##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632579.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5583
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97456564571019	34.0	33.0	34.0	31.0	34.0
2	33.0951101558302	34.0	33.0	34.0	31.0	34.0
3	33.19416084542361	34.0	33.0	34.0	31.0	34.0
4	36.48199892530897	37.0	37.0	37.0	35.0	37.0
5	36.43202579258463	37.0	37.0	37.0	35.0	37.0
6	36.500089557585525	37.0	37.0	37.0	35.0	37.0
7	36.53662905248075	37.0	37.0	37.0	35.0	37.0
8	36.489342647322225	37.0	37.0	37.0	35.0	37.0
9	38.28604692817482	39.0	39.0	39.0	37.0	39.0
10	38.30789897904352	39.0	39.0	39.0	37.0	39.0
11	38.36432025792585	39.0	39.0	39.0	37.0	39.0
12	38.33960236432026	39.0	39.0	39.0	37.0	39.0
13	38.327243417517465	39.0	39.0	39.0	37.0	39.0
14	39.842199534300555	41.0	40.0	41.0	38.0	41.0
15	39.865126276195596	41.0	40.0	41.0	38.0	41.0
16	39.783091527852406	41.0	40.0	41.0	38.0	41.0
17	39.810854379365935	41.0	40.0	41.0	38.0	41.0
18	39.79330109260254	41.0	40.0	41.0	38.0	41.0
19	39.78201683682608	41.0	40.0	41.0	38.0	41.0
20	39.81192907039226	41.0	40.0	41.0	38.0	41.0
21	39.71126634425936	41.0	40.0	41.0	38.0	41.0
22	39.69048898441698	41.0	40.0	41.0	38.0	41.0
23	39.680279419666846	41.0	40.0	41.0	37.0	41.0
24	39.636754433100485	41.0	40.0	41.0	37.0	41.0
25	39.63603797241626	41.0	40.0	41.0	37.0	41.0
26	39.478058391545765	41.0	39.0	41.0	37.0	41.0
27	39.35966326347842	41.0	39.0	41.0	36.0	41.0
28	39.3175711982805	41.0	39.0	41.0	36.0	41.0
29	39.26276195593767	41.0	39.0	41.0	36.0	41.0
30	39.182518359305035	41.0	39.0	41.0	35.0	41.0
31	39.15224789539674	41.0	39.0	41.0	35.0	41.0
32	39.115708400501525	41.0	39.0	41.0	35.0	41.0
33	39.022747626723984	40.0	39.0	41.0	35.0	41.0
34	39.00143292136844	40.0	39.0	41.0	35.0	41.0
35	38.9084721475909	40.0	39.0	41.0	35.0	41.0
36	38.80386888769479	40.0	38.0	41.0	35.0	41.0
37	38.76249328318109	40.0	38.0	41.0	35.0	41.0
38	38.69353394232491	40.0	38.0	41.0	35.0	41.0
39	38.545226580691384	40.0	38.0	41.0	35.0	41.0
40	38.529822675980654	40.0	38.0	41.0	35.0	41.0
41	38.461221565466595	40.0	37.0	41.0	35.0	41.0
42	38.381515314347126	40.0	37.0	41.0	35.0	41.0
43	37.641411427547915	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	0.0
23	2.0
24	3.0
25	5.0
26	8.0
27	20.0
28	20.0
29	17.0
30	32.0
31	44.0
32	52.0
33	61.0
34	113.0
35	151.0
36	230.0
37	493.0
38	1119.0
39	3212.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.113021672935695	16.406949668636933	12.645531076482177	27.83449758194519
2	21.97743148844707	19.72058033315422	31.09439369514598	27.207594483252734
3	21.40426294107111	19.451907576571735	29.07039226222461	30.073437220132544
4	15.493462296256494	15.188966505463014	33.97814794913129	35.339423249149206
5	18.216012896292316	31.900411964893426	31.25559734909547	18.627977789718788
6	33.97814794913129	33.40497940175533	14.830736163353036	17.78613648576034
7	29.643560809600572	27.046390829303242	19.416084542360736	23.893963818735447
8	27.565824825362707	30.395844528031525	19.21905785420025	22.819272792405517
9	26.347841662188788	12.341035285688697	17.427906143650368	43.88321690847215
10	19.738491850259717	22.317750313451548	28.89127709116962	29.052480745119112
11	38.151531434712524	20.651979222640158	18.233924413397816	22.962564929249506
12	23.55364499373097	23.679025613469463	25.70302704639083	27.06430234640874
13	35.91259179652517	17.23087945548988	20.777359842378647	26.079168905606302
14	24.968654845065377	19.55937667920473	22.228192727924057	33.243775747805834
15	30.521225147770014	23.929786852946442	19.32652695683324	26.222461042450295
16	26.401576213505283	23.356618305570482	21.09976715027763	29.142038330646606
17	25.68511552928533	23.499910442414475	21.655024180548093	29.159949847752102
18	26.867275658248253	20.347483431846676	23.1058570660935	29.679383843811568
19	29.751029912233566	22.711803689772523	21.81622783449758	25.720938563496325
20	30.109260254343546	19.21905785420025	23.481998925308975	27.18968296614723
21	27.85240909905069	21.314705355543616	21.5296435608096	29.303241984596095
22	27.6732939279957	23.58946802794197	19.9892530897367	28.747984954325634
23	28.28228550958266	20.884828945011645	22.15654665950206	28.676338885903636
24	28.35393157800466	20.992298047644635	22.353573347662547	28.300197026688164
25	28.44348916353215	21.332616872649112	22.62224610424503	27.60164785957371
26	27.762851513523195	23.840229267418948	21.655024180548093	26.74189503850976
27	27.565824825362707	22.729715206878023	23.087945548988	26.61651441877127
28	26.437399247716282	22.568511552928534	23.016299480566005	27.977789718789182
29	27.153859931936235	22.76553824108902	21.04603259896113	29.034569228013613
30	26.329930145083292	23.087945548988	23.840229267418948	26.74189503850976
31	28.300197026688164	21.010209564750134	21.601289629231594	29.08830377933011
32	26.455310764821782	22.532688518717535	22.693892172667024	28.318108543793656
33	24.968654845065377	21.493820526598604	25.595557943757836	27.941966684578183
34	25.39853125559735	21.85205086870858	24.288017195056423	28.46140068063765
35	26.652337452982266	21.457997492387605	25.953788285867812	25.935876768762313
36	26.580691384560275	21.655024180548093	24.46713236611141	27.29715206878023
37	25.52391187533584	22.084900591080064	23.481998925308975	28.909188608275123
38	24.43130933190041	19.52355364499373	26.580691384560275	29.464445638545584
39	24.14472505821243	19.236969371305747	27.941966684578183	28.676338885903636
