##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1315449
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.998972214050106	34.0	33.0	34.0	31.0	34.0
2	33.138963198117146	34.0	33.0	34.0	31.0	34.0
3	33.21035555160253	34.0	33.0	34.0	31.0	34.0
4	36.5214949420312	37.0	37.0	37.0	35.0	37.0
5	36.481169547432096	37.0	37.0	37.0	35.0	37.0
6	36.52299557033378	37.0	37.0	37.0	35.0	37.0
7	36.52601811244678	37.0	37.0	37.0	35.0	37.0
8	36.49704169450887	37.0	37.0	37.0	35.0	37.0
9	38.27293418444957	39.0	39.0	39.0	37.0	39.0
10	38.301850546847504	39.0	39.0	39.0	37.0	39.0
11	38.37211324802406	39.0	39.0	39.0	37.0	39.0
12	38.334937348388266	39.0	39.0	39.0	37.0	39.0
13	38.366620066608434	39.0	39.0	39.0	37.0	39.0
14	39.90821993098934	41.0	40.0	41.0	38.0	41.0
15	39.8999436694239	41.0	40.0	41.0	38.0	41.0
16	39.86218089792915	41.0	40.0	41.0	38.0	41.0
17	39.86196500206393	41.0	40.0	41.0	38.0	41.0
18	39.85588798957618	41.0	40.0	41.0	38.0	41.0
19	39.8687588800478	41.0	40.0	41.0	38.0	41.0
20	39.84175213178162	41.0	40.0	41.0	38.0	41.0
21	39.80863948355277	41.0	40.0	41.0	38.0	41.0
22	39.78581153659321	41.0	40.0	41.0	38.0	41.0
23	39.74443859092979	41.0	40.0	41.0	38.0	41.0
24	39.72694570447049	41.0	40.0	41.0	38.0	41.0
25	39.68986178863643	41.0	40.0	41.0	38.0	41.0
26	39.60481782265979	41.0	40.0	41.0	37.0	41.0
27	39.532168864015254	41.0	40.0	41.0	37.0	41.0
28	39.495733395973545	41.0	40.0	41.0	37.0	41.0
29	39.45801091490434	41.0	39.0	41.0	37.0	41.0
30	39.41080269930647	41.0	39.0	41.0	37.0	41.0
31	39.35676335608602	41.0	39.0	41.0	36.0	41.0
32	39.30790779422083	41.0	39.0	41.0	36.0	41.0
33	39.24831597424149	41.0	39.0	41.0	36.0	41.0
34	39.211747471775794	41.0	39.0	41.0	36.0	41.0
35	39.137792495186055	41.0	39.0	41.0	35.0	41.0
36	39.09951507051965	40.0	39.0	41.0	35.0	41.0
37	39.049890189585454	40.0	39.0	41.0	35.0	41.0
38	38.98234443144508	40.0	39.0	41.0	35.0	41.0
39	38.912071087514605	40.0	39.0	41.0	35.0	41.0
40	38.84782838407266	40.0	39.0	41.0	35.0	41.0
41	38.79749728039628	40.0	38.0	41.0	35.0	41.0
42	38.736214022740526	40.0	38.0	41.0	35.0	41.0
43	38.0098377056047	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	2.0
14	0.0
15	0.0
16	6.0
17	4.0
18	20.0
19	51.0
20	73.0
21	156.0
22	283.0
23	487.0
24	744.0
25	1154.0
26	1634.0
27	2254.0
28	3106.0
29	4234.0
30	5851.0
31	7659.0
32	10135.0
33	13763.0
34	20092.0
35	31807.0
36	51406.0
37	98306.0
38	272980.0
39	789240.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.99474932133439	16.37836206496793	12.901602418641847	27.72528619505583
2	20.76264454190166	19.37125650633358	33.06886089844608	26.79723805331868
3	21.19694492146788	19.742384539423423	29.068173680621594	29.9924968584871
4	16.087510804295718	15.602733363285084	32.96440987069814	35.34534596172105
5	17.117197245959364	32.997326388176205	33.13271742196011	16.752758943904325
6	35.013368059118974	32.95574362822124	15.177555344220869	16.853332968438913
7	30.458345401456082	27.7651965222521	19.700345661443354	22.076112414848467
8	27.225532878887744	31.354313242094523	20.094963772825857	21.325190106191876
9	27.79423603651681	13.395198141471088	17.660965951549624	41.14959987046248
10	19.17200894903565	23.9901356875105	29.9915846224369	26.846270741016948
11	38.71909895404535	19.73911569357687	18.98112355553123	22.560661796846553
12	22.928216905406444	23.77902906155997	26.618211728466857	26.674542304566728
13	34.30813357264326	17.86264613831475	21.418694301337414	26.41052598770458
14	24.342486861900387	19.825093941308253	22.870670014572973	32.96174918221839
