##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26588
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.018278922822326	34.0	33.0	34.0	31.0	34.0
2	33.168271400631866	34.0	33.0	34.0	31.0	34.0
3	33.2362720024071	34.0	34.0	34.0	31.0	34.0
4	36.52798254851813	37.0	37.0	37.0	35.0	37.0
5	36.498081841432224	37.0	37.0	37.0	35.0	37.0
6	36.53283436136603	37.0	37.0	37.0	35.0	37.0
7	36.52572589137957	37.0	37.0	37.0	35.0	37.0
8	36.51004212426658	37.0	37.0	37.0	35.0	37.0
9	38.302993831803825	39.0	39.0	39.0	37.0	39.0
10	38.31506694749511	39.0	39.0	39.0	37.0	39.0
11	38.38923574544908	39.0	39.0	39.0	37.0	39.0
12	38.34327516172709	39.0	39.0	39.0	37.0	39.0
13	38.39495261020009	39.0	39.0	39.0	37.0	39.0
14	39.950428764856326	41.0	40.0	41.0	38.0	41.0
15	39.92827591394614	41.0	40.0	41.0	38.0	41.0
16	39.8881450278321	41.0	40.0	41.0	38.0	41.0
17	39.91285542349932	41.0	40.0	41.0	38.0	41.0
18	39.89882653828795	41.0	40.0	41.0	38.0	41.0
19	39.91368286445013	41.0	40.0	41.0	38.0	41.0
20	39.89318489544155	41.0	40.0	41.0	38.0	41.0
21	39.870279825485184	41.0	40.0	41.0	38.0	41.0
22	39.80852264179329	41.0	40.0	41.0	38.0	41.0
23	39.78840078230781	41.0	40.0	41.0	38.0	41.0
24	39.746126071912144	41.0	40.0	41.0	38.0	41.0
25	39.7019332029487	41.0	40.0	41.0	38.0	41.0
26	39.65194824732962	41.0	40.0	41.0	37.0	41.0
27	39.53159319993982	41.0	40.0	41.0	37.0	41.0
28	39.500752219046184	41.0	40.0	41.0	37.0	41.0
29	39.464231984353844	41.0	40.0	41.0	37.0	41.0
30	39.42549270347525	41.0	39.0	41.0	36.0	41.0
31	39.343312772679404	41.0	39.0	41.0	36.0	41.0
32	39.28723484278622	41.0	39.0	41.0	36.0	41.0
33	39.18602377012186	41.0	39.0	41.0	35.0	41.0
34	39.150782307808036	41.0	39.0	41.0	35.0	41.0
35	39.068038212727544	41.0	39.0	41.0	35.0	41.0
36	39.029712652324356	41.0	39.0	41.0	35.0	41.0
37	38.968895742440196	41.0	39.0	41.0	35.0	41.0
38	38.897510154957125	40.0	39.0	41.0	35.0	41.0
39	38.77978787422897	40.0	38.0	41.0	35.0	41.0
40	38.6974951105762	40.0	38.0	41.0	35.0	41.0
41	38.64386189258312	40.0	38.0	41.0	35.0	41.0
42	38.55698059274861	40.0	38.0	41.0	35.0	41.0
43	37.84041672935159	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	3.0
22	6.0
23	4.0
24	15.0
25	22.0
26	34.0
27	50.0
28	67.0
29	110.0
30	114.0
31	167.0
32	226.0
33	288.0
34	419.0
35	683.0
36	1069.0
37	2000.0
38	5122.0
39	16184.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.447720776290055	16.432225063938617	12.787723785166241	28.332330374605085
2	21.460809387693697	19.33202948698661	30.946291560102303	28.26086956521739
3	21.359259816458554	19.016097487588386	28.95291108770874	30.67173160824432
4	16.007221302843387	15.6235895892884	32.58236798555739	35.78682112231082
5	17.376259966902364	32.221302843387996	33.180382127275465	17.22205506243418
6	34.95938017150594	32.60117346171205	15.450579208665562	16.988867158116445
7	29.814201895591996	27.2867459004062	20.02030991424703	22.87874228975478
8	27.760643899503535	30.032345418986004	20.44907477057319	21.757935910937263
9	28.58056265984655	12.505641642846395	17.312321347976532	41.60147434933052
10	19.523845343764105	23.533172859936812	28.704678802467278	28.238302993831805
11	40.02557544757033	18.888220249736722	17.61320896645103	23.472995336241915
12	23.13825786068903	23.653527907326612	25.7221302843388	27.486083947645557
13	35.903415074469684	17.10170001504438	20.076726342710998	26.918158567774935
14	25.195576952008423	19.644200391153905	22.370994433579057	32.78922822325862
15	30.32194975176771	23.867910335489697	19.29817962990823	26.511960282834362
16	26.65112080637882	23.405295622085152	21.645103054009326	28.298480517526702
17	27.745599518579812	23.25109071761697	21.423198435384382	27.580111328418838
18	26.752670377613963	20.926733864901458	22.532721528509104	29.787874228975475
19	28.069053708439895	22.122762148337596	22.25063938618926	27.55754475703325
20	30.37084398976982	20.422747103956674	21.874529863096136	27.33187904317737
