##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1804715
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.083775554588954	34.0	33.0	34.0	31.0	34.0
2	33.2201577534403	34.0	33.0	34.0	31.0	34.0
3	33.29507595382096	34.0	34.0	34.0	31.0	34.0
4	36.566996450963174	37.0	37.0	37.0	35.0	37.0
5	36.5350235355721	37.0	37.0	37.0	35.0	37.0
6	36.57664949867431	37.0	37.0	37.0	35.0	37.0
7	36.572841141122005	37.0	37.0	37.0	35.0	37.0
8	36.5496308281363	37.0	37.0	37.0	35.0	37.0
9	38.34512984044572	39.0	39.0	39.0	37.0	39.0
10	38.37793834483561	39.0	39.0	39.0	37.0	39.0
11	38.44581221965795	39.0	39.0	39.0	37.0	39.0
12	38.40808050024519	39.0	39.0	39.0	37.0	39.0
13	38.441514034071865	39.0	39.0	39.0	37.0	39.0
14	40.014673785057475	41.0	40.0	41.0	38.0	41.0
15	40.003052005441305	41.0	40.0	41.0	38.0	41.0
16	39.9793568513588	41.0	40.0	41.0	38.0	41.0
17	39.972879928409746	41.0	40.0	41.0	38.0	41.0
18	39.96203721917311	41.0	40.0	41.0	38.0	41.0
19	39.965187855146105	41.0	40.0	41.0	38.0	41.0
20	39.92996955197912	41.0	40.0	41.0	38.0	41.0
21	39.90488304247485	41.0	40.0	41.0	38.0	41.0
22	39.87634668077785	41.0	40.0	41.0	38.0	41.0
23	39.8380209617585	41.0	40.0	41.0	38.0	41.0
24	39.82210487528502	41.0	40.0	41.0	38.0	41.0
25	39.77652039241653	41.0	40.0	41.0	38.0	41.0
26	39.70137002241351	41.0	40.0	41.0	38.0	41.0
27	39.63150635972993	41.0	40.0	41.0	37.0	41.0
28	39.58708217086908	41.0	40.0	41.0	37.0	41.0
29	39.54329963456834	41.0	40.0	41.0	37.0	41.0
30	39.49742757166644	41.0	40.0	41.0	37.0	41.0
31	39.43531028444934	41.0	39.0	41.0	36.0	41.0
32	39.38219829723807	41.0	39.0	41.0	36.0	41.0
33	39.31391216895742	41.0	39.0	41.0	36.0	41.0
34	39.2690768348465	41.0	39.0	41.0	35.0	41.0
35	39.197979182308565	41.0	39.0	41.0	35.0	41.0
36	39.14974774410364	41.0	39.0	41.0	35.0	41.0
37	39.091334642866045	41.0	39.0	41.0	35.0	41.0
38	39.01246013913554	41.0	39.0	41.0	35.0	41.0
39	38.930285945426284	41.0	39.0	41.0	35.0	41.0
40	38.85447508332341	40.0	38.0	41.0	35.0	41.0
41	38.79917992591628	40.0	38.0	41.0	35.0	41.0
42	38.72856822268336	40.0	38.0	41.0	35.0	41.0
43	38.001184120484396	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	4.0
17	13.0
18	27.0
19	45.0
20	85.0
21	181.0
22	336.0
23	583.0
24	917.0
25	1451.0
26	1926.0
27	2800.0
28	3932.0
29	5340.0
30	7366.0
31	9702.0
32	12861.0
33	17316.0
34	26079.0
35	42746.0
36	69686.0
37	132715.0
38	337216.0
39	1131381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.944722573924416	15.776175185555614	11.921771581662478	28.357330658857492
2	21.43790016706239	18.37774939533389	31.74479072873002	28.439559708873702
3	21.765818979728103	18.940940813369426	27.785772268751575	31.507467938150903
4	16.333326868785374	14.738282775950772	31.999179925916284	36.92921042934757
5	18.2305239331418	32.0070482042871	32.21012736082983	17.55230050174127
6	37.0099433982651	31.81172650529308	14.373626860750866	16.804703235690955
7	32.02483494623805	26.555439501527943	18.928861343757877	22.49086420847613
8	28.696885657846256	30.268435736390508	18.894562299310415	22.14011630645282
9	28.412020734575822	12.658065123856122	16.955308732957835	41.97460540861022
10	19.688150206542307	22.973156426360948	29.429577523320855	27.90911584377589
11	40.56136287447048	18.710211861706696	18.255015334831263	22.473409928991558
12	23.969158565202818	22.514856916466037	26.07209448583294	27.44389003249821
13	35.37832843412949	17.093391477324673	20.01795297318413	27.510327115361704
14	24.789454290566653	18.599834322870922	22.229105426618606	34.38160595994381
15	29.639305929191035	23.422756501719107	19.335241298487574	27.602696270602284
