##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343586
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0889326107583	34.0	33.0	34.0	31.0	34.0
2	33.2204280733208	34.0	33.0	34.0	31.0	34.0
3	33.29479082384032	34.0	34.0	34.0	31.0	34.0
4	36.57146391296502	37.0	37.0	37.0	35.0	37.0
5	36.53960580466026	37.0	37.0	37.0	35.0	37.0
6	36.583714121064304	37.0	37.0	37.0	35.0	37.0
7	36.58007020076487	37.0	37.0	37.0	35.0	37.0
8	36.55393118462335	37.0	37.0	37.0	35.0	37.0
9	38.354321770968546	39.0	39.0	39.0	37.0	39.0
10	38.384078513094245	39.0	39.0	39.0	37.0	39.0
11	38.4520294773361	39.0	39.0	39.0	37.0	39.0
12	38.41629169989464	39.0	39.0	39.0	37.0	39.0
13	38.450734313970884	39.0	39.0	39.0	37.0	39.0
14	40.026435885047704	41.0	40.0	41.0	38.0	41.0
15	40.019674841233346	41.0	40.0	41.0	38.0	41.0
16	39.988806295949196	41.0	40.0	41.0	38.0	41.0
17	39.97967030088537	41.0	40.0	41.0	38.0	41.0
18	39.96957675807513	41.0	40.0	41.0	38.0	41.0
19	39.97768826436467	41.0	40.0	41.0	38.0	41.0
20	39.953091802343515	41.0	40.0	41.0	38.0	41.0
21	39.9234427479583	41.0	40.0	41.0	38.0	41.0
22	39.90685883592463	41.0	40.0	41.0	38.0	41.0
23	39.86735780852538	41.0	40.0	41.0	38.0	41.0
24	39.853119742946454	41.0	40.0	41.0	38.0	41.0
25	39.809057994213966	41.0	40.0	41.0	38.0	41.0
26	39.73061184099468	41.0	40.0	41.0	38.0	41.0
27	39.6560657302684	41.0	40.0	41.0	37.0	41.0
28	39.61727194938094	41.0	40.0	41.0	37.0	41.0
29	39.5809317026887	41.0	40.0	41.0	37.0	41.0
30	39.533691710372366	41.0	40.0	41.0	37.0	41.0
31	39.47694609209921	41.0	40.0	41.0	37.0	41.0
32	39.42538694824585	41.0	39.0	41.0	36.0	41.0
33	39.36351597562182	41.0	39.0	41.0	36.0	41.0
34	39.332816238147075	41.0	39.0	41.0	36.0	41.0
35	39.262516516971004	41.0	39.0	41.0	36.0	41.0
36	39.22029710174454	41.0	39.0	41.0	35.0	41.0
37	39.17205008353076	41.0	39.0	41.0	35.0	41.0
38	39.10751311171002	41.0	39.0	41.0	35.0	41.0
39	39.02863329704935	41.0	39.0	41.0	35.0	41.0
40	38.954867194821674	41.0	39.0	41.0	35.0	41.0
41	38.91189978637081	40.0	39.0	41.0	35.0	41.0
42	38.854473115901115	40.0	39.0	41.0	35.0	41.0
43	38.14391156799171	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	10.0
19	9.0
20	15.0
21	29.0
22	80.0
23	129.0
24	173.0
25	266.0
26	338.0
27	533.0
28	703.0
29	939.0
30	1379.0
31	1767.0
32	2320.0
33	3307.0
34	4917.0
35	7755.0
36	12535.0
37	23594.0
38	62690.0
39	220096.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.086156013341636	16.307998579686018	12.765654013842239	27.840191393130105
2	20.639374130494257	19.240597696064448	32.84010407874594	27.279924094695364
3	21.091371592556158	19.619251075422163	28.69121559085644	30.598161741165242
4	16.159855174541455	15.257606538101086	32.305157951720965	36.277380335636494
5	17.256814887684595	33.117181724517295	32.82584272933123	16.800160658466876
6	36.28902225352604	32.34386732870373	14.9668496388095	16.400260778960725
7	31.452387466311198	27.22229660114207	19.581996938175593	21.743318994371133
8	27.93769245545511	31.31588597905619	19.707438603435527	21.03898296205317
9	28.01743959299855	13.159150838509138	17.39448056672856	41.42892900176375
10	19.17336562025228	24.05453074339467	30.036439202994302	26.735664433358753
11	39.398578521825684	19.360800498274084	18.787727090160832	22.452893889739396
12	23.3007165600461	23.293440361365132	26.48798263025851	26.917860448330256
13	34.48452498064531	17.417473354560432	21.29190362820371	26.806098036590548
14	24.539998719389033	19.162596846204444	22.692426350316953	33.60497808408957
