##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632582.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21639
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04062110079024	34.0	33.0	34.0	31.0	34.0
2	33.196358426914365	34.0	33.0	34.0	31.0	34.0
3	33.256065437404686	34.0	34.0	34.0	31.0	34.0
4	36.527242478857616	37.0	37.0	37.0	35.0	37.0
5	36.50427468921854	37.0	37.0	37.0	35.0	37.0
6	36.55196635704053	37.0	37.0	37.0	35.0	37.0
7	36.540829058644114	37.0	37.0	37.0	35.0	37.0
8	36.51980220897454	37.0	37.0	37.0	35.0	37.0
9	38.314940616479504	39.0	39.0	39.0	37.0	39.0
10	38.32917417625583	39.0	39.0	39.0	37.0	39.0
11	38.41919682055548	39.0	39.0	39.0	37.0	39.0
12	38.38712509820232	39.0	39.0	39.0	37.0	39.0
13	38.43338416747539	39.0	39.0	39.0	37.0	39.0
14	39.97120939045242	41.0	40.0	41.0	38.0	41.0
15	39.95836221636859	41.0	40.0	41.0	38.0	41.0
16	39.93682702527843	41.0	40.0	41.0	38.0	41.0
17	39.90785156430519	41.0	40.0	41.0	38.0	41.0
18	39.91214935995194	41.0	40.0	41.0	38.0	41.0
19	39.899071121586026	41.0	40.0	41.0	38.0	41.0
20	39.893802855954526	41.0	40.0	41.0	38.0	41.0
21	39.85965155506262	41.0	40.0	41.0	38.0	41.0
22	39.81112805582513	41.0	40.0	41.0	38.0	41.0
23	39.78390868339572	41.0	40.0	41.0	38.0	41.0
24	39.76352881371598	41.0	40.0	41.0	38.0	41.0
25	39.70433014464624	41.0	40.0	41.0	37.0	41.0
26	39.60862331900735	41.0	40.0	41.0	37.0	41.0
27	39.56051573547761	41.0	40.0	41.0	37.0	41.0
28	39.49664956790979	41.0	40.0	41.0	37.0	41.0
29	39.427792411848976	41.0	39.0	41.0	37.0	41.0
30	39.397430565183235	41.0	39.0	41.0	36.0	41.0
31	39.30472757521142	41.0	39.0	41.0	36.0	41.0
32	39.22829151069828	41.0	39.0	41.0	36.0	41.0
33	39.152456213318544	41.0	39.0	41.0	35.0	41.0
34	39.1506539119183	41.0	39.0	41.0	35.0	41.0
35	39.05513193770507	41.0	39.0	41.0	35.0	41.0
36	38.98438005453117	41.0	39.0	41.0	35.0	41.0
37	38.92910947825685	40.0	39.0	41.0	35.0	41.0
38	38.82850408983779	40.0	38.0	41.0	35.0	41.0
39	38.743564859743984	40.0	38.0	41.0	35.0	41.0
40	38.63547298858543	40.0	38.0	41.0	35.0	41.0
41	38.572484865289525	40.0	38.0	41.0	35.0	41.0
42	38.49216692083738	40.0	38.0	41.0	35.0	41.0
43	37.80646055732705	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	7.0
23	12.0
24	15.0
25	23.0
26	29.0
27	29.0
28	48.0
29	66.0
30	108.0
31	116.0
32	154.0
33	250.0
34	355.0
35	582.0
36	883.0
37	1733.0
38	4189.0
39	13036.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.635981330006004	15.7308563242294	13.01354036693008	28.619621978834513
2	21.761634086602893	18.660751421045337	30.726928231433988	28.850686260917787
3	21.475114376819633	18.753177133878644	28.346966125976248	31.424742363325475
4	16.317759600720922	15.18554461851287	32.612412773233515	35.884283007532694
5	18.318776283562087	31.25837608022552	32.9081750543001	17.51467258191229
6	36.22625814501595	31.748232358242063	14.996071907204584	17.02943758953741
7	30.38033180830907	27.08997643144323	19.455612551411804	23.074079208835897
8	28.259161698784602	29.79342853181755	20.084107398678313	21.863302370719534
9	28.13438698645963	12.14935995193863	16.37783631406257	43.33841674753916
10	20.2597162530616	22.658163501086	29.54850039280928	27.533619853043117
11	40.35306622302324	18.18938028559545	17.83816257682887	23.61939091455243
12	23.235824206294193	22.9400619252276	25.55108831276861	28.273025555709598
13	35.976708720366005	16.197606174037617	19.917741115578355	27.907943990018026
14	25.29691760247701	18.80863256157863	21.803225657377883	34.09122417856648
15	30.297148666759092	23.203475206802533	18.984241415961918	27.515134710476453
16	27.42733028328481	22.722861500069317	21.086926382919728	28.762881833726144
17	27.76006284948473	23.004759924210916	20.347520680253247	28.88765654605111
18	27.279449142751517	21.031470955219742	21.646101945561256	30.04297795646749
19	29.14182725634272	21.271777808586346	22.24686907897777	27.339525856093168
20	30.805490087342296	20.037894542261657	21.488978233744625	27.667637136651418
21	28.712047691667824	20.805027958778133	20.55085724848653	29.932067101067517
22	29.41448310920098	22.09436665280281	19.54341697860345	28.94773325939276
23	29.1972826840427	20.731087388511487	20.897453671611444	29.17417625583437
24	29.127963399417716	20.204260825361615	21.525948518877954	29.14182725634272
25	29.636304820000923	21.054577383428068	21.964970654836176	27.34414714173483
26	28.87841397476778	22.200656222561115	20.536993391561534	28.383936411109573
27	28.194463699801286	21.174730810111374	22.58884421646102	28.04196127362632
28	27.274827857109845	22.501039789269374	20.86048338647812	29.36364896714266
29	28.028097416701325	23.046351494985906	20.934423956744766	27.991127131568
30	27.894080133093023	21.10541152548639	22.810665927260963	28.18984241415962
31	29.003188687092752	21.317990665003002	21.114654096769723	28.564166551134523
32	26.99755071860992	21.30874809371967	21.244050094736355	30.449651092934054
