FastQCFastQC Report
Fri 10 Feb 2017
ERR1632577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26588
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT950.35730404693846846RNA PCR Primer, Index 41 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA920.34602076124567477No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA680.2557544757033248No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA650.24447119001053105No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA610.22942680908680607No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT600.22566571385587483No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA580.21814352339401233No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC560.21062133293214985No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG530.19933804723935608No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA460.17301038062283738No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT450.16924928539190612No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC440.16548819016097488No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA410.15420490446818114No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA380.1429216187753874No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA380.1429216187753874No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT370.13916052354445613No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC350.13163833308259365No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC340.1278772378516624No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT330.12411614262073116No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC320.12035504738979991No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT320.12035504738979991No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA320.12035504738979991No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA310.11659395215886867No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC290.10907176169700616No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG290.10907176169700616No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT280.10531066646607493No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA280.10531066646607493No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA280.10531066646607493No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC280.10531066646607493No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC280.10531066646607493No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC270.10154957123514367No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC270.10154957123514367No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA270.10154957123514367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352.302256E-531.7142871
CTTATAC250.00540137229.637
ACGGTGG250.00540137229.67
TCTTATA250.00540137229.636
TCCCTTC250.00540137229.622
TGACCAT451.2762878E-424.6666668
AGTGACC451.2762878E-424.6666666
TTAGTGA451.2762878E-424.6666664
TAGTGAC502.6070417E-422.25
AACGACA608.917044E-418.537
TAACGAC608.917044E-418.536
CTCTTAT500.006859826418.537
GTATCAA701.1632944E-418.51
GTGACCA701.1632944E-418.57
GACCATG608.917044E-418.59
GCCTCGG751.9731387E-417.26666626
GAAGTGC650.001525678417.07692315
TGAAGTG650.001525678417.07692314
CTCGGTG650.001525678417.07692328
TCTCTTA904.188921E-516.44444537
Produced by FastQC (version 0.11.5)