16	27.230227487442615	22.869095674386262	21.421166222921624	28.4795106152495
17	27.587070534682763	22.725416478502144	21.50250870636084	28.185004280454258
18	27.5772074815137	21.12821137963612	22.604344730331384	28.6902364085188
19	28.879241320651737	21.72686546075142	22.19724444025788	27.19664877833896
20	29.830804309821772	20.809546105617784	21.946290688557475	27.413358896002972
21	28.376779713140305	21.568890378813276	21.33045938001291	28.723870528033512
22	29.05710874016119	22.081547501960145	20.767046320333126	28.09429743754554
23	28.768919192227028	21.615656765749716	21.52871783079323	28.086706211230027
24	28.668127654505003	21.270893188121114	21.49585945703338	28.565119700340496
25	28.61637433057297	21.424102974707917	21.967568286405335	27.99195440831378
26	28.439227246407327	21.88561628844444	21.693452982880952	27.981703482267285
27	27.96297476332828	21.441501843781428	22.614374014733627	27.981149378156662
28	27.465001399112882	21.95609833131547	21.687025375197745	28.891874894373903
29	28.0206015908329	22.484159548737612	21.657103753224195	27.838135107205293
30	27.80533214385651	21.59919987366426	22.547161186115257	28.04830679636397
31	28.15469478560327	21.61449314711741	21.963467915986733	28.267344151292583
32	26.645426009092848	21.66142576528704	22.295985792770605	29.397162432849512
33	26.997780813036957	21.31150901942966	23.32567746153825	28.36503270599513
34	27.16545271691098	20.98021017168916	23.272705108562846	28.581632002837015
35	26.668864612972133	21.89902560792147	23.767575489758773	27.664534289347625
36	26.784838603325177	20.869832632853385	24.281174589893695	28.06415417392774
37	26.634731799757855	20.431259229296593	24.493839747550165	28.440169223395383
38	25.903591425792992	19.964758978564483	25.72516990217292	28.406479693469606
39	25.384728336607164	19.254785381625354	26.78095987455083	28.579526407216655
40	25.1079533333518	19.930847806994457	27.58690430344958	27.374294556204166
41	23.66944365176773	19.401179687651513	28.431469788858628	28.49790687172213
42	22.35039881643362	20.14966352027883	29.25985543423754	28.240082229050017
43	21.77789844933965	20.297110624115163	29.12770160385435	28.79728932269084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	18.0
2	20.0
3	30.0
4	40.0
5	40.0
6	53.5
7	67.0
8	73.0
9	79.0
10	122.5
11	166.0
12	166.0
13	326.5
14	487.0
15	888.5
16	1290.0
17	1298.5
18	1307.0
19	1307.0
20	1589.0
21	1871.0
22	1758.0
23	1645.0
24	2048.0
25	2451.0
26	2451.0
27	3286.5
28	4122.0
29	5487.0
30	6852.0
31	8948.5
32	11045.0
33	11045.0
34	14787.0
35	18529.0
36	22116.0
37	25703.0
38	32884.5
39	40066.0
40	40066.0
41	47211.5
42	54357.0
43	64206.5
44	74056.0
45	92756.0
46	111456.0
47	111456.0
48	126221.5
49	140987.0
50	155744.0
51	170501.0
52	176815.0
53	183129.0
54	183129.0
55	178111.0
56	173093.0
57	169786.0
58	166479.0
59	159774.5
60	153070.0
61	153070.0
62	142851.0
63	132632.0
64	115005.0
65	97378.0
66	86243.5
67	75109.0
68	75109.0
69	65062.5
70	55016.0
71	46803.5
72	38591.0
73	31584.0
74	24577.0
75	24577.0
76	20180.0
77	15783.0
78	12763.0
79	9743.0
80	8020.5
81	6298.0
82	6298.0
83	4935.0
84	3572.0
85	2650.0
86	1728.0
87	1264.5
88	801.0
89	801.0
90	597.5
91	394.0
92	270.5
93	147.0
94	95.5
95	44.0
96	44.0
97	29.5
98	15.0
99	9.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1804715.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.15177518590797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.34635999215295	46.51851484036882
2	8.637075756044	9.527001207219895
3	2.6062618676613747	4.312199058025943
4	1.2655270903497864	2.7918426231459064