15	28.96596485304989	24.41077343081499	19.875664316939574	26.747597399195545
16	26.8125010914298	23.475927424283878	21.779699987775984	27.931871496510336
17	27.220259265511398	23.582742020920527	21.65484041841053	27.54215829515754
18	26.842479029995403	21.70926638454419	23.21718579918856	28.23106878627185
19	28.587020425744935	22.201719511272287	22.7145459943071	26.496714068675676
20	29.319588108945066	21.327411477766848	22.720366953251876	26.63263346003621
21	27.54361353489374	22.415348704545586	21.7558340561024	28.285203704458272
22	28.38095847909985	22.595216335939185	21.14841699021497	27.875408194746004
23	28.002596147689367	22.473849341940593	21.979358879581824	27.544195630788217
24	28.22874040269394	21.747684713579716	22.192114929013407	27.83145995471294
25	28.32624146501895	21.98052307137078	22.609477685353884	27.08375777825639
26	28.043633908250044	22.48490916393567	22.28495922418259	27.186497703631694
27	27.446985616410448	21.84373053616853	23.3111942861467	27.398089561274325
28	26.682693706961285	22.67350823374643	22.291944374916323	28.351853684375968
29	27.184460368001023	23.17352860710273	22.211615141478408	27.430395883417834
30	26.950748866368247	22.72735210398561	23.04575855826489	27.276140471381254
31	27.827094235504358	22.150495072558254	22.635672000605382	27.386738691332006
32	25.813042440611667	22.29019808723289	22.975907050927567	28.92085242122787
33	26.300547752236703	21.906887940719354	23.999813729313768	27.792750577730175
34	26.459168883481865	21.546279534090445	23.877573591473457	28.11697799095423
35	26.084008079491017	22.366452649409464	24.56328255516814	26.986256715931383
36	26.044425558666536	21.741281658740462	24.970458633355257	27.243834149237745
37	26.354973718370367	20.81516709062651	24.944846413998242	27.885012777004885
38	25.249282566810056	20.46998422520126	26.462079362954256	27.81865384503443
39	24.77400126896905	19.50865285547141	27.663816337103377	28.053529538456168
40	24.171240970237438	20.17224217517594	28.805888482068536	26.850628372518088
41	22.98580268113369	19.83404446048442	29.398753150594032	27.781399707787862
42	21.96451543427264	20.188249812274073	30.100760799334083	27.7464739541192
43	20.895787372011664	20.70020315146717	30.165955539515583	28.23805393700558
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	5.0
4	9.0
5	9.0
6	13.5
7	18.0
8	15.0
9	12.0
10	22.0
11	32.0
12	32.0
13	62.0
14	92.0
15	168.0
16	244.0
17	245.5
18	247.0
19	247.0
20	297.0
21	347.0
22	390.0
23	433.0
24	511.0
25	589.0
26	589.0
27	829.0
28	1069.0
29	1559.0
30	2049.0
31	2417.0
32	2785.0
33	2785.0
34	3499.5
35	4214.0
36	4862.0
37	5510.0
38	7234.0
39	8958.0
40	8958.0
41	10720.5
42	12483.0
43	13954.5
44	15426.0
45	19532.5
46	23639.0
47	23639.0
48	26564.5
49	29490.0
50	32890.0
51	36290.0
52	38052.0
53	39814.0
54	39814.0
55	36456.5
56	33099.0
57	31242.5
58	29386.0
59	27306.0
60	25226.0
61	25226.0
62	23358.5
63	21491.0
64	18546.0
65	15601.0
66	13607.5
67	11614.0
68	11614.0
69	9959.5
70	8305.0
71	7151.5
72	5998.0
73	4881.5
74	3765.0
75	3765.0
76	3037.5
77	2310.0
78	1833.0
79	1356.0
80	1079.5
81	803.0
82	803.0
83	627.5
84	452.0
85	356.0
86	260.0
87	174.5
88	89.0
89	89.0
90	72.0
91	55.0
92	36.0
93	17.0
94	11.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	343586.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.37250442356916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95474515882631	53.98002986333966
