FastQCFastQC Report
Fri 10 Feb 2017
ERR1632576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804715
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA27820.15415176357485808No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA22290.12350980625749772No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA21190.11741466104066293No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19560.10838276403753501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.027.4916211
TAGTACG450.003826641220.5555554
ACTATAT851.2461132E-619.5882363
CGTTATT1052.2615495E-819.3809510
ATTCGCG609.239597E-418.530
CTTATAC22100.018.33257937
GTATCAA26550.018.32582
TATACAC6750.017.81481437
TAATACG12800.017.77734427
ATACGGC2750.017.49090829
GCGGTAA14100.017.31914923
GGTAATA14000.017.31071525
GGACGTA2150.017.20930327
TAGCGAA2050.017.14634110
ATCTAAG4900.016.98979832
CGGTAAT14350.016.75958324
AACTCCG14700.016.7380945
GTAACGT7850.016.49681526
GTCTAGT904.4482178E-516.4444451
AACGTCA7800.016.36538528
Produced by FastQC (version 0.11.5)