21	27.911087708740784	21.340454340303896	21.61877538739281	29.129682563562508
22	28.975477659094327	22.690687528208215	20.163231533022415	28.170603279675042
23	28.794945088009626	21.490898149541145	21.536031292312323	28.178125470136905
24	28.595607040770272	21.329171054611106	21.863246577403338	28.21197532721528
25	28.82503384985708	21.43072062584625	22.145328719723185	27.59891680457349
26	29.073266135098542	22.160373100646908	21.53979238754325	27.226568376711295
27	27.061080186550324	21.825635625094026	23.724988716714307	27.388295471641342
28	26.534526854219948	22.34842786219347	21.878290958327064	29.238754325259514
29	28.13299232736573	22.701970813901006	21.50218143523394	27.662855423499323
30	27.369489995486685	21.235143673837822	22.88626448021664	28.50910185045885
31	28.460207612456745	21.900857529712653	21.882052053557995	27.756882804272603
32	26.835414472694445	21.56612005415977	21.791785768015647	29.806679705130136
33	26.297577854671278	21.46457048292463	24.052204001805325	28.185647660598768
34	26.906875282082144	21.848202196479615	22.690687528208215	28.554234993230025
35	25.834963141266737	23.45795095531819	24.4584022867459	26.248683616669172
36	27.05355799608846	21.73913043478261	23.879193621182488	27.32811794794644
37	27.211523995787573	22.081390100797353	23.64224462163382	27.064841281781256
38	27.03851361516474	21.212577102452236	24.0973371445765	27.65157213780653
39	26.286294568978484	20.253497818564767	25.17301038062284	28.28719723183391
40	24.928539190612305	21.30284338799458	26.69249285391906	27.076124567474047
41	24.417030239205655	20.392658342109222	26.47058823529412	28.719723183391004
42	22.600421242665863	20.59575748457951	28.877689183090116	27.926132089664513
43	22.216789529110876	21.235143673837822	27.55754475703325	28.990522040018053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	3.0
9	3.0
10	3.0
11	3.0
12	3.0
13	9.0
14	15.0
15	16.5
16	18.0
17	19.0
18	20.0
19	20.0
20	23.5
21	27.0
22	23.0
23	19.0
24	26.0
25	33.0
26	33.0
27	44.0
28	55.0
29	74.0
30	93.0
31	115.0
32	137.0
33	137.0
34	184.5
35	232.0
36	269.5
37	307.0
38	400.5
39	494.0
40	494.0
41	592.0
42	690.0
43	829.5
44	969.0
45	1410.0
46	1851.0
47	1851.0
48	2030.0
49	2209.0
50	2560.5
51	2912.0
52	3058.5
53	3205.0
54	3205.0
55	2966.5
56	2728.0
57	2633.0
58	2538.0
59	2316.5
60	2095.0
61	2095.0
62	1968.5
63	1842.0
64	1572.5
65	1303.0
66	1116.0
67	929.0
68	929.0
69	793.0
70	657.0
71	579.0
72	501.0
73	387.0
74	273.0
75	273.0
76	229.0
77	185.0
78	144.5
79	104.0
80	85.5
81	67.0
82	67.0
83	51.5
84	36.0
85	27.5
86	19.0
87	13.5
88	8.0
89	8.0
90	5.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26588.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.14156762449225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.17058547520983	63.60764254550926
2	6.209269589698139	8.958928840078231
3	2.116677962567124	4.581013991274259
4	1.0322715186903708	2.9787874228975477
5	0.6099786246806735	2.20024071009478
6	0.3649444763046765	1.579659996991124
7	0.26588811845055005	1.3427109974424554
8	0.2763151034878265	1.5947043779148486
9	0.13033731296595588	0.8462464269595306
>10	0.7768103852770971	10.023318790431773
>50	0.04692143266774412	2.2867459004061983
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	95	0.35730404693846846	RNA PCR Primer, Index 41 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	92	0.34602076124567477	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	68	0.2557544757033248	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	65	0.24447119001053105	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	61	0.22942680908680607	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	60	0.22566571385587483	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	58	0.21814352339401233	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	56	0.21062133293214985	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	53	0.19933804723935608	