FastQCFastQC Report
Fri 10 Feb 2017
ERR1632581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343586
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4770.13882987083292103No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3940.11467289121209828No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3890.11321765147590412No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3730.10856088432008289No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3550.10332202126978399No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3510.10215782948082867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.033.1911741
GGACCGT303.5942727E-430.8333346
ATACTGC358.8566914E-426.428576
ACTATAC551.8976292E-523.54545435
TTATTAC400.001928447723.1251
CATACTG652.6737634E-622.769235
CCAATAC502.6967624E-422.1999993
ATGCCGT502.6967624E-422.19999934
GTATCAA5150.021.912622
CGAACTA450.003820432620.55555529
TTAGAAC450.003820432620.5555553
ATACGGC450.003820432620.55555529
CGTATGC555.1330123E-420.18181831
TCGTATG555.1330123E-420.18181830
GCCGTCT555.1330123E-420.18181836
TGCCGTC555.1330123E-420.18181835
ATACACA1202.3646862E-1020.04166837
CTTATAC4700.019.6808537
ATACCTT851.240116E-619.5882345
CTCGTAT609.2188566E-418.529
Produced by FastQC (version 0.11.5)