2	6.1560734949084415	7.556272955961704
3	1.8970989690696296	3.4928914461352303
4	0.9609869156189459	2.3591269491926337
5	0.5648747409858242	1.7333888769957493
6	0.3940000770975236	1.4508462884732616
7	0.2771909353849204	1.1908331335659015
8	0.22730266249158004	1.1160106927402837
9	0.1688354548813661	0.9325669231405755
>10	1.2163626314907168	14.535057607892751
>50	0.11693261451617219	4.91615819563659
>100	0.06559634472858197	6.736817066925741
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	477	0.13882987083292103	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	394	0.11467289121209828	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	389	0.11321765147590412	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	373	0.10856088432008289	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	355	0.10332202126978399	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	351	0.10215782948082867	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.910479472388281E-4
6	0.0	0.0	0.0	0.0	2.910479472388281E-4
7	0.0	0.0	0.0	0.0	2.910479472388281E-4
8	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
9	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
10	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
11	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
12	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
13	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
14	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
15	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
16	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
17	5.820958944776563E-4	0.0	0.0	0.0	2.910479472388281E-4
18	5.820958944776563E-4	0.0	0.0	2.910479472388281E-4	2.910479472388281E-4
19	5.820958944776563E-4	0.0	0.0	5.820958944776563E-4	2.910479472388281E-4
20	5.820958944776563E-4	0.0	0.0	8.731438417164843E-4	2.910479472388281E-4
21	5.820958944776563E-4	0.0	0.0	0.0034925753668659373	2.910479472388281E-4
22	5.820958944776563E-4	0.0	0.0	0.006985150733731875	2.910479472388281E-4
23	5.820958944776563E-4	0.0	0.0	0.007276198680970703	2.910479472388281E-4
24	5.820958944776563E-4	0.0	0.0	0.0093135343116425	2.910479472388281E-4
25	5.820958944776563E-4	0.0	0.0	0.010186678153358985	2.910479472388281E-4
26	5.820958944776563E-4	0.0	0.0	0.012806109678508438	2.910479472388281E-4
27	5.820958944776563E-4	0.0	0.0	0.041328808507913595	2.910479472388281E-4
28	5.820958944776563E-4	0.0	0.0	0.16153161071754962	2.910479472388281E-4
29	5.820958944776563E-4	0.0	0.0	0.34838439284487727	2.910479472388281E-4
30	5.820958944776563E-4	0.0	0.0	0.5861705657389998	2.910479472388281E-4
31	5.820958944776563E-4	0.0	0.0	1.2526703649159163	2.910479472388281E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	340	0.0	33.191174	1
GGACCGT	30	3.5942727E-4	30.833334	6
ATACTGC	35	8.8566914E-4	26.42857	6
ACTATAC	55	1.8976292E-5	23.545454	35
TTATTAC	40	0.0019284477	23.125	1
CATACTG	65	2.6737634E-6	22.76923	5
CCAATAC	50	2.6967624E-4	22.199999	3
ATGCCGT	50	2.6967624E-4	22.199999	34
GTATCAA	515	0.0	21.91262	2
CGAACTA	45	0.0038204326	20.555555	29
TTAGAAC	45	0.0038204326	20.555555	3
ATACGGC	45	0.0038204326	20.555555	29
CGTATGC	55	5.1330123E-4	20.181818	31
TCGTATG	55	5.1330123E-4	20.181818	30
GCCGTCT	55	5.1330123E-4	20.181818	36
TGCCGTC	55	5.1330123E-4	20.181818	35
ATACACA	120	2.3646862E-10	20.041668	37
CTTATAC	470	0.0	19.68085	37
ATACCTT	85	1.240116E-6	19.588234	5
CTCGTAT	60	9.2188566E-4	18.5	29
>>END_MODULE