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	46	0.17301038062283738	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	45	0.16924928539190612	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	44	0.16548819016097488	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	41	0.15420490446818114	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	38	0.1429216187753874	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	38	0.1429216187753874	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	37	0.13916052354445613	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	35	0.13163833308259365	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	34	0.1278772378516624	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	33	0.12411614262073116	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	32	0.12035504738979991	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	32	0.12035504738979991	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	32	0.12035504738979991	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	31	0.11659395215886867	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	29	0.10907176169700616	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	29	0.10907176169700616	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	28	0.10531066646607493	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	28	0.10531066646607493	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	28	0.10531066646607493	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	28	0.10531066646607493	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	28	0.10531066646607493	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	27	0.10154957123514367	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	27	0.10154957123514367	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	27	0.10154957123514367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.003761095230931247	0.0	0.0	0.0	0.0
6	0.003761095230931247	0.0	0.0	0.0	0.0
7	0.003761095230931247	0.0	0.0	0.0	0.0
8	0.003761095230931247	0.0	0.0	0.0	0.0
9	0.003761095230931247	0.0	0.0	0.0	0.0
10	0.003761095230931247	0.0	0.0	0.0	0.0
11	0.003761095230931247	0.0	0.0	0.0	0.0
12	0.003761095230931247	0.0	0.0	0.0	0.0
13	0.003761095230931247	0.0	0.0	0.0	0.0
14	0.003761095230931247	0.0	0.0	0.0	0.0
15	0.003761095230931247	0.0	0.0	0.0	0.0
16	0.003761095230931247	0.0	0.0	0.0	0.0
17	0.003761095230931247	0.0	0.0	0.0	0.0
18	0.003761095230931247	0.0	0.0	0.0	0.0
19	0.003761095230931247	0.0	0.0	0.0	0.0
20	0.003761095230931247	0.0	0.0	0.0	0.0
21	0.003761095230931247	0.0	0.0	0.007522190461862494	0.0
22	0.003761095230931247	0.0	0.0	0.011283285692793741	0.0
23	0.003761095230931247	0.0	0.0	0.011283285692793741	0.0
24	0.003761095230931247	0.0	0.0	0.018805476154656237	0.0
25	0.003761095230931247	0.0	0.0	0.018805476154656237	0.0
26	0.003761095230931247	0.0	0.0	0.018805476154656237	0.0
27	0.003761095230931247	0.0	0.0	0.03384985707838122	0.0
28	0.003761095230931247	0.0	0.0	0.08650519031141868	0.0
29	0.003761095230931247	0.0	0.0	0.15796599969911237	0.0
30	0.003761095230931247	0.0	0.0	0.31593199939822475	0.0
31	0.003761095230931247	0.0	0.0	0.6807582367985557	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	2.302256E-5	31.714287	1
CTTATAC	25	0.005401372	29.6	37
ACGGTGG	25	0.005401372	29.6	7
TCTTATA	25	0.005401372	29.6	36
TCCCTTC	25	0.005401372	29.6	22
TGACCAT	45	1.2762878E-4	24.666666	8
AGTGACC	45	1.2762878E-4	24.666666	6
TTAGTGA	45	1.2762878E-4	24.666666	4
TAGTGAC	50	2.6070417E-4	22.2	5
AACGACA	60	8.917044E-4	18.5	37
TAACGAC	60	8.917044E-4	18.5	36
CTCTTAT	50	0.0068598264	18.5	37
GTATCAA	70	1.1632944E-4	18.5	1
GTGACCA	70	1.1632944E-4	18.5	7
GACCATG	60	8.917044E-4	18.5	9
GCCTCGG	75	1.9731387E-4	17.266666	26
GAAGTGC	65	0.0015256784	17.076923	15
TGAAGTG	65	0.0015256784	17.076923	14
CTCGGTG	65	0.0015256784	17.076923	28
TCTCTTA	90	4.188921E-5	16.444445	37
>>END_MODULE