	830	0.10403257849132706	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	804	0.10077372663497225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
3	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
4	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
5	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
6	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
7	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
8	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
9	0.0	1.2534045601364708E-4	0.0	1.2534045601364708E-4	0.0
10	1.2534045601364708E-4	1.2534045601364708E-4	0.0	2.5068091202729416E-4	0.0
11	1.2534045601364708E-4	1.2534045601364708E-4	0.0	2.5068091202729416E-4	0.0
12	1.2534045601364708E-4	1.2534045601364708E-4	0.0	2.5068091202729416E-4	0.0
13	1.2534045601364708E-4	1.2534045601364708E-4	0.0	2.5068091202729416E-4	0.0
14	1.2534045601364708E-4	1.2534045601364708E-4	0.0	3.760213680409412E-4	0.0
15	1.2534045601364708E-4	1.2534045601364708E-4	0.0	6.267022800682353E-4	0.0
16	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.0010027236481091766	0.0
17	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.001629425928177412	0.0
18	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.0022561282082456473	0.0
19	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.0028828304883138827	0.0
20	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.003509532768382118	0.0
21	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.007019065536764236	0.0
22	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.01027791739311906	0.0
23	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.012534045601364707	0.0
24	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.01717164247386965	0.0
25	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.018299706577992472	0.0
26	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.023187984362524706	0.0
27	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.05728058839823671	0.0
28	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.20568368831839484	0.0
29	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.42678425272646825	0.0
30	1.2534045601364708E-4	1.2534045601364708E-4	0.0	0.7332416676798353	0.0
31	1.2534045601364708E-4	1.2534045601364708E-4	0.0	1.5227612001097983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1210	0.0	28.438015	1
ATACGGC	155	0.0	23.870968	29
CGACGGT	50	2.701099E-4	22.199999	18
GCGGTAA	985	0.0	21.786802	23
CTTTTTA	85	5.1757524E-8	21.764706	2
AAACACG	145	0.0	21.689655	15
ATACACA	195	0.0	20.871796	37
TTTTACG	80	6.954633E-7	20.8125	4
GTATCAA	1645	0.0	20.80547	2
CGCGGTA	1045	0.0	20.71292	22
TTAGCAT	45	0.0038247977	20.555555	4
CCGCTTA	90	9.469113E-8	20.555555	25
ATATAAG	45	0.0038247977	20.555555	1
CTTATTG	235	0.0	20.468086	28
TTATTGA	235	0.0	20.468086	29
AACGTCA	565	0.0	20.300884	28
GGTAATA	1070	0.0	20.228971	25
AGCCGCG	1080	0.0	20.212963	19
CAAACGA	55	5.141198E-4	20.181818	23
AATAAAT	55	5.141198E-4	20.181818	34
>>END_MODULE