15	29.107855948805312	24.385894093955752	20.259242281532767	26.247007675706165
16	26.092079586513805	24.034455155616065	22.27612016885489	27.597345089015235
17	26.739919221497754	23.847598804666696	22.046920861242057	27.365561112593497
18	26.147421906892625	21.98709338028308	23.697764033421286	28.167720679403
19	28.18474908567341	22.518242820512235	23.33697467556705	25.960033418247306
20	29.29532045712148	21.30785762123807	23.15627591795653	26.240546003683914
21	27.206984079200335	22.734670823422267	21.917079263430207	28.141265833947195
22	27.856876245297236	22.96622674083146	21.689780447588618	27.487116566282694
23	27.569901987838374	22.653937932979538	22.590765586503164	27.185394492678927
24	27.908341562462702	22.100058611166226	22.701754305944206	27.289845520426866
25	28.000021285507838	22.37144883610083	23.004236576256474	26.62429330213486
26	27.72635047044773	22.75337166245138	22.539224249666844	26.981053617434046
27	27.05236006869137	22.020313976444545	23.82806174925824	27.099264205605845
28	26.315805477825442	22.964706347414456	22.57206474747406	28.147423427286043
29	26.93703822801188	23.582062094387542	22.561345973884205	26.919553703716375
30	26.65538534751252	22.97230831449946	23.65283640794892	26.719469930039097
31	27.5094663495126	22.513225522236134	22.984623501177165	26.992684627074105
32	25.09014032471042	22.63097999238283	23.541315550811927	28.73756413209482
33	25.96459459849831	22.00617431766644	24.510718393491498	27.518512690343755
34	26.124007848270818	21.604182298211487	24.56073933691082	27.711070516606878
35	25.756224680698374	22.652797637916787	24.736420796245238	26.854556885139598
36	25.77112453618498	21.558342436688918	25.644855862903082	27.025677164223016
37	26.23910162993776	20.75549869284176	25.248945417116133	27.756454260104345
38	25.00081721146164	20.44138541288944	26.993596863124303	27.56420051252462
39	24.424055968722467	19.39976388290234	28.17950372838476	27.996676419990436
40	23.9860306252846	20.12803232964562	29.251077008686764	26.634860036383014
41	22.491863994727275	19.76055324075658	30.16376917691222	27.583813587603927
42	21.348604164813686	20.189380204021592	30.680550899350717	27.781464731814005
43	20.336554286787248	20.87735822521436	30.566369353733975	28.219718134264422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	30.0
4	44.0
5	44.0
6	60.5
7	77.0
8	92.0
9	107.0
10	154.0
11	201.0
12	201.0
13	360.0
14	519.0
15	1053.0
16	1587.0
17	1609.5
18	1632.0
19	1632.0
20	1897.5
21	2163.0
22	2139.0
23	2115.0
24	2637.5
25	3160.0
26	3160.0
27	3763.0
28	4366.0
29	6822.0
30	9278.0
31	10784.0
32	12290.0
33	12290.0
34	14931.0
35	17572.0
36	19757.0
37	21942.0
38	28896.0
39	35850.0
40	35850.0
41	42856.0
42	49862.0
43	54456.5
44	59051.0
45	75639.5
46	92228.0
47	92228.0
48	104244.5
49	116261.0
50	130004.0
51	143747.0
52	150407.5
53	157068.0
54	157068.0
55	143008.0
56	128948.0
57	121002.0
58	113056.0
59	104292.5
60	95529.0
61	95529.0
62	87469.5
63	79410.0
64	66220.5
65	53031.0
66	45842.5
67	38654.0
68	38654.0
69	32973.0
70	27292.0
71	23360.0
72	19428.0
73	15639.5
74	11851.0
75	11851.0
76	9509.5
77	7168.0
78	5830.5
79	4493.0
80	3529.5
81	2566.0
82	2566.0
83	2015.5
84	1465.0
85	1098.0
86	731.0
87	560.0
88	389.0
89	389.0
90	285.5
91	182.0
92	129.5
93	77.0
94	51.5
95	26.0
96	26.0
97	17.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1315449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.46723908964633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6635585758714	41.136798552804564
2	6.771052521445106	6.42806337847975
3	1.9564957313416238	2.7860835197239586