40	23.786494716102453	20.490775568690665	29.303241984596095	26.41948773061078
41	24.359663263478417	18.001074691026332	28.998746193802617	28.640515851692637
42	21.314705355543616	19.290703922622246	31.166039763567976	28.228550958266162
43	20.86691742790614	19.86387246999821	30.969013075407485	28.300197026688164
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.5
7	2.0
8	2.0
9	2.0
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	5.5
16	9.0
17	8.5
18	8.0
19	8.0
20	9.0
21	10.0
22	10.0
23	10.0
24	9.0
25	8.0
26	8.0
27	16.0
28	24.0
29	23.5
30	23.0
31	35.5
32	48.0
33	48.0
34	56.0
35	64.0
36	77.0
37	90.0
38	101.0
39	112.0
40	112.0
41	131.0
42	150.0
43	171.5
44	193.0
45	274.0
46	355.0
47	355.0
48	423.5
49	492.0
50	525.0
51	558.0
52	568.0
53	578.0
54	578.0
55	593.5
56	609.0
57	610.0
58	611.0
59	525.5
60	440.0
61	440.0
62	392.0
63	344.0
64	306.0
65	268.0
66	236.0
67	204.0
68	204.0
69	169.0
70	134.0
71	120.0
72	106.0
73	76.5
74	47.0
75	47.0
76	36.5
77	26.0
78	26.0
79	26.0
80	19.0
81	12.0
82	12.0
83	11.5
84	11.0
85	6.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5583.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.44671323661113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.24484032152944	75.2283718430951
2	4.627416902020422	7.630306286942504
3	1.6945470345426896	4.1912950026867275
4	0.977623289159244	3.2240730789897905
5	0.49967412557028024	2.059824467132366
6	0.36932435368238103	1.8269747447608813
7	0.15207473386921572	0.877664338169443
8	0.08689984792526613	0.5731685473759627
9	0.021724961981316532	0.16120365394948952
>10	0.325874429719748	4.227118036897725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	50	0.8955758552749419	Illumina PCR Primer Index 10 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	18	0.32240730789897903	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	18	0.32240730789897903	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	16	0.28658427368798134	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	16	0.28658427368798134	Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	14	0.2507612394769837	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	14	0.2507612394769837	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	14	0.2507612394769837	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	13	0.23284972237148488	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	12	0.21493820526598603	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	11	0.1970266881604872	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	10	0.17911517105498836	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	10	0.17911517105498836	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	10	0.17911517105498836	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	10	0.17911517105498836	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	9	0.16120365394948952	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	8	0.14329213684399067	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	8	0.14329213684399067	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	8	0.14329213684399067	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	8	0.14329213684399067	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	7	0.12538061973849185	No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCG	7	0.12538061973849185	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	7	0.12538061973849185	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	7	0.12538061973849185	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	7	0.12538061973849185	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.12538061973849185	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	7	0.12538061973849185	No Hit
CAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCT	6	0.10746910263299302	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	6	0.10746910263299302	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACCTG	6	0.10746910263299302	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	6	0.10746910263299302	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	6	0.10746910263299302	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	6	0.10746910263299302	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	6	0.10746910263299302	No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT	6	0.10746910263299302	No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT	6	0.10746910263299302	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	6	0.10746910263299302	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	6	0.10746910263299302	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	6	0.10746910263299302	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	6	0.10746910263299302	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	6	0.10746910263299302	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	6	0.10746910263299302	No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA	6	0.10746910263299302	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	6	0.10746910263299302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.017911517105498837	0.0
24	0.0	0.0	0.0	0.017911517105498837	0.0
25	0.0	0.0	0.0	0.017911517105498837	0.0
26	0.0	0.0	0.0	0.017911517105498837	0.0
27	0.0	0.0	0.0	0.08955758552749418	0.0
28	0.0	0.0	0.0	0.12538061973849185	0.0
29	0.0	0.0	0.0	0.32240730789897903	0.0
30	0.0	0.0	0.0	0.5552570302704639	0.0
31	0.0	0.0	0.0	1.182160128962923	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE