FastQCFastQC Report
Fri 10 Feb 2017
ERR1632583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315449
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA22950.1744651446008169No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18270.13888793864300328No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17110.1300696568243999No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15420.11722233245074495No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15320.11646213574224466No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15150.11516980133779416No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13950.10604744083579068No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13590.10331073268518962No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13560.10308267367263953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.028.3850751
GTAAGAC451.3233545E-424.6666663
GTATATC1107.4578566E-1121.8636361
CGAAATA450.003826098520.55555524
GACGGAT450.003826098520.5555557
ATACGGC2000.020.3529
CGGTAGC656.903101E-519.92307730
GTATCAA24100.019.4211622
GCGGTAA10600.019.37264323
CTTATAC18800.018.6968137
CGCGGTA10950.018.58447522
TACTTAC500.00703540918.57
GGTAATA11300.018.17256725
GCAGTCG2350.018.1063849
GTAACGT7000.017.97142826
CGGTAAT11550.017.7792224
TCTTATA35200.017.6590937
TAATACG10950.017.57077627
CAGTACT852.723599E-517.4117644
GGTAACG7450.017.3825525
Produced by FastQC (version 0.11.5)