##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632578.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	114588
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07565364610605	34.0	33.0	34.0	31.0	34.0
2	33.20155688204699	34.0	33.0	34.0	31.0	34.0
3	33.276974901385834	34.0	34.0	34.0	31.0	34.0
4	36.543433867420674	37.0	37.0	37.0	35.0	37.0
5	36.51528955911614	37.0	37.0	37.0	35.0	37.0
6	36.575042761894785	37.0	37.0	37.0	35.0	37.0
7	36.583726044612	37.0	37.0	37.0	35.0	37.0
8	36.552466226830035	37.0	37.0	37.0	35.0	37.0
9	38.35093552553496	39.0	39.0	39.0	37.0	39.0
10	38.38626208678046	39.0	39.0	39.0	37.0	39.0
11	38.45600760987189	39.0	39.0	39.0	37.0	39.0
12	38.40875135267218	39.0	39.0	39.0	37.0	39.0
13	38.449785317834326	39.0	39.0	39.0	37.0	39.0
14	39.977510734108286	41.0	40.0	41.0	38.0	41.0
15	39.99998254616539	41.0	40.0	41.0	38.0	41.0
16	39.93432122037211	41.0	40.0	41.0	38.0	41.0
17	39.952307396935105	41.0	40.0	41.0	38.0	41.0
18	39.966287918455684	41.0	40.0	41.0	38.0	41.0
19	39.95400914580934	41.0	40.0	41.0	38.0	41.0
20	39.951600516633505	41.0	40.0	41.0	38.0	41.0
21	39.90612455056376	41.0	40.0	41.0	38.0	41.0
22	39.88518867595211	41.0	40.0	41.0	38.0	41.0
23	39.84829126959193	41.0	40.0	41.0	38.0	41.0
24	39.81776451286348	41.0	40.0	41.0	38.0	41.0
25	39.778545746500505	41.0	40.0	41.0	38.0	41.0
26	39.69226271511851	41.0	40.0	41.0	37.0	41.0
27	39.607986874716374	41.0	40.0	41.0	37.0	41.0
28	39.56795650504416	41.0	40.0	41.0	37.0	41.0
29	39.53353754319824	41.0	40.0	41.0	37.0	41.0
30	39.49644814465738	41.0	40.0	41.0	37.0	41.0
31	39.41177086605927	41.0	39.0	41.0	36.0	41.0
32	39.37234265368102	41.0	39.0	41.0	36.0	41.0
33	39.28790972876741	41.0	39.0	41.0	36.0	41.0
34	39.26805599190142	41.0	39.0	41.0	35.0	41.0
35	39.19039515481551	41.0	39.0	41.0	35.0	41.0
36	39.13876671204664	41.0	39.0	41.0	35.0	41.0
37	39.07760847558208	41.0	39.0	41.0	35.0	41.0
38	39.00016581142877	41.0	39.0	41.0	35.0	41.0
39	38.89642021852201	41.0	39.0	41.0	35.0	41.0
40	38.81141131706636	40.0	38.0	41.0	35.0	41.0
41	38.76713966558453	40.0	38.0	41.0	35.0	41.0
42	38.70185359723531	40.0	38.0	41.0	35.0	41.0
43	37.98696198554823	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	7.0
21	12.0
22	20.0
23	41.0
24	57.0
25	72.0
26	126.0
27	170.0
28	274.0
29	363.0
30	442.0
31	630.0
32	816.0
33	1156.0
34	1734.0
35	2776.0
36	4319.0
37	8293.0
38	21501.0
39	71775.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.65095821551995	16.299263448179566	13.868816979090306	28.180961357210176
2	21.236080566900547	19.73243271546759	31.492826473976333	27.53866024365553
3	21.18633713826928	19.093622368834435	30.137536216706813	29.582504276189482
4	15.835864139351417	17.220825915453624	34.42507068803016	32.5182392571648
5	17.151010577023772	31.765978985583132	33.67717387510036	17.405836562292738
6	31.310433902328345	34.024505183788875	16.68150242608301	17.98355848779977
7	28.57105456068698	27.435682619471496	20.586797919502914	23.406464900338605
8	26.775054979579018	29.4908716445003	21.938597409850942	21.79547596606975
9	28.684504485635493	12.27528187942891	18.177296051942612	40.862917582992985
10	20.213285858903202	22.47530282403044	28.315355883687648	28.996055433378714
11	39.22836597200405	18.882430970084126	18.26194714978881	23.627255908123015
12	21.858309770656614	24.250357803609454	26.18075191119489	27.710580514539046
13	36.51080392362202	17.310713163682063	20.29880964847977	25.879673264216148
14	23.73634237441966	21.83300171047579	23.310468809997555	31.120187105106993
15	31.04949907494677	23.731106224037422	19.64516354243027	25.57423115858554
16	24.137780570391314	24.230285893810873	23.24239885502845	28.389534680769362
17	27.179983942472163	24.248612420148707	20.9384926868433	27.632910950535834
18	26.81170803225469	20.33459000942507	22.622787726463503	30.230914231856737
19	28.290047823506825	22.07211924459804	23.052152057807103	26.585680874088034
20	31.332251195587666	19.464516354243028	22.769399937166195	26.433832513003107
21	27.564840995566726	21.938597409850942	21.6994798757287	28.797081718853633
22	29.61566656194366	22.49188396690753	20.710720145215905	27.181729325932906
23	28.847697839215275	21.536286522148917	21.709079484762803	27.90693615387301
24	28.37644430481377	20.87827695744755	23.127203546619192	27.61807519111949
25	28.36073585366705	21.80158480818236	23.012008238209937	26.825671099940656
26	28.261248996404507	23.41082835899047	21.912416657939747	26.415505986665273
27	26.413760603204523	21.773658672810416	25.501797744964566	26.31078297902049
28	25.864837504799805	22.96313750130904	21.609592627500263	29.562432366390894
29	27.591894439208293	23.5626767200754	21.877508988724824	26.967919851991486
30	26.952211400844767	21.235207875170175	23.85153768282892	27.96104304115614
31	28.54749188396691	22.015394282123783	22.5643173805285	26.872796453380808