5	0.6999730428551767	1.9302377947872318
6	0.4605357250139326	1.5239617662628526
7	0.32173530413065365	1.2420991213948476
8	0.2371510612265073	1.0463441611091062
9	0.1768003259728392	0.8775766647764347
>10	1.0320770849290875	10.794157550512711
>50	0.1169085723740267	4.547340606248862
>100	0.08912174335876914	9.741713270392443
>500	0.007552236007884365	2.8567109712006125
>1k	0.0029201979230486213	2.2903003645543896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2782	0.15415176357485808	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2229	0.12350980625749772	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2119	0.11741466104066293	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1956	0.10838276403753501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.5410411062134465E-5	0.0	0.0	0.0
6	0.0	5.5410411062134465E-5	0.0	0.0	0.0
7	5.5410411062134465E-5	5.5410411062134465E-5	0.0	0.0	0.0
8	1.1082082212426893E-4	5.5410411062134465E-5	0.0	0.0	0.0
9	1.1082082212426893E-4	5.5410411062134465E-5	0.0	0.0	0.0
10	1.1082082212426893E-4	5.5410411062134465E-5	0.0	0.0	0.0
11	1.1082082212426893E-4	1.1082082212426893E-4	0.0	0.0	0.0
12	1.1082082212426893E-4	1.1082082212426893E-4	0.0	5.5410411062134465E-5	0.0
13	1.1082082212426893E-4	1.1082082212426893E-4	0.0	1.1082082212426893E-4	0.0
14	1.1082082212426893E-4	1.1082082212426893E-4	0.0	2.7705205531067235E-4	0.0
15	1.1082082212426893E-4	1.1082082212426893E-4	0.0	4.432832884970757E-4	0.0
16	1.1082082212426893E-4	1.1082082212426893E-4	0.0	9.419769880562858E-4	0.0
17	1.1082082212426893E-4	1.1082082212426893E-4	0.0	0.0016069019208018996	0.0
18	1.662312331864034E-4	1.662312331864034E-4	0.0	0.0018285435650504374	1.1082082212426893E-4
19	2.2164164424853786E-4	1.662312331864034E-4	0.0	0.002271826853547513	1.1082082212426893E-4
20	2.7705205531067235E-4	1.662312331864034E-4	0.0	0.00310298301947953	1.1082082212426893E-4
21	2.7705205531067235E-4	1.662312331864034E-4	0.0	0.004876116173467833	1.1082082212426893E-4
22	3.324624663728068E-4	1.662312331864034E-4	0.0	0.007591226315512421	1.1082082212426893E-4
23	3.324624663728068E-4	1.662312331864034E-4	0.0	0.00881025535887938	1.1082082212426893E-4
24	3.324624663728068E-4	1.662312331864034E-4	0.0	0.010971261390302625	1.1082082212426893E-4
25	3.324624663728068E-4	1.662312331864034E-4	0.0	0.012910625777477331	1.1082082212426893E-4
26	3.324624663728068E-4	1.662312331864034E-4	0.0	0.017288048251385954	1.1082082212426893E-4
27	3.324624663728068E-4	1.662312331864034E-4	0.0	0.04621228282582014	1.1082082212426893E-4
28	3.324624663728068E-4	1.662312331864034E-4	0.0	0.16861388086207518	1.1082082212426893E-4
29	3.324624663728068E-4	1.662312331864034E-4	0.0	0.34337831735204727	1.1082082212426893E-4
30	3.324624663728068E-4	1.662312331864034E-4	0.0	0.568677048730686	1.1082082212426893E-4
31	3.324624663728068E-4	1.662312331864034E-4	0.0	1.1968094685310422	1.1082082212426893E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	27.491621	1
TAGTACG	45	0.0038266412	20.555555	4
ACTATAT	85	1.2461132E-6	19.588236	3
CGTTATT	105	2.2615495E-8	19.38095	10
ATTCGCG	60	9.239597E-4	18.5	30
CTTATAC	2210	0.0	18.332579	37
GTATCAA	2655	0.0	18.3258	2
TATACAC	675	0.0	17.814814	37
TAATACG	1280	0.0	17.777344	27
ATACGGC	275	0.0	17.490908	29
GCGGTAA	1410	0.0	17.319149	23
GGTAATA	1400	0.0	17.310715	25
GGACGTA	215	0.0	17.209303	27
TAGCGAA	205	0.0	17.146341	10
ATCTAAG	490	0.0	16.989798	32
CGGTAAT	1435	0.0	16.759583	24
AACTCCG	1470	0.0	16.738094	5
GTAACGT	785	0.0	16.496815	26
GTCTAGT	90	4.4482178E-5	16.444445	1
AACGTCA	780	0.0	16.365385	28
>>END_MODULE