FastQCFastQC Report
Fri 10 Feb 2017
ERR1632580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT210.4086398131932283TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA110.21404942595835766No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA110.21404942595835766No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA100.1945903872348706No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT90.17513134851138354No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC90.17513134851138354No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC90.17513134851138354RNA PCR Primer, Index 6 (95% over 22bp)
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA80.15567230978789648No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG80.15567230978789648No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT80.15567230978789648No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA70.13621327106440942No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT70.13621327106440942No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC70.13621327106440942No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA70.13621327106440942No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT70.13621327106440942No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC70.13621327106440942No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT60.11675423234092236No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT60.11675423234092236No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG60.11675423234092236No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC60.11675423234092236No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA60.11675423234092236No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT60.11675423234092236No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)