FastQCFastQC Report
Fri 10 Feb 2017
ERR1632579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5583
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT500.8955758552749419Illumina PCR Primer Index 10 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA180.32240730789897903No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC180.32240730789897903No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT160.28658427368798134No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC160.28658427368798134Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA140.2507612394769837No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA140.2507612394769837No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA140.2507612394769837No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA130.23284972237148488No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC120.21493820526598603No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG110.1970266881604872No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC100.17911517105498836No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT100.17911517105498836No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA100.17911517105498836No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA100.17911517105498836No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT90.16120365394948952No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG80.14329213684399067No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG80.14329213684399067No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC80.14329213684399067No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC80.14329213684399067No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT70.12538061973849185No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCG70.12538061973849185No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC70.12538061973849185No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT70.12538061973849185No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC70.12538061973849185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.12538061973849185No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA70.12538061973849185No Hit
CAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCT60.10746910263299302No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC60.10746910263299302No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACCTG60.10746910263299302No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA60.10746910263299302No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG60.10746910263299302No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT60.10746910263299302No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.10746910263299302No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT60.10746910263299302No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT60.10746910263299302No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA60.10746910263299302No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG60.10746910263299302No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG60.10746910263299302No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC60.10746910263299302No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG60.10746910263299302No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC60.10746910263299302No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA60.10746910263299302No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT60.10746910263299302No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)