FastQCFastQC Report
Fri 10 Feb 2017
ERR1632582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21639
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT940.434400850316558RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA540.2495494246499376No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA430.198715282591617No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA380.17560885438328944No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA360.1663662830999584No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC320.14788114053329637No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA320.14788114053329637No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC310.14325985489163084RNA PCR Primer, Index 29 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT310.14325985489163084No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA300.13863856924996534No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA300.13863856924996534No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT300.13863856924996534No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA300.13863856924996534No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT290.13401728360829984No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA290.13401728360829984No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC270.1247747123249688RNA PCR Primer, Index 29 (95% over 24bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC270.1247747123249688No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT260.12015342668330328No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC260.12015342668330328No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT250.1155321410416378No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC250.1155321410416378No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA240.11091085539997228No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG240.11091085539997228No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.10628956975830677No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.10166828411664125No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG220.10166828411664125No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC220.10166828411664125No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC220.10166828411664125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCT250.00537925129.635
TGTCTCT1502.4337362E-411.137
CCTGTCT1500.0030837989.86666737
CTGTCTC2003.2473973E-49.2537
Produced by FastQC (version 0.11.5)