FastQCFastQC Report
Fri 10 Feb 2017
ERR1632573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061085
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA29440.27745185352728574No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA23730.22363901101231284No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17940.16907222324318974No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17410.16407733593444446No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17380.16379460646413813No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16920.15945942125277426No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA16290.1535221023763412No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15850.1493754034785149No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15770.14862145822436468No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14260.13439074155227904No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13600.12817069320553962No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT13390.12619158691339524No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT13250.12487218271863235No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12980.12232761748587531No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12550.1182751617448178No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12400.11686151439328611No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC12270.11563635335529199No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12210.11507089441467931No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11910.11224359971161593No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11700.11026449341947157No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11200.10555233558103261No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC11170.10526960611072628No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10890.10263079772120046No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10740.10121715036966877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.029.6474361
CCTATAT250.005496155529.61
TCTAGAC451.3231185E-424.6666663
CTAAAGT551.9024801E-523.5454544
ATACGGC1350.023.29629729
ATAGGAC400.001931089823.1253
TATCTAT400.001931089823.1255
GCCTATA502.7019126E-422.235
GTATCAA21950.020.902052
CTCGTAT909.476571E-820.55555529
TCGTATG909.476571E-820.55555530
TATAAGA555.142736E-420.18181812
ATACACA2300.020.10869637
AACGACC1202.382876E-1020.04166837
TCTAAAG656.901491E-519.9230772
TTGAATA759.26467E-619.7333344
TACGGCT1800.019.52777730
GGTAATA11900.019.1218525
TTCTGCG6400.019.07812518
GCGGTAA12100.018.65289323
Produced by FastQC (version 0.11.5)