32	26.19471497888086	21.564212657520855	22.08870038747513	30.152371976123156
33	25.689426466994796	21.218626732293085	24.99127308269627	28.10067371801585
34	26.068174677976753	21.345167033197193	24.04003909658952	28.546619192236534
35	25.387475128285686	24.01298565294795	24.972073864628058	25.627465354138305
36	26.388452543023703	22.246657590672672	24.924948511187907	26.43994135511572
37	26.94697525046253	22.423814011938422	24.239012811114602	26.390197926484447
38	26.37012601668587	21.110412957726812	25.239990225852623	27.2794707997347
39	26.058575068942645	19.869445317136176	26.60662547561699	27.46535413830419
40	25.081160330924707	21.68377142458198	27.511606800013965	25.72346144447935
41	22.908157922295526	20.354661919223656	28.23681362795406	28.500366530526755
42	20.966418822215243	21.140957168289873	29.946416727755086	27.9462072817398
43	20.32848116731246	22.457848989422978	29.073724997381923	28.139944845882642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	6.0
4	9.0
5	9.0
6	9.5
7	10.0
8	10.0
9	10.0
10	18.5
11	27.0
12	27.0
13	46.0
14	65.0
15	121.0
16	177.0
17	161.0
18	145.0
19	145.0
20	176.0
21	207.0
22	175.0
23	143.0
24	127.5
25	112.0
26	112.0
27	124.5
28	137.0
29	224.5
30	312.0
31	345.5
32	379.0
33	379.0
34	517.5
35	656.0
36	676.5
37	697.0
38	1097.0
39	1497.0
40	1497.0
41	1909.0
42	2321.0
43	3021.5
44	3722.0
45	6822.5
46	9923.0
47	9923.0
48	11090.5
49	12258.0
50	14203.5
51	16149.0
52	16072.5
53	15996.0
54	15996.0
55	14257.5
56	12519.0
57	11776.5
58	11034.0
59	9779.5
60	8525.0
61	8525.0
62	7533.5
63	6542.0
64	5102.5
65	3663.0
66	3123.5
67	2584.0
68	2584.0
69	2129.0
70	1674.0
71	1407.5
72	1141.0
73	861.5
74	582.0
75	582.0
76	485.0
77	388.0
78	354.5
79	321.0
80	299.0
81	277.0
82	277.0
83	237.5
84	198.0
85	135.5
86	73.0
87	71.0
88	69.0
89	69.0
90	47.0
91	25.0
92	20.0
93	15.0
94	8.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	114588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.97657695395679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47557162003005	34.585645966418824
2	8.40677415424603	7.22588752748979
3	3.2205661373512573	4.152267253115509
4	1.7199366446005768	2.956679582504276
5	1.100597002802258	2.3649945893112716
6	0.7350850830524306	1.8954864383705101
7	0.5970028022580515	1.7959995811079696
8	0.4386143036997929	1.5080113100848256
9	0.3330219713276205	1.2880929940307886
>10	2.558583438248792	21.592138792892797
>50	0.25382772204849124	7.660488009215624
>100	0.15838849855825854	12.475128285684365
>500	0.0020306217763879303	0.49917966977344924
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	572	0.49917966977344924	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	472	0.41191049673613295	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	457	0.39882012078053547	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	426	0.37176667713896744	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	415	0.36216706810486265	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	399	0.348204000418892	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	369	0.32202324850769715	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	363	0.3167870981254582	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	342	0.2984605717876217	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	307	0.2679163612245611	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	287	0.2504625266170978	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	283	0.24697175969560511	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	268	0.23388138374000766	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	264	0.23039061681851503	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	252	0.21991831605403708	TruSeq Adapter, Index 3 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	242	0.21119139875030546	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	231	0.20159178971620065	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	221	0.19286487241246902	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	218	0.19024679722134955	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	215	0.18762872203023004	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	213	0.18588333856948372	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	205	0.17890180472649841	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	203	0.1771564212657521	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	198	0.17279296261388627	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	193	0.16842950396202047	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	191	0.16668412050127412	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	181	0.1579572031975425	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	181	0.1579572031975425	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	179	0.15621181973679618	