4	0.9781397960630381	1.857183822512885
5	0.6305286461428818	1.4964726999667588
6	0.42260963423395753	1.2036067529862746
7	0.3093290595466364	1.0278097498812946
8	0.23819322440262855	0.9045099785802677
9	0.19098947843508343	0.8159168912836461
>10	1.4365581888579886	13.788293146466431
>50	0.2209298824990629	7.303105401904807
>100	0.16713751844222446	15.518997798135484
>500	0.011099602750790988	3.6204196420106785
>1k	0.00337813996763204	2.1127386652632865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2295	0.1744651446008169	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1827	0.13888793864300328	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1711	0.1300696568243999	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1542	0.11722233245074495	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1532	0.11646213574224466	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1515	0.11516980133779416	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1395	0.10604744083579068	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1359	0.10331073268518962	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1356	0.10308267367263953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.601967085002915E-5	0.0	0.0	0.0	0.0
8	7.601967085002915E-5	0.0	0.0	0.0	0.0
9	7.601967085002915E-5	0.0	0.0	0.0	0.0
10	7.601967085002915E-5	0.0	0.0	0.0	0.0
11	7.601967085002915E-5	0.0	0.0	7.601967085002915E-5	0.0
12	7.601967085002915E-5	0.0	0.0	7.601967085002915E-5	0.0
13	7.601967085002915E-5	0.0	0.0	7.601967085002915E-5	0.0
14	7.601967085002915E-5	0.0	0.0	2.2805901255008745E-4	0.0
15	7.601967085002915E-5	7.601967085002915E-5	0.0	2.2805901255008745E-4	0.0
16	7.601967085002915E-5	7.601967085002915E-5	0.0	6.841770376502624E-4	0.0
17	7.601967085002915E-5	7.601967085002915E-5	0.0	0.0015964130878506123	0.0
18	7.601967085002915E-5	7.601967085002915E-5	0.0	0.0016724327587006413	0.0
19	7.601967085002915E-5	7.601967085002915E-5	0.0	0.001900491771250729	0.0
20	7.601967085002915E-5	7.601967085002915E-5	0.0	0.002964767163151137	0.0
21	7.601967085002915E-5	7.601967085002915E-5	0.0	0.0045611802510017495	0.0
22	7.601967085002915E-5	7.601967085002915E-5	0.0	0.007069829389052712	0.0
23	7.601967085002915E-5	7.601967085002915E-5	0.0	0.00889430148945341	0.0
24	7.601967085002915E-5	7.601967085002915E-5	0.0	0.011783048981754519	0.0
25	7.601967085002915E-5	7.601967085002915E-5	0.0	0.01429169811980548	0.0
26	7.601967085002915E-5	7.601967085002915E-5	0.0	0.01999317343355767	0.0
27	7.601967085002915E-5	7.601967085002915E-5	0.0	0.05290969091162029	0.0
28	7.601967085002915E-5	7.601967085002915E-5	0.0	0.1885287837080723	0.0
29	7.601967085002915E-5	7.601967085002915E-5	0.0	0.40229609813835426	0.0
30	7.601967085002915E-5	7.601967085002915E-5	0.0	0.6828847032458119	0.0
31	7.601967085002915E-5	7.601967085002915E-5	0.0	1.478430558691367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1675	0.0	28.385075	1
GTAAGAC	45	1.3233545E-4	24.666666	3
GTATATC	110	7.4578566E-11	21.863636	1
CGAAATA	45	0.0038260985	20.555555	24
GACGGAT	45	0.0038260985	20.555555	7
ATACGGC	200	0.0	20.35	29
CGGTAGC	65	6.903101E-5	19.923077	30
GTATCAA	2410	0.0	19.421162	2
GCGGTAA	1060	0.0	19.372643	23
CTTATAC	1880	0.0	18.69681	37
CGCGGTA	1095	0.0	18.584475	22
TACTTAC	50	0.007035409	18.5	7
GGTAATA	1130	0.0	18.172567	25
GCAGTCG	235	0.0	18.106384	9
GTAACGT	700	0.0	17.971428	26
CGGTAAT	1155	0.0	17.77922	24
TCTTATA	3520	0.0	17.65909	37
TAATACG	1095	0.0	17.570776	27
CAGTACT	85	2.723599E-5	17.411764	4
GGTAACG	745	0.0	17.38255	25
>>END_MODULE