33	26.835805721151623	21.165488238828043	23.355977632977496	28.64272840704284
34	27.505892139193122	21.193215952678035	22.325430934886086	28.975460973242757
35	26.8126992929433	22.283839364111095	23.98909376588567	26.914367577059938
36	27.358010998659825	21.183973381394704	23.60552705762743	27.85248856231804
37	28.004990988493	20.051758399186653	23.263551920144184	28.679698692176164
38	26.216553445168444	20.735708674153148	24.714635611627155	28.333102269051253
39	26.63709043856001	19.321595267803502	25.352373030176995	28.688941263459494
40	25.361615601460326	20.814270530061464	26.63709043856001	27.187023429918206
41	24.645316327002174	19.367808124220158	27.04838486066824	28.938490688109432
42	22.653542215444336	20.18115439715329	29.12334211377605	28.04196127362632
43	22.367022505661076	20.453810250011554	28.08355284440131	29.095614399926063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	4.0
15	8.5
16	13.0
17	11.0
18	9.0
19	9.0
20	20.5
21	32.0
22	20.0
23	8.0
24	10.5
25	13.0
26	13.0
27	23.0
28	33.0
29	38.5
30	44.0
31	66.0
32	88.0
33	88.0
34	121.0
35	154.0
36	180.0
37	206.0
38	284.5
39	363.0
40	363.0
41	445.0
42	527.0
43	653.5
44	780.0
45	1060.5
46	1341.0
47	1341.0
48	1521.5
49	1702.0
50	1957.5
51	2213.0
52	2320.0
53	2427.0
54	2427.0
55	2354.5
56	2282.0
57	2188.0
58	2094.0
59	1936.5
60	1779.0
61	1779.0
62	1708.0
63	1637.0
64	1387.0
65	1137.0
66	1007.0
67	877.0
68	877.0
69	745.0
70	613.0
71	546.5
72	480.0
73	391.0
74	302.0
75	302.0
76	246.5
77	191.0
78	155.0
79	119.0
80	97.5
81	76.0
82	76.0
83	65.5
84	55.0
85	38.0
86	21.0
87	16.0
88	11.0
89	11.0
90	7.5
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	21639.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.98599750450576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.86733897592892	69.18526734137437
2	5.468515517137884	8.419982439114563
3	1.8728615162974966	4.325523360598918
4	0.8704003841767214	2.6803456721659966
5	0.46221261780419	1.7791949720412217
6	0.3181463473197671	1.4695688340496327
7	0.22810492826700282	1.229261980683026
8	0.13206074794405429	0.81334627293313
9	0.10204694159313284	0.7070567031748233
>10	0.6663065009904556	8.706502148897824
>50	0.012005522540368569	0.6839502749664956
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	94	0.434400850316558	RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	54	0.2495494246499376	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	43	0.198715282591617	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	38	0.17560885438328944	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	36	0.1663662830999584	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	32	0.14788114053329637	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	32	0.14788114053329637	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	31	0.14325985489163084	RNA PCR Primer, Index 29 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	31	0.14325985489163084	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	30	0.13863856924996534	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	30	0.13863856924996534	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	30	0.13863856924996534	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	30	0.13863856924996534	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	29	0.13401728360829984	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	29	0.13401728360829984	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	27	0.1247747123249688	RNA PCR Primer, Index 29 (95% over 24bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	27	0.1247747123249688	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	26	0.12015342668330328	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	26	0.12015342668330328	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	25	0.1155321410416378	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	25	0.1155321410416378	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	24	0.11091085539997228	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	24	0.11091085539997228	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	23	0.10628956975830677	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	22	0.10166828411664125	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	22	0.10166828411664125	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	22	0.10166828411664125	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	22	0.10166828411664125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.02310642820832756	0.0
28	0.0	0.0	0.0	0.10628956975830677	0.0
29	0.0	0.0	0.0	0.2680345672165997	0.0
30	0.0	0.0	0.0	0.42053699339156153	0.0
31	0.0	0.0	0.0	0.8456952724247886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTCT	25	0.005379251	29.6	35
TGTCTCT	150	2.4337362E-4	11.1	37
CCTGTCT	150	0.003083798	9.866667	37
CTGTCTC	200	3.2473973E-4	9.25	37
>>END_MODULE