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	178	0.15533912800642302	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	176	0.15359374454567667	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	176	0.15359374454567667	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	171	0.14923028589381085	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	167	0.14573951897231824	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	162	0.1413760603204524	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	161	0.14050336859007923	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	160	0.13963067685970607	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	159	0.1387579851293329	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	159	0.1387579851293329	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	156	0.13613990993821343	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	147	0.12828568436485496	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	147	0.12828568436485496	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	145	0.12654030090410862	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	142	0.12392222571298915	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	139	0.12130415052186966	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	139	0.12130415052186966	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	137	0.11955876706112334	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	137	0.11955876706112334	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	133	0.11606800013963067	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	132	0.11519530840925751	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	131	0.11432261667888435	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	130	0.11344992494851118	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	130	0.11344992494851118	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG	127	0.1108318497573917	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	125	0.10908646629664537	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	124	0.10821377456627221	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	123	0.10734108283589905	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	122	0.10646839110552589	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	122	0.10646839110552589	No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT	116	0.10123224072328692	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	116	0.10123224072328692	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.72691730373163E-4	0.0
4	0.0	0.0	0.0	8.72691730373163E-4	0.0
5	0.0	0.0	0.0	8.72691730373163E-4	0.0
6	0.0	0.0	0.0	8.72691730373163E-4	0.0
7	0.0	0.0	0.0	8.72691730373163E-4	0.0
8	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
9	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
10	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
11	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
12	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
13	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
14	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
15	8.72691730373163E-4	0.0	0.0	8.72691730373163E-4	0.0
16	8.72691730373163E-4	0.0	0.0	0.001745383460746326	0.0
17	8.72691730373163E-4	0.0	0.0	0.001745383460746326	0.0
18	8.72691730373163E-4	0.0	0.0	0.002618075191119489	0.0
19	8.72691730373163E-4	0.0	0.0	0.0043634586518658145	0.0
20	8.72691730373163E-4	0.0	0.0	0.006108842112612141	0.0
21	0.001745383460746326	0.0	0.0	0.013090375955597445	0.0
22	0.001745383460746326	0.0	0.0	0.015708451146716932	0.0
23	0.001745383460746326	0.0	0.0	0.020071909798582747	0.0
24	0.001745383460746326	0.0	0.0	0.025308060180821726	0.0
25	0.001745383460746326	0.0	0.0	0.025308060180821726	0.0
26	0.001745383460746326	0.0	0.0	0.03228959402380703	0.0
27	0.001745383460746326	0.0	0.0	0.05497957901350927	0.0
28	0.001745383460746326	0.0	0.0	0.14835759416343772	0.0
29	0.001745383460746326	0.0	0.0	0.3045694139002339	0.0
30	0.001745383460746326	0.0	0.0	0.5489230984047195	0.0
31	0.001745383460746326	0.0	0.0	1.1266450239117534	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCC	20	0.0018350276	37.0	35
TATGCCC	20	0.0018350276	37.0	36
GTGATTC	45	1.0655822E-7	32.88889	16
GATTCCT	45	1.0655822E-7	32.88889	18
GATATGC	35	2.3697217E-5	31.714287	34
TAAACCA	25	0.0054759635	29.6	5
ACGGGAC	25	0.0054759635	29.6	15
GGTGATT	50	2.6847738E-7	29.6	15
TGGGCAG	50	2.6847738E-7	29.6	6
ACTCCCA	25	0.0054759635	29.6	5
AGTCGGT	50	2.6847738E-7	29.6	11
ATTGATA	45	3.966994E-6	28.777777	31
GGTATCA	265	0.0	28.622643	1
TTGATAT	40	5.8942755E-5	27.75	32
CCTTATT	40	5.8942755E-5	27.75	27
GCAGTCG	55	6.179416E-7	26.90909	9
CTTATTG	55	6.179416E-7	26.90909	28
CAGTCGG	55	6.179416E-7	26.90909	10
TTATTGA	55	6.179416E-7	26.90909	29
GTCGGTG	55	6.179416E-7	26.90909	12
>>END_MODULE