FastQCFastQC Report
Fri 10 Feb 2017
ERR1632575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22448
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA880.3920171062009979No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT720.3207412687099074TruSeq Adapter, Index 5 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA630.28064861012116893No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA530.23610121168923734No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA350.15591589451176052No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT350.15591589451176052No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC320.14255167498218105No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC300.13364219529579474No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC290.12918745545260157Illumina Paired End PCR Primer 2 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT290.12918745545260157No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC280.12473271560940842No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT280.12473271560940842No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC270.12027797576621525No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA260.1158232359230221No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA260.1158232359230221No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC260.1158232359230221No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG240.10691375623663579No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT230.10245901639344263No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA230.10245901639344263No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC230.10245901639344263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG200.001803681637.030
ATACGGA200.001803681637.029
AATACGG251.1954141E-437.028
ACTCCGT352.28621E-531.7142856
CCGTGCC303.4890184E-430.8333349
GCGGTAA303.4890184E-430.83333423
AACTCCG303.4890184E-430.8333345
TAATACG303.4890184E-430.83333427
GTAATAC303.4890184E-430.83333426
CGGTAAT303.4890184E-430.83333424
GGTAATA303.4890184E-430.83333425
GTGCCAG250.00538348429.611
TCTTATA405.688189E-527.75000237
CTCCGTG405.688189E-527.7500027
TAACTCC358.599747E-426.428574
CTAACTC358.599747E-426.428573
GCTAACT358.599747E-426.428572
CGCGGTA358.599747E-426.4285722
CGTGCCA358.599747E-426.4285710
GGCTAAC358.599747E-426.428571
Produced by FastQC (version 0.11.5)