FastQCFastQC Report
Fri 10 Feb 2017
ERR1632578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114588
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5720.49917966977344924No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4720.41191049673613295No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4570.39882012078053547No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4260.37176667713896744No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4150.36216706810486265No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3990.348204000418892No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3690.32202324850769715No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3630.3167870981254582No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3420.2984605717876217No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3070.2679163612245611No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2870.2504625266170978No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2830.24697175969560511No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2680.23388138374000766No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2640.23039061681851503No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT2520.21991831605403708TruSeq Adapter, Index 3 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2420.21119139875030546No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2310.20159178971620065No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2210.19286487241246902No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2180.19024679722134955No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2150.18762872203023004No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2130.18588333856948372No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2050.17890180472649841No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2030.1771564212657521No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1980.17279296261388627No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1930.16842950396202047No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1910.16668412050127412No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1810.1579572031975425No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1810.1579572031975425No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1790.15621181973679618No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1780.15533912800642302No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1760.15359374454567667No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1760.15359374454567667No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1710.14923028589381085No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1670.14573951897231824No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1620.1413760603204524No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1610.14050336859007923No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1600.13963067685970607No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1590.1387579851293329No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1590.1387579851293329No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1560.13613990993821343No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1470.12828568436485496No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1470.12828568436485496No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1450.12654030090410862No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1420.12392222571298915No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1390.12130415052186966No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1390.12130415052186966No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1370.11955876706112334No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1370.11955876706112334No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1330.11606800013963067No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1320.11519530840925751No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1310.11432261667888435No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1300.11344992494851118No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1300.11344992494851118No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG1270.1108318497573917No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1250.10908646629664537No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1240.10821377456627221No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1230.10734108283589905No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1220.10646839110552589No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1220.10646839110552589No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT1160.10123224072328692No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1160.10123224072328692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCC200.001835027637.035
TATGCCC200.001835027637.036
GTGATTC451.0655822E-732.8888916
GATTCCT451.0655822E-732.8888918
GATATGC352.3697217E-531.71428734
TAAACCA250.005475963529.65
ACGGGAC250.005475963529.615
GGTGATT502.6847738E-729.615
TGGGCAG502.6847738E-729.66
ACTCCCA250.005475963529.65
AGTCGGT502.6847738E-729.611
ATTGATA453.966994E-628.77777731
GGTATCA2650.028.6226431
TTGATAT405.8942755E-527.7532
CCTTATT405.8942755E-527.7527
GCAGTCG556.179416E-726.909099
CTTATTG556.179416E-726.9090928
CAGTCGG556.179416E-726.9090910
TTATTGA556.179416E-726.9090929
GTCGGTG556.179416E-726.9090912
Produced by FastQC (version 0.11.5)