FastQCFastQC Report
Fri 10 Feb 2017
ERR1632574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636745
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA27760.4359673024523161No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25600.40204477459579574No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA22230.34911934919002113No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18510.29069721788156955No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18300.2873991943399634No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17590.27624873379453313No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17440.2738930026933859No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16590.2605438597868848No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA15630.24546718073954252No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC15160.23808588995594782No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14640.22991935547197073No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14630.22976230673189424No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14520.22803477059105295No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC14350.22536494200975274No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14340.22520789326967625No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA13790.2165702125654697No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13680.2148426764246284No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13300.20887482430172205No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13290.20871777556164556No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13150.2065190932005748No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13030.20463450831965702No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12890.20243582595858622No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12410.19489748643491508No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12110.1901860242326206No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA12070.18955782927231468No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11920.18720209817116742No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11860.18625980573070852No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11580.18186244100856702No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA11190.17573754014558418No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC11040.17338180904443695No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA10890.1710260779432897No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10730.16851329810206597No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC10370.1628595434593126No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC10280.16144610479862426No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10210.16034676361808886No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10070.15814808125701812No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC10050.15783398377686517No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9860.154850057715412No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT9610.15092383921349992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9450.14841105937227617No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9440.1482540106321997No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9390.1474687669318173No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA9340.14668352323143488No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC9300.14605532827112894No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG9230.14495598709059357No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG9180.14417074339021116No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9150.1436995971699817No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC9020.14165796354898744No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9000.14134386606883448No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8990.14118681732875799No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8830.13867403748753426No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC8800.1382028912673048No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8730.13710355008676944No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8690.1364753551264635No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC8620.13537601394592813No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8580.1347478189856222No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC8540.13411962402531627No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8470.13302028284478087No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8450.13270618536462792No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8450.13270618536462792No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8360.13129274670393956No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8330.13082160048371014No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT8320.13066455174363364No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8300.13035045426348066No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7970.12516784584095675No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7950.12485374836080378No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA7930.12453965088065082No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7910.12422555340049785No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7750.12171277355927411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.12108457859896819No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7580.11904294497797391No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC7560.11872884749782095No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT7540.11841475001766798No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7520.11810065253751502No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT7480.11747245757720909No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC7450.11700131135697965No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7400.11621606765659723No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7350.1154308239562148No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC7180.1127609953749146No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7110.11166165419437923No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7040.11056231301384385No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7000.10993411805353792No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6970.10946297183330846No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6920.10867772813292606No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6900.1083636306527731No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC6890.10820658191269661No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6860.10773543569246717No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG6850.10757838695239068No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT6820.10710724073216121No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC6810.10695019199208475No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC6800.10679314325200825No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA6770.10632199703177882No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA6710.10537970459131993No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG6640.10428036341078453No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6440.1011393886092549No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6430.1009823398691784No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA6390.10035414490887247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGA200.001841319537.01
CTTTGTT401.5985843E-632.3759
ATTGGCT401.5985843E-632.37534
GGTATCA13550.032.3579331
ATACGGC1550.031.03225929
GTCATAG250.005494525729.5999981
CCTTATC502.717552E-729.5999982
TTGGACC250.005494525729.59999835
AGTGTCT250.005494525729.59999834
TATCCTT454.004565E-628.7777795
CTTTTTA652.9849616E-928.4615382
TTTTACG652.9849616E-928.4615384
TACGGCT1750.027.48571430
CTTATCC556.254377E-726.909093
CCCTTAT556.254377E-726.909091
TTTACGA706.5811037E-926.4285725
TTTTTAC706.5811037E-926.4285723
TGACTCT358.8650314E-426.42857233
AATATGC509.082776E-625.89999837
TAAGTCT1051.8189894E-1224.66666635
Produced by FastQC